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Mouse Anti-IDH1 Recombinant Antibody (CBT3096) (V2LY-0625-LY598)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Monkey
Clone
CBT3096
Antibody Isotype
IgG1
Application
WB, ICC, FC

Basic Information

Immunogen
Purified recombinant fragment of human IDH1 (AA: 156-298) expressed in E. Coli.
Host Species
Mouse
Specificity
Human, Mouse, Monkey
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
ICC1:50-1:250
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Isocitrate Dehydrogenase (NADP(+)) 1, Cytosolic
Entrez Gene ID
3417
UniProt ID
O75874
Biological Process
2-oxoglutarate metabolic process Source: UniProtKB
Female gonad development Source: Ensembl
Glutathione metabolic process Source: Ensembl
Glyoxylate cycle Source: UniProtKB-KW
Isocitrate metabolic process Source: UniProtKB
NADP metabolic process Source: GO_Central
Regulation of phospholipid biosynthetic process Source: Ensembl
Regulation of phospholipid catabolic process Source: Ensembl
Response to oxidative stress Source: Ensembl
Response to steroid hormone Source: Ensembl
Tricarboxylic acid cycle Source: UniProtKB-KW
Cellular Location
Peroxisome; Cytosol
Involvement in disease
Glioma (GLM):
The gene represented in this entry is involved in disease pathogenesis. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. Elevated levels of R(-)-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. Gliomas are benign or malignant central nervous system neoplasms derived from glial cells. They comprise astrocytomas and glioblastoma multiforme that are derived from astrocytes, oligodendrogliomas derived from oligodendrocytes and ependymomas derived from ependymocytes. Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
PTM
Acetylation at Lys-374 dramatically reduces catalytic activity.
More Infomation

Sabo, K. A., Albekioni, E., Caliger, D., Coleman, N. J., Thornberg, E., Avellaneda Matteo, D., ... & Sohl, C. D. (2023). Capturing the Dynamic Conformational Changes of Human Isocitrate Dehydrogenase 1 (IDH1) upon Ligand and Metal Binding Using Hydrogen–Deuterium Exchange Mass Spectrometry. Biochemistry, 62(6), 1145-1159.

Reinbold, R., Hvinden, I. C., Rabe, P., Herold, R. A., Finch, A., Wood, J., ... & Schofield, C. J. (2022). Resistance to the isocitrate dehydrogenase 1 mutant inhibitor ivosidenib can be overcome by alternative dimer-interface binding inhibitors. Nature Communications, 13(1), 4785.

Herold, R. A., Reinbold, R., Megarity, C. F., Abboud, M. I., Schofield, C. J., & Armstrong, F. A. (2021). Exploiting electrode nanoconfinement to investigate the catalytic properties of isocitrate dehydrogenase (IDH1) and a cancer-associated variant. The Journal of Physical Chemistry Letters, 12(26), 6095-6101.

Liu, S., Abboud, M. I., John, T., Mikhailov, V., Hvinden, I., Walsby-Tickle, J., ... & Schofield, C. J. (2021). Roles of metal ions in the selective inhibition of oncogenic variants of isocitrate dehydrogenase 1. Communications Biology, 4(1), 1243.

Bruce-Brand, C., & Govender, D. (2020). Gene of the month: IDH1. Journal of Clinical Pathology, 73(10), 611-615.

Bhavya, B., Anand, C. R., Madhusoodanan, U. K., Rajalakshmi, P., Krishnakumar, K., Easwer, H. V., ... & Gopala, S. (2020). To be wild or mutant: role of isocitrate dehydrogenase 1 (IDH1) and 2-hydroxy glutarate (2-HG) in gliomagenesis and treatment outcome in glioma. Cellular and molecular neurobiology, 40, 53-63.

Luna, L. A., Lesecq, Z., White, K. A., Hoang, A., Scott, D. A., Zagnitko, O., ... & Sohl, C. D. (2020). An acidic residue buried in the dimer interface of isocitrate dehydrogenase 1 (IDH1) helps regulate catalysis and pH sensitivity. Biochemical journal, 477(16), 2999-3018.

Verdura, S., Cuyàs, E., Lozano-Sánchez, J., Bastidas-Velez, C., Llorach-Parés, L., Fernández-Arroyo, S., ... & Menendez, J. A. (2019). An olive oil phenolic is a new chemotype of mutant isocitrate dehydrogenase 1 (IDH1) inhibitors. Carcinogenesis, 40(1), 27-40.

Jakob, C. G., Upadhyay, A. K., Donner, P. L., Nicholl, E., Addo, S. N., Qiu, W., ... & Hutti, J. E. (2018). Novel modes of inhibition of wild-type isocitrate dehydrogenase 1 (IDH1): direct covalent modification of His315. Journal of Medicinal Chemistry, 61(15), 6647-6657.

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For research use only. Not intended for any clinical use.

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