Mouse Anti-IMPG2 Recombinant Antibody (3H5) (CBMAB-A4456-LY)

Name: Mouse Anti-IMPG2 Recombinant Antibody (3H5)
SKU: CBMAB-A4456-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3H5

Application

WB, ELISA

Immunogen

IMPG2 (NP_057331.2, 572 a.a. ~ 678 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

interphotoreceptor matrix proteoglycan 2

Introduction

Interphotoreceptor matrix proteoglycan-2 is part of an extracellular complex occupying the interface between photoreceptors and the retinal pigment epithelium in the fundus of the eye.[supplied by OMIM

Entrez Gene ID

50939

UniProt ID

Q9BZV3

Alternative Names

IPM200; SPACRCAN

Function

Chondroitin sulfate- and hyaluronan-binding proteoglycan involved in the organization of interphotoreceptor matrix; may participate in the maturation and maintenance of the light-sensitive photoreceptor outer segment. Binds heparin.

Biological Process

Extracellular matrix organizationIEA:Ensembl
Protein localizationIEA:Ensembl
Retina morphogenesis in camera-type eyeIEA:Ensembl
Visual perceptionManual Assertion Based On ExperimentTAS:UniProtKB

Cellular Location

Photoreceptor outer segment membrane; Photoreceptor inner segment membrane; Secreted, extracellular space, extracellular matrix, interphotoreceptor matrix

Involvement in disease

Retinitis pigmentosa 56 (RP56):
A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Macular dystrophy, vitelliform, 5 (VMD5):
A form of macular dystrophy, a retinal disease in which various forms of deposits, pigmentary changes, and atrophic lesions are observed in the macula lutea. Vitelliform macular dystrophies are characterized by yellow, lipofuscin-containing deposits, usually localized at the center of the macula. VMD5 features include late-onset moderate visual impairment and preservation of retinal pigment epithelium reflectivity.

Topology

Extracellular: 23-1099
Helical: 1100-1120
Cytoplasmic: 1121-1241

PTM

Highly glycosylated (N- and O-linked carbohydrates).

Castellini, M. E., Spagnolli, G., Poggi, L., Biasini, E., Casarosa, S., & Messina, A. (2023). Identification of the zebrafish homologues of IMPG2, a retinal proteoglycan. Cell and Tissue Research, 1-13.

Mayerl, S. J., Bajgai, S., Ludwig, A. L., Jager, L. D., Williams, B. N., Bacig, C., ... & Gamm, D. M. (2022). Human retinal organoids harboring IMPG2 mutations exhibit a photoreceptor outer segment phenotype that models advanced retinitis pigmentosa. Stem Cell Reports, 17(11), 2409-2420.

Vázquez-Domínguez, I., Li, C. H., Fadaie, Z., Haer-Wigman, L., Cremers, F. P., Garanto, A., ... & Roosing, S. (2022). Identification of a complex allele in IMPG2 as a cause of adult-onset vitelliform macular dystrophy. Investigative Ophthalmology & Visual Science, 63(5), 27-27.

Mitchell, B., Coulter, C., Geldenhuys, W. J., Rhodes, S., & Salido, E. M. (2022). Interphotoreceptor matrix proteoglycans IMPG1 and IMPG2 proteolyze in the SEA domain and reveal localization mutual dependency. Scientific Reports, 12(1), 15535.

Chatterjee, S., Gupta, S., Chaudhry, V. N., Chaudhry, P., Mukherjee, A., & Mutsuddi, M. (2022). Whole exome sequencing identifies a novel splice-site mutation in IMPG2 gene causing Stargardt-like juvenile macular dystrophy in a north Indian family. Gene, 816, 146158.

Shah, S. M., Schimmenti, L. A., Marmorstein, A. D., & Bakri, S. J. (2021). Adult-onset vitelliform macular dystrophy secondary to a novel IMPG2 gene variant. Retinal Cases and Brief Reports, 15(4), 356-358.

Hitti-Malin, R. J., Burmeister, L. M., Ricketts, S. L., Lewis, T. W., Pettitt, L., Boursnell, M., ... & Mellersh, C. S. (2020). A LINE-1 insertion situated in the promoter of IMPG2 is associated with autosomal recessive progressive retinal atrophy in Lhasa Apso dogs. BMC genetics, 21(1), 1-17.

Salido, E. M., & Ramamurthy, V. (2020). Proteoglycan IMPG2 shapes the interphotoreceptor matrix and modulates vision. Journal of Neuroscience, 40(20), 4059-4072.

Xu, H., Qu, C., Gan, L., Sun, K., Tan, J., Liu, X., ... & Zhang, L. (2020). Deletion of the Impg2 gene causes the degeneration of rod and cone cells in mice. Human Molecular Genetics, 29(10), 1624-1634.

Khan, A. O., & Al Teneiji, A. M. (2019). Homozygous and heterozygous retinal phenotypes in families harbouring IMPG2 mutations. Ophthalmic genetics, 40(3), 247-251.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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