Mouse Anti-IFNK Recombinant Antibody (1B7) (CBMAB-A4287-LY)

The product is antibody recognizes IFNK. The antibody 1B7 immunoassay techniques such as: WB, ELISA.
See all IFNK antibodies

Published Data

Fig.1 Immunofluorescent colocalisation with the epidermal marker cytokeratin 14 showed colocalisation with IFN-κ.

Fig.2 Western Blot of IFN-κ overexpressing line (IFN-κ OE) (both native and GFP tagged) is shown.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1B7

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

IFNK (NP_064509, 35 a.a. ~ 129 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Interferon Kappa

Introduction

This gene encodes a member of the type I interferon family. Type I interferons are a group of related glycoproteins that play an important role in host defenses against viral infections. This protein is expressed in keratinocytes and the gene is found on chromosome 9, adjacent to the type I interferon cluster. [provided by RefSeq]

Entrez Gene ID

56832

UniProt ID

Q9P0W0

Alternative Names

RP11-27J8.1

Function

May play a role in the regulation of immune cell function. Cytokine that imparts cellular protection against viral infection in a species-specific manner. Activates the interferon-stimulated response element signaling pathway. It is able to directly modulate cytokine release from monocytes and dendritic cells. Binds heparin.

Biological Process

Adaptive immune response Source: GO_Central
B cell differentiation Source: GO_Central
B cell proliferation Source: GO_Central
Cytokine-mediated signaling pathway Source: UniProtKB
Defense response to virus Source: UniProtKB-KW
Humoral immune response Source: GO_Central
Natural killer cell activation Source: UniProtKB
Natural killer cell activation involved in immune response Source: GO_Central
Negative regulation of cell population proliferation Source: UniProtKB
Positive regulation of innate immune response Source: UniProtKB
Positive regulation of peptidyl-serine phosphorylation of STAT protein Source: GO_Central
Regulation of transcription, DNA-templated Source: UniProtKB
Response to exogenous dsRNA Source: GO_Central
Response to virus Source: UniProtKB
T cell activation involved in immune response Source: GO_Central

Cellular Location

Secreted

References

Wolf, S., Audu, C. O., Joshi, A. D., denDekker, A., Melvin, W. J., Xing, X., ... & Gallagher, K. A. (2022). Interferon kappa (IFNk) in keratinocytes is critical for normal wound repair and is decreased in diabetic wounds. The Journal of Immunology, 208(1_Supplement), 45-09.

Klein, K. (2022). Regulation of Interferon-kappa Expression in Normal and HPV-positive Human Keratinocytes (Doctoral dissertation, Universität Tübingen).

Li, Y., Song, Y., Zhu, L., Wang, X., Richers, B., Leung, D. Y., & Bin, L. (2020). Interferon kappa is important for keratinocyte host defense against herpes simplex virus-1. Journal of Immunology Research, 2020.

Li, Y., Song, Y., Zhu, L., Wang, X., Yang, B., Lu, P., ... & Deng, L. (2019). Interferon kappa is up-regulated in psoriasis and it up-regulates psoriasis-associated cytokines in vivo. Clinical, cosmetic and investigational dermatology, 865-873.

Gao, M., Guo, Y., Du, J., Song, Z., Luo, X., Wang, J., & Han, W. (2018). Evolutional conservation of molecular structure and antiviral function of a type I interferon, IFN-kappa, in poultry. Developmental & Comparative Immunology, 89, 44-53.

Sarkar, M. K., Hile, G. A., Tsoi, L. C., Xing, X., Liu, J., Liang, Y., ... & Kahlenberg, J. M. (2018). Photosensitivity and type I IFN responses in cutaneous lupus are driven by epidermal-derived interferon kappa. Annals of the rheumatic diseases, 77(11), 1653-1664.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Antibody Pairs

IFNK Matched Antibody Pair (605)

Related Products

IFNK Matched Antibody Pair (605)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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