Mouse Anti-HSPA8 Recombinant Antibody (A304) (CBMAB-AP9842LY)

Name: Mouse Anti-HSPA8 Recombinant Antibody (A304)
SKU: CBMAB-AP9842LY
Rating: 5 (1 reviews)

See all HSPA8 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

A304

Application

ICC/IF, PLA, PBA, AM, ELISA, IHC, IP, WB

Immunogen

Full length human HSC70

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

Affinity purity

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

heat shock protein family A (Hsp70) member 8

Introduction

This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Entrez Gene ID

Human	3312
Mouse	15481
Rat	24468

UniProt ID

Human	P11142
Mouse	P63017
Rat	P63018

Alternative Names

Heat Shock Protein Family A (Hsp70) Member 8; Lipopolysaccharide-Associated Protein 1; Heat Shock 70kDa Protein 8; LPS-Associated Protein 1; HSPA10; HSC70; HSP73; LAP-1; Epididymis Secretory Sperm Binding Protein Li 72p; N-Myristoyltransferase Inhibitor Protein 71; Constitutive Heat Shock Protein 70; Heat Shock Cognate 71 KDa Protein; Epididymis Luminal Protein 33;

Function

SMC-151A

Biological Process

ATP metabolic process Source: BHF-UCL
Cellular response to starvation Source: ParkinsonsUK-UCL
Cellular response to steroid hormone stimulus Source: Reactome
Cellular response to unfolded protein Source: GO_Central
Chaperone cofactor-dependent protein refolding Source: GO_Central
Chaperone-mediated autophagy Source: ParkinsonsUK-UCL
Chaperone-mediated autophagy translocation complex disassembly Source: ParkinsonsUK-UCL
Late endosomal microautophagy Source: GO_Central
Membrane organization Source: Reactome
mRNA processing Source: UniProtKB-KW
Negative regulation of supramolecular fiber organization Source: BHF-UCL
Negative regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation by host of viral genome replication Source: Ensembl
Positive regulation of mRNA splicing, via spliceosome Source: Ensembl
Protein folding Source: UniProtKB
Protein refolding Source: UniProtKB
Protein targeting to lysosome involved in chaperone-mediated autophagy Source: ParkinsonsUK-UCL
Regulation of cell cycle Source: Ensembl
Regulation of postsynapse organization Source: Ensembl
Regulation of protein complex stability Source: ParkinsonsUK-UCL
Regulation of protein-containing complex assembly Source: ParkinsonsUK-UCL
Regulation of protein import Source: ParkinsonsUK-UCL
Regulation of protein stability Source: ParkinsonsUK-UCL
Response to unfolded protein Source: UniProtKB
RNA splicing Source: UniProtKB-KW
Slow axonal transport Source: GO_Central
Vesicle-mediated transport Source: GO_Central

Cellular Location

Nucleolus; Cytoplasm; Cell membrane; Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock.

PTM

Acetylated.
ISGylated.
Trimethylation at Lys-561 reduces fibrillar SNCA binding.

Wang, Y., Zhao, M., Zhao, L., Geng, Y., Li, G., Chen, L., ... & Zhang, X. (2023). HBx-induced HSPA8 stimulates HBV replication and suppresses ferroptosis to support liver cancer progression. Cancer Research, 83(7), 1048-1061.

Wang, L., Li, R., Geng, R., Zhang, L., Chen, X. X., Qiao, S., & Zhang, G. (2022). Heat shock protein member 8 (HSPA8) is involved in porcine reproductive and respiratory syndrome virus attachment and internalization. Microbiology Spectrum, 10(1), e01860-21.

Wang, C., Zhang, X., Wang, X., Zhai, Y., Li, M., Pan, J., ... & Zhou, J. (2022). Genetic deletion of hspa8 leads to selective tissue malformations in zebrafish embryonic development. Journal of Cell Science, 135(21), jcs259734.

Zhang, Y., Qiao, X., Liu, L., Han, W., Liu, Q., Wang, Y., ... & Chen, C. (2022). Long noncoding RNA MAGI2-AS3 regulates the H2O2 level and cell senescence via HSPA8. Redox Biology, 54, 102383.

Li, J., & Ge, Z. (2021). High HSPA8 expression predicts adverse outcomes of acute myeloid leukemia. BMC cancer, 21(1), 1-11.

Wang, C., Arrington, J., Ratliff, A. C., Chen, J., Horton, H. E., Nie, Y., ... & Kuang, S. (2019). Methyltransferase-like 21c methylates and stabilizes the heat shock protein Hspa8 in type I myofibers in mice. Journal of Biological Chemistry, 294(37), 13718-13728.

Bonam, S. R., Ruff, M., & Muller, S. (2019). HSPA8/HSC70 in immune disorders: a molecular rheostat that adjusts chaperone-mediated autophagy substrates. Cells, 8(8), 849.

Khosla, R., Hemati, H., Rastogi, A., Ramakrishna, G., Sarin, S. K., & Trehanpati, N. (2019). miR‐26b‐5p helps in EpCAM+ cancer stem cells maintenance via HSC71/HSPA8 and augments malignant features in HCC. Liver International, 39(9), 1692-1703.

Tian, Y., Xu, H., Farooq, A. A., Nie, B., Chen, X., Su, S., ... & Lin, X. (2018). Maslinic acid induces autophagy by down‐regulating HSPA8 in pancreatic cancer cells. Phytotherapy Research, 32(7), 1320-1331.

Xiang, X., You, X. M., & Li, L. Q. (2018). Expression of HSP90AA1/HSPA8 in hepatocellular carcinoma patients with depression. OncoTargets and therapy, 3013-3023.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-HSPA8 Recombinant Antibody (A304)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-HSPA8 (AA 534-615) Recombinant Antibody (CBFYH-2296) (CAT#: CBMAB-H3310-FY)

Mouse Anti-HSPA8 Recombinant Antibody (A312) (CAT#: CBMAB-AP10554LY)

Mouse Anti-HSPA8 Recombinant Antibody (A305) (CAT#: CBMAB-AP9845LY)

Rat Anti-HSPA8 Recombinant Antibody (CBFYH-2308) (CAT#: CBMAB-H3321-FY)

Mouse Anti-HSPA8 Recombinant Antibody (CBFYH-2304) (CAT#: CBMAB-H3317-FY)

Mouse Anti-HSPA8 Recombinant Antibody (CBFYH-2298) (CAT#: CBMAB-H3311-FY)

Mouse Anti-HSPA8 Recombinant Antibody (A302) (CAT#: CBMAB-AP9864LY)

Mouse Anti-HSPA8 Recombinant Antibody (CBT738) (CAT#: V2LY-0625-LY3316)

Mouse Anti-HSPA8 Recombinant Antibody (A310) (CAT#: CBMAB-AP3222LY)

Recombinant Rabbit Anti-HSPA8Antibody (CBAb124) (CAT#: CBMAB-Ab124-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry