Mouse Anti-IFT20 Recombinant Antibody (3F3) (CBMAB-A4290-LY)

Name: Mouse Anti-IFT20 Recombinant Antibody (3F3)
SKU: CBMAB-A4290-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3F3

Application

WB, ELISA

Immunogen

IFT20 (NP_777547.1, 1 a.a. ~ 148 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Intraflagellar Transport 20

Entrez Gene ID

90410

UniProt ID

Q8IY31

Function

Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium (PubMed:16775004).

Regulates the platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway. Required for protein stability of E3 ubiquitin ligases CBL and CBLB that mediate ubiquitination and internalization of PDGFRA for proper feedback inhibition of PDGFRA signaling (PubMed:29237719).

Essential for male fertility. Plays an important role in spermatogenesis, particularly spermiogenesis, when germ cells form flagella. May play a role in the transport of flagellar proteins ODF2 and SPAG16 to build sperm flagella and in the removal of redundant sperm cytoplasm (By similarity).

Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment (By similarity).

Biological Process

Cardiac muscle cell differentiation Source: Ensembl
Centrosome localization Source: GO_Central
Cilium assembly Source: UniProtKB
Cochlea development Source: Ensembl
Establishment of epithelial cell apical/basal polarity Source: Ensembl
Establishment of planar polarity Source: Ensembl
Inner ear receptor cell stereocilium organization Source: Ensembl
Intraciliary transport Source: GO_Central
Kidney development Source: Ensembl
Neural precursor cell proliferation Source: Ensembl
Opsin transport Source: Ensembl
Photoreceptor cell outer segment organization Source: Ensembl
Protein localization to ciliary membrane Source: Ensembl
Protein localization to cilium Source: GO_Central
Protein localization to Golgi apparatus Source: MGI
Protein localization to plasma membrane Source: Ensembl
Regulation of autophagosome assembly Source: UniProtKB
Regulation of canonical Wnt signaling pathway Source: Ensembl
Regulation of cilium assembly Source: UniProtKB
Regulation of platelet-derived growth factor receptor-alpha signaling pathway Source: UniProtKB
Smoothened signaling pathway Source: Ensembl
Spermatogenesis Source: UniProtKB
Visual learning Source: Ensembl

Cellular Location

Cis-Golgi network; Golgi apparatus; Centriole; Cilium basal body; Cytoskeleton; Cilium; Acrosome. Present at the centrosomes during the cell cycle and associated with the proximal portion of the mother centriole and the lateral aspect of the daughter centriole. Associated with basal body at the base of primary cilia. Detected in the Golgi apparatus of round spermatids and late spermatocytes. Also detected in the manchette of step 10-12 spermatids. In step 14 spermatids, found in the basal body of the sperm tail.

Finetti, F., Onnis, A., & Baldari, C. T. (2022). IFT20: An Eclectic Regulator of Cellular Processes beyond Intraflagellar Transport. International Journal of Molecular Sciences, 23(20), 12147.

Li, Y., Yang, S., Liu, Y., Qin, L., & Yang, S. (2022). IFT20 governs mesenchymal stem cell fate through positively regulating TGF-β-Smad2/3-Glut1 signaling mediated glucose metabolism. Redox Biology, 54, 102373.

Boukhalfa, A., Roccio, F., Dupont, N., Codogno, P., & Morel, E. (2021). The autophagy protein ATG16L1 cooperates with IFT20 and INPP5E to regulate the turnover of phosphoinositides at the primary cilium. Cell Reports, 35(4).

Yang, H., Zhang, F., Long, H., Lin, Y., Liao, J., Xia, H., & Huang, K. (2021). IFT20 mediates the transport of cell migration regulators from the trans-golgi network to the plasma membrane in breast cancer cells. Frontiers in Cell and Developmental Biology, 9, 632198.

Huang, Q., Liu, H., Zeng, J., Li, W., Zhang, S., Zhang, L., ... & Zhang, Z. (2020). COP9 signalosome complex subunit 5, an IFT20 binding partner, is essential to maintain male germ cell survival and acrosome biogenesis. Biology of reproduction, 102(1), 233-247.

Yamaguchi, H., Terajima, M., Kitami, M., Wang, J., He, L., Saeki, M., ... & Komatsu, Y. (2020). IFT20 is critical for collagen biosynthesis in craniofacial bone formation. Biochemical and biophysical research communications, 533(4), 739-744.

Finetti, F., Cassioli, C., Cianfanelli, V., Onnis, A., Paccagnini, E., Kabanova, A., & Baldari, C. T. (2020). The intraflagellar transport protein IFT20 controls lysosome biogenesis by regulating the post-Golgi transport of acid hydrolases. Cell Death & Differentiation, 27(1), 310-328.

Kitami, M., Yamaguchi, H., Ebina, M., Kaku, M., Chen, D., & Komatsu, Y. (2019). IFT20 is required for the maintenance of cartilaginous matrix in condylar cartilage. Biochemical and biophysical research communications, 509(1), 222-226.

Martin, L., Kaci, N., Estibals, V., Goudin, N., Garfa-Traore, M., Benoist-Lasselin, C., ... & Legeai-Mallet, L. (2018). Constitutively-active FGFR3 disrupts primary cilium length and IFT20 trafficking in various chondrocyte models of achondroplasia. Human molecular genetics, 27(1), 1-13.

Schmid, F. M., Schou, K. B., Vilhelm, M. J., Holm, M. S., Breslin, L., Farinelli, P., ... & Christensen, S. T. (2018). IFT20 modulates ciliary PDGFRα signaling by regulating the stability of Cbl E3 ubiquitin ligases. Journal of Cell Biology, 217(1), 151-161.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
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Isotype control

For research use only. Not intended for any clinical use.

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