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Rat Anti-Itga4 Recombinant Antibody (CBYJL-2431) (CBMAB-L2766-YJ)

Provided herein is a Rat monoclonal antibody, which binds to Integrin Alpha 4 (Itga4). The antibody can be used for immunoassay techniques, such as Bioimaging, FC, IHC-Fr, IP.
See all Itga4 antibodies

Summary

Host Animal
Rat
Specificity
Mouse
Clone
CBYJL-2431
Antibody Isotype
IgG2
Application
Bioimaging, FC, IHC-Fr, IP

Basic Information

Immunogen
TK1 cells.
Specificity
Mouse
Antibody Isotype
IgG2
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Integrin Subunit Alpha 4
Introduction
Integrins alpha-4/beta-1 (VLA-4 or LPAM-2) and alpha-4/beta-7 (LPAM-1) are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1.
Entrez Gene ID
UniProt ID
Alternative Names
CD49D; Itga4B; integrin alpha-4; CD49 antigen-like family member D; LPAM subunit alpha; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha; integrin alpha-IV; integrin alpha4B; lymphocyte Peyer patch adhesion molecules subunit alpha
Function
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415).
ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877).
Biological Process
Axonogenesis involved in innervationIEA:Ensembl
B cell differentiation1 PublicationIC:BHF-UCL
Cell-cell adhesion in response to extracellular stimulusManual Assertion Based On ExperimentIGI:ARUK-UCL
Cell-cell adhesion mediated by integrinManual Assertion Based On ExperimentIGI:ARUK-UCL
Cell-matrix adhesionManual Assertion Based On ExperimentIMP:UniProtKB
Cell-matrix adhesion involved in ameboidal cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Cellular response to amyloid-betaManual Assertion Based On ExperimentIGI:ARUK-UCL
Cellular response to cytokine stimulusIEA:Ensembl
Clathrin-dependent extracellular exosome endocytosisIEA:Ensembl
DiapedesisIEA:Ensembl
Endodermal cell differentiationManual Assertion Based On ExperimentIEP:UniProtKB
Heterotypic cell-cell adhesionManual Assertion Based On ExperimentIMP:UniProtKB
Import into cellIEA:Ensembl
Integrin-mediated signaling pathwayIEA:UniProtKB-KW
Leukocyte cell-cell adhesionManual Assertion Based On ExperimentIDA:BHF-UCL
Leukocyte tethering or rollingManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of protein homodimerization activityManual Assertion Based On ExperimentIDA:UniProtKB
Neuron projection extensionIEA:Ensembl
Positive regulation of endothelial cell apoptotic processManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of leukocyte tethering or rollingIEA:Ensembl
Positive regulation of T cell migrationIEA:Ensembl
Positive regulation of vascular endothelial cell proliferationManual Assertion Based On ExperimentIMP:BHF-UCL
Receptor clusteringManual Assertion Based On ExperimentIMP:UniProtKB
Substrate adhesion-dependent cell spreadingManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Membrane
Topology
Extracellular: 35-977
Helical: 978-1001
Cytoplasmic: 1002-1032
PTM
Phosphorylation on Ser-1027 inhibits PXN binding.

Fang, T., Yin, X., Wang, Y., Wang, H., Wang, X., & Xue, Y. (2022). Lymph node metastasis-related gene ITGA4 promotes the proliferation, migration, and invasion of gastric cancer cells by regulating tumor immune microenvironment. Journal of Oncology, 2022.

Li, M., Ye, J., Xia, Y., Li, M., Li, G., Hu, X., ... & Ji, C. (2022). METTL3 mediates chemoresistance by enhancing AML homing and engraftment via ITGA4. Leukemia, 36(11), 2586-2595.

Jafarpour, S., Saberi, F., Yazdi, M., Nedaeinia, R., Amini, G., Ferns, G. A., & Salehi, R. (2022). Association between colorectal cancer and the degree of ITGA4 promoter methylation in peripheral blood mononuclear cells. Gene Reports, 27, 101580.

Mo, J., Zhang, J., Huang, H., Liu, C., Cheng, Y., Mo, Y., ... & Zhang, B. (2022). The early predictive effect of low expression of the ITGA4 in colorectal cancer. Journal of Gastrointestinal Oncology, 13(1), 265.

Attia, H. R., Ibrahim, M. H., El-Aziz, S. H. A., Hassan, N. M., Osman, R. A., Hagag, H. A., ... & Sobeih, M. E. (2020). ITGA4 gene methylation status in chronic lymphocytic leukemia. Future science OA, 6(7), FSO583.

Yan, J., Yang, X., Jiao, X., Yang, X., Guo, M., Chen, Y., ... & Chen, W. (2020). Integrative transcriptomic and proteomic analysis reveals CD9/ITGA4/PI3K‐Akt axis mediates trabecular meshwork cell apoptosis in human glaucoma. Journal of Cellular and Molecular Medicine, 24(1), 814-829.

Durmanova, V., Parnicka, Z., Javor, J., Minarik, G., Vrazda, L., Vaseckova, B., ... & Shawkatova, I. (2018). A novel association of polymorphism in the ITGA4 gene encoding the VLA-4 α4 subunit with increased risk of Alzheimer’s disease. Mediators of Inflammation, 2018.

Mostafavi-Pour, Z., Ashrafi, M. R., & Talaei-Khozani, T. (2018). Down regulation of ITGA4 and ITGA5 genes after formation of 3D spherules by human Wharton’s jelly stem cells (hWJSCs). Molecular biology reports, 45, 245-252.

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For research use only. Not intended for any clinical use.

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