Rabbit Anti-ILF3 Recombinant Antibody (
7H8) (V2LY-0725-LY1172)

Name: Rabbit Anti-ILF3 Recombinant Antibody (7H8)
SKU: V2LY-0725-LY1172
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-ILF3 Recombinant Antibody (7H8) (V2LY-0725-LY1172) in IHC

IHC image of V2LY-0725-LY1172 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-ILF3 Recombinant Antibody (7H8) (V2LY-0725-LY1172) in IHC

IHC image of V2LY-0725-LY1172 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-ILF3 Recombinant Antibody (7H8) (V2LY-0725-LY1172) in IF

Immunofluorescence staining of Hela Cells with V2LY-0725-LY1172 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

7H8

Application

ELISA, IHC, IF

Immunogen

A synthesized peptide derived from human ILF3.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:50-1:200
IF	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

interleukin enhancer binding factor 3

Entrez Gene ID

3609

UniProt ID

Q12906

Function

RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs (PubMed:28625552).
As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:14731398).
Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response (PubMed:21123651, PubMed:34110282).
Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs (PubMed:21123651, PubMed:28625552).
(Microbial infection) Plays a positive role in HIV-1 virus production by binding to and thereby stabilizing HIV-1 RNA, together with ILF3.

Biological Process

Defense response to virusIEA:UniProtKB-KW
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of translationISS:UniProtKB
Negative regulation of viral genome replicationISS:UniProtKB
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Nucleus, nucleolus; Cytoplasm; Nucleus. Localizes in the cytoplasm in response to viral infection. The unphosphorylated form is retained in the nucleus by ILF2. Phosphorylation at Thr-188 and Thr-315 causes the dissociation of ILF2 from the ILF2-ILF3 complex resulting in a cytoplasmic sequestration of ILF3. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

PTM

Phosphorylated at Thr-188 and Thr-315 by PKR in response to certain RNA viruses. This phosphorylation results in the dissociation of ILF2 from the ILF2-ILF3 complex resulting in a cytoplasmic sequestration of ILF3 where it can bind to viral RNAs and impede viral replication.
Methylated by protein arginine N-methyltransferase 1.
Ubiquitinated at Lys-297 in a TRIM47-dependent manner; this 'Lys-48'-linked ubiquitination promotes ILF3 degradation.

Qiu, J., Wu, X., Luo, Y., Yao, Y., Zhang, X., Pan, B., ... & Tang, N. (2023). Prognostic and immunotherapeutic predictive value of interleukin enhancer-binding factor 3 in hepatocellular carcinoma: Integrated bioinformatics and experimental analysis. Gene, 856, 147132.

Li, P., Mi, Q., Yan, S., Xie, Y., Cui, Z., Zhang, S., ... & Wang, C. (2023). Characterization of circSCL38A1 as a novel oncogene in bladder cancer via targeting ILF3/TGF-β2 signaling axis. Cell Death & Disease, 14(1), 59.

Huang, Y., Zheng, Y., Yao, L., Qiao, F., Hou, Y., Hu, X., ... & Shao, Z. (2022). RNA binding protein POP7 regulates ILF3 mRNA stability and expression to promote breast cancer progression. Cancer Science, 113(11), 3801-3813.

Xu, Z., Huang, H., Li, X., Ji, C., Liu, Y., Liu, X., ... & Shi, J. (2020). High expression of interleukin‑enhancer binding factor 3 predicts poor prognosis in patients with lung adenocarcinoma. Oncology Letters, 19(3), 2141-2152.

Liu, Y., Li, Y., Zhao, Y., Liu, Y., Fan, L., Jia, N., & Zhao, Q. (2019). ILF3 promotes gastric cancer proliferation and may be used as a prognostic marker. Molecular Medicine Reports, 20(1), 125-134.

Jia, R., Ajiro, M., Yu, L., McCoy, P., & Zheng, Z. M. (2019). Oncogenic splicing factor SRSF3 regulates ILF3 alternative splicing to promote cancer cell proliferation and transformation. Rna, 25(5), 630-644.

Vrakas, C. N., Herman, A. B., Ray, M., Kelemen, S. E., Scalia, R., & Autieri, M. V. (2019). RNA stability protein ILF3 mediates cytokine-induced angiogenesis. The FASEB Journal, 33(3), 3304.

Cheng, C. C., Chou, K. F., Wu, C. W., Su, N. W., Peng, C. L., Su, Y. W., ... & Chang, Y. F. (2018). EGFR-mediated interleukin enhancer-binding factor 3 contributes to formation and survival of cancer stem-like tumorspheres as a therapeutic target against EGFR-positive non-small cell lung cancer. Lung Cancer, 116, 80-89.

Wu, T. H., Shi, L., Adrian, J., Shi, M., Nair, R. V., Snyder, M. P., & Kao, P. N. (2018). NF90/ILF3 is a transcription factor that promotes proliferation over differentiation by hierarchical regulation in K562 erythroleukemia cells. PloS one, 13(3), e0193126.

Cheng, C. C., Chou, K. F., Wu, C. W., Su, N. W., Peng, C. L., Su, Y. W., ... & Chang, Y. F. (2018). EGFR-mediated interleukin enhancer-binding factor 3 contributes to formation and survival of cancer stem-like tumorspheres through regulating HER3/ERBB3 expression as a therapeutic target against EGFR-positive non-small cell lung cancer. Cancer Research, 78(13_Supplement), 3060-3060.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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