Mouse Anti-IDH1 Recombinant Antibody (CBT3096) (V2LY-0625-LY598)

Name: Mouse Anti-IDH1 Recombinant Antibody (CBT3096)
SKU: V2LY-0625-LY598
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-IDH1 Recombinant Antibody (CBT3096) (V2LY-0625-LY598) in ELISA

Mouse Anti-IDH1 Recombinant Antibody (CBT3096) (V2LY-0625-LY598) in WB

Mouse Anti-IDH1 Recombinant Antibody (CBT3096) (V2LY-0625-LY598) in IF

Mouse Anti-IDH1 Recombinant Antibody (CBT3096) (V2LY-0625-LY598) in FC

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT3096

Application

WB, ICC, FC

Immunogen

Purified recombinant fragment of human IDH1 (AA: 156-298) expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Mouse, Monkey

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:50-1:250
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Isocitrate Dehydrogenase (NADP(+)) 1, Cytosolic

Entrez Gene ID

3417

UniProt ID

O75874

Biological Process

2-oxoglutarate metabolic process Source: UniProtKB
Female gonad development Source: Ensembl
Glutathione metabolic process Source: Ensembl
Glyoxylate cycle Source: UniProtKB-KW
Isocitrate metabolic process Source: UniProtKB
NADP metabolic process Source: GO_Central
Regulation of phospholipid biosynthetic process Source: Ensembl
Regulation of phospholipid catabolic process Source: Ensembl
Response to oxidative stress Source: Ensembl
Response to steroid hormone Source: Ensembl
Tricarboxylic acid cycle Source: UniProtKB-KW

Cellular Location

Peroxisome; Cytosol

Involvement in disease

Glioma (GLM):
The gene represented in this entry is involved in disease pathogenesis. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. Elevated levels of R(-)-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. Gliomas are benign or malignant central nervous system neoplasms derived from glial cells. They comprise astrocytomas and glioblastoma multiforme that are derived from astrocytes, oligodendrogliomas derived from oligodendrocytes and ependymomas derived from ependymocytes. Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.

PTM

Acetylation at Lys-374 dramatically reduces catalytic activity.

Sabo, K. A., Albekioni, E., Caliger, D., Coleman, N. J., Thornberg, E., Avellaneda Matteo, D., ... & Sohl, C. D. (2023). Capturing the Dynamic Conformational Changes of Human Isocitrate Dehydrogenase 1 (IDH1) upon Ligand and Metal Binding Using Hydrogen–Deuterium Exchange Mass Spectrometry. Biochemistry, 62(6), 1145-1159.

Reinbold, R., Hvinden, I. C., Rabe, P., Herold, R. A., Finch, A., Wood, J., ... & Schofield, C. J. (2022). Resistance to the isocitrate dehydrogenase 1 mutant inhibitor ivosidenib can be overcome by alternative dimer-interface binding inhibitors. Nature Communications, 13(1), 4785.

Herold, R. A., Reinbold, R., Megarity, C. F., Abboud, M. I., Schofield, C. J., & Armstrong, F. A. (2021). Exploiting electrode nanoconfinement to investigate the catalytic properties of isocitrate dehydrogenase (IDH1) and a cancer-associated variant. The Journal of Physical Chemistry Letters, 12(26), 6095-6101.

Liu, S., Abboud, M. I., John, T., Mikhailov, V., Hvinden, I., Walsby-Tickle, J., ... & Schofield, C. J. (2021). Roles of metal ions in the selective inhibition of oncogenic variants of isocitrate dehydrogenase 1. Communications Biology, 4(1), 1243.

Bruce-Brand, C., & Govender, D. (2020). Gene of the month: IDH1. Journal of Clinical Pathology, 73(10), 611-615.

Bhavya, B., Anand, C. R., Madhusoodanan, U. K., Rajalakshmi, P., Krishnakumar, K., Easwer, H. V., ... & Gopala, S. (2020). To be wild or mutant: role of isocitrate dehydrogenase 1 (IDH1) and 2-hydroxy glutarate (2-HG) in gliomagenesis and treatment outcome in glioma. Cellular and molecular neurobiology, 40, 53-63.

Luna, L. A., Lesecq, Z., White, K. A., Hoang, A., Scott, D. A., Zagnitko, O., ... & Sohl, C. D. (2020). An acidic residue buried in the dimer interface of isocitrate dehydrogenase 1 (IDH1) helps regulate catalysis and pH sensitivity. Biochemical journal, 477(16), 2999-3018.

Verdura, S., Cuyàs, E., Lozano-Sánchez, J., Bastidas-Velez, C., Llorach-Parés, L., Fernández-Arroyo, S., ... & Menendez, J. A. (2019). An olive oil phenolic is a new chemotype of mutant isocitrate dehydrogenase 1 (IDH1) inhibitors. Carcinogenesis, 40(1), 27-40.

Jakob, C. G., Upadhyay, A. K., Donner, P. L., Nicholl, E., Addo, S. N., Qiu, W., ... & Hutti, J. E. (2018). Novel modes of inhibition of wild-type isocitrate dehydrogenase 1 (IDH1): direct covalent modification of His315. Journal of Medicinal Chemistry, 61(15), 6647-6657.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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