Mouse Anti-INTS6 Recombinant Antibody (3D9) (CBMAB-A2159-LY)

Name: Mouse Anti-INTS6 Recombinant Antibody (3D9)
SKU: CBMAB-A2159-LY
Rating: 5 (1 reviews)

See all INTS6 antibodies

Online Inquiry

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3D9

Antibody Isotype

IgG1, κ

Application

WB, ELISA

Basic Information

Immunogen

DDX26 (NP_036273, 779 a.a. ~ 887 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

integrator complex subunit 6

Introduction

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. The protein encoded by this gene is a DEAD box protein that is part of a complex that interacts with the C-terminus of RNA polymerase II and is involved in 3' end processing of snRNAs. In addition, this gene is a candidate tumor suppressor and located in the critical region of loss of heterozygosity (LOH). Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Entrez Gene ID

26512

UniProt ID

Q9UL03

Alternative Names

DBI-1; DDX26; DDX26A; DICE1; DKFZp434B105; HDB; INT6; Notchl2

Function

Component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes (Probable). Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex (PubMed:23904267).
May have a tumor suppressor role; an ectopic expression suppressing tumor cell growth (PubMed:15254679, PubMed:16239144).

Biological Process

snRNA 3'-end processingManual Assertion Based On ExperimentIBA:GO_Central
snRNA processingManual Assertion Based On ExperimentIDA:HGNC-UCL

Cellular Location

Nucleus

More Infomation

References

Fujiwara, R., Zhai, S. N., Liang, D., Tracey, M., Ma, X. K., Shah, A. P., ... & Wilusz, J. E. (2023). IntS6 and the Integrator phosphatase module tune the efficiency of select premature transcription termination events. bioRxiv, 2023-03.

Jia, Y., Cheng, Z., Bharath, S. R., Sun, Q., Su, N., Huang, J., & Song, H. (2021). Crystal structure of the INTS3/INTS6 complex reveals the functional importance of INTS3 dimerization in DSB repair. Cell Discovery, 7(1), 66.

Ding, X., Chen, T., Shi, Q., Nan, P., Wang, X., Xie, D., & Li, J. (2021). INTS6 promotes colorectal cancer progression by activating of AKT and ERK signaling. Experimental Cell Research, 407(2), 112826.

Chen, H., Shen, H. X., Lin, Y. W., Mao, Y. Q., Liu, B., & Xie, L. P. (2018). Small RNA-induced Ints6 gene up-regulation suppresses castration-resistant prostate cancer cells by regulating Β-catenin signaling. Cell Cycle, 17(13), 1602-1613.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-INTS6 Recombinant Antibody (3D9)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: