Mouse Anti-IPO5 Recombinant Antibody (N9C1) (CBMAB-P0405-YC)

Name: Mouse Anti-IPO5 Recombinant Antibody (N9C1)
SKU: CBMAB-P0405-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

N9C1

Application

EMSA, IP, WB

Immunogen

Proximal sequence element (PSE1) of the human U1 small nuclear RNA extracted from human placenta

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS with 0.02% sodium azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Importin 5

Introduction

Nucleocytoplasmic transport, a signal-and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Entrez Gene ID

3843

UniProt ID

O00410

Alternative Names

RANBP5; KPNB3; Pse1; imp5; IMB3

Function

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity).
Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Binds to CPEB3 and mediates its nuclear import following neuronal stimulation (By similarity).
In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.

Biological Process

Cellular response to amino acid stimulusISS:UniProtKB
Negative regulation of cyclin-dependent protein serine/threonine kinase activityManual Assertion Based On ExperimentTAS:ARUK-UCL
NLS-bearing protein import into nucleusISS:UniProtKB
Positive regulation of protein import into nucleusISS:UniProtKB
Protein import into nucleusManual Assertion Based On ExperimentIBA:GO_Central
Ribosomal protein import into nucleusManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasm; Nucleus; Nucleus, nucleolus. Nucleus; nuclear rim. Found particularly in the nuclear rim and nucleolus.

Radhakrishnan, K., Luu, M., Iaria, J., Sutherland, J. M., McLaughlin, E. A., Zhu, H. J., & Loveland, K. L. (2023). Activin and BMP Signalling in Human Testicular Cancer Cell Lines, and a Role for the Nucleocytoplasmic Transport Protein Importin-5 in Their Crosstalk. Cells, 12(7), 1000.

Panagiotopoulos, A. A., Kalyvianaki, K., Tsodoulou, P. K., Darivianaki, M. N., Dellis, D., Notas, G., ... & Kampa, M. (2022). Recognition motifs for importin 4 [(L) PPRS (G/P) P] and importin 5 [KP (K/Y) LV] binding, identified by bio-informatic simulation and experimental in vitro validation. Computational and Structural Biotechnology Journal, 20, 5952-5961.

Lin, C., Hu, J., Dai, Y., Zhang, H., Xu, K., Dong, W., ... & Gu, J. (2022). Porcine circovirus type 2 hijacks host IPO5 to sustain the intracytoplasmic stability of its capsid protein. Journal of Virology, 96(23), e01522-22.

Patouret, R. (2021). The nuclear transport protein importin-5: A promising target in oncology and virology. Chimia, 75(4), 319-319.

Liu, W., Patouret, R., Barluenga, S., Plank, M., Loewith, R., & Winssinger, N. (2021). Identification of a covalent importin-5 inhibitor, goyazensolide, from a collective synthesis of furanoheliangolides. ACS Central Science, 7(6), 954-962.

Zhang, W., Lu, Y., Li, X., Zhang, J., Lin, W., Zhang, W., ... & Li, X. (2019). IPO5 promotes the proliferation and tumourigenicity of colorectal cancer cells by mediating RASAL2 nuclear transportation. Journal of Experimental & Clinical Cancer Research, 38, 1-14.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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