Rabbit Anti-HSP90AB1 Recombinant Antibody (
7G7) (V2LY-0725-LY1452)

Name: Rabbit Anti-HSP90AB1 Recombinant Antibody (7G7)
SKU: V2LY-0725-LY1452
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-HSP90AB1 Recombinant Antibody (7G7) (V2LY-0725-LY1452) in WB

Western Blot
Positive WB detected in: HepG2 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate
All lanes: HSP90AB1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 84 kDa
Observed band size: 80-90 kDa

Rabbit Anti-HSP90AB1 Recombinant Antibody (7G7) (V2LY-0725-LY1452) in IHC

IHC image of V2LY-0725-LY1452 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-HSP90AB1 Recombinant Antibody (7G7) (V2LY-0725-LY1452) in IHC

IHC image of V2LY-0725-LY1452 diluted at 1:100 and staining in paraffin-embedded human colon tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-HSP90AB1 Recombinant Antibody (7G7) (V2LY-0725-LY1452) in IF

Immunofluorescence staining of HepG2 cell with V2LY-0725-LY1452 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 495-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-HSP90AB1 Recombinant Antibody (7G7) (V2LY-0725-LY1452) in FC

Overlay Peak curve showing Hela cells stained with V2LY-0725-LY1452 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

7G7

Application

ELISA, WB, IHC, IF, FC

Immunogen

A synthesized peptide derived from human HSP90AB1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

heat shock protein 90 alpha family class B member 1

Entrez Gene ID

3326

UniProt ID

P08238

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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