Mouse Anti-HYAL4 (AA 357-456) Recombinant Antibody (CBFYH-2565) (CBMAB-H3587-FY)

This product is mouse antibody that recognizes HYAL4. The antibody CBFYH-2565 can be used for immunoassay techniques such as: ELISA, WB.
See all HYAL4 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYH-2565

Antibody Isotype

IgG2a, κ

Application

ELISA, WB

Basic Information

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen sequence: FVSSDLGSYI ANVTRAAEVC SLHLCRNNGR CIRKMWNAPS YLHLNPASYH IEASEDGEFT VKGKASDTDL AVMADTFSCH CYQGYEGADC REIKTADGCS

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 357-456

Target

Full Name

Hyaluronoglucosaminidase 4

Introduction

This gene encodes a protein which is similar in structure to hyaluronidases. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. However, this protein has not yet been shown to have hyaluronidase activity.

Entrez Gene ID

23553

UniProt ID

Q2M3T9

Alternative Names

Hyaluronoglucosaminidase 4; Chondroitin Sulfate Endo-Beta-N-Acetylgalactosaminidase; Chondroitin Sulfate Hydrolase; EC 3.2.1.35; Hyal-4; CSHY

Function

Endo-hyaluronidase that degrades hyaluronan to smaller oligosaccharide fragments. Has also chondroitin sulfate hydrolase activity, The best substrate being the galactosaminidic linkage in the sequence of a trisulfated tetrasaccharide.

Biological Process

Carbohydrate metabolic process Source: InterPro
Chondroitin sulfate catabolic process Source: Ensembl
Glycosaminoglycan catabolic process Source: ProtInc
Hyaluronan catabolic process Source: GO_Central

Cellular Location

Membrane

Topology

Cytoplasmic: 1-8
Helical: 9-29
Extracellular: 30-453
Helical: 454-474
Cytoplasmic: 475-481

References

Hasanali, S. L., Morera, D. S., Racine, R. R., Hennig, M., Ghosh, S., Lopez, L. E., ... & Lokeshwar, V. B. (2021). HYAL4-V1/Chondroitinase (Chase) drives gemcitabine resistance and predicts chemotherapy failure in patients with bladder cancer. Clinical Cancer Research, 27(15), 4410-4421.

Lokeshwar, V. B., Morera, D. S., Hasanali, S. L., Yates, T. J., Hupe, M. C., Knapp, J., ... & Lokeshwar, B. L. (2020). A novel splice variant of HYAL-4 drives malignant transformation and predicts outcome in patients with bladder cancer. Clinical Cancer Research, 26(13), 3455-3467.

Quirós, L. M., Merayo-Lloves, J., Alcalde, I., Martín, C., Vázquez, F., Alfonso, J., ... & García, B. (2019). HYAL4 and PH20, involved in the hydrolysis of chondroitin sulfate chains, undergo a subexpression in the cornea of keratoconus patients. Investigative Ophthalmology & Visual Science, 60(9), 5107-5107.

Morera, D. S., Lahorewala, S. S., Lokeshwar, S. D., Jordan, A. R., & Lokeshwar, V. B. (2019). HYAL4: The first study on a potential molecular driver and biomarker of invasive bladder cancer. Cancer Research, 79(13_Supplement), 1259-1259.

Lahorewala, S. S., Morera, D. S., Jordan, A. R., & Lokeshwar, V. B. (2018). HYAL4: A molecular driver and potential marker of invasive bladder cancer. Cancer Research, 78(13_Supplement), 2655-2655.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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