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Mouse Anti-JMJD7 Recombinant Antibody (1G5) (CBMAB-0103-WJ)

This product is a mouse antibody that recognizes JMJD7. The antibody 1G5 can be used for immunoassay techniques such as: ELISA, WB.
See all JMJD7 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1G5
Antibody Isotype
IgG2a, Κ
Application
ELISA, WB

Basic Information

Immunogen
Full-length recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG2a, Κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
jumonji domain containing 7
Introduction
JMJD7 (Jumonji Domain Containing 7) encodes a highly conserved protein with a JmjC domain, which are part of the cupin metalloenzyme superfamily. JmjC proteins may function as 2-oxoglutarate-Fe(II)-dependent dioxygenases.
Entrez Gene ID
100137047
UniProt ID
P0C870
Function
Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monooxygenase (PubMed:28847961, PubMed:29915238).
Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation (PubMed:28847961).
Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4 (PubMed:28847961).
After initial cleavage, continues to digest histones tails via its aminopeptidase activity (PubMed:28847961).
Additionally, may play a role in protein biosynthesis by modifying the translation machinery (PubMed:29915238).
Acts as Fe(2+) and 2-oxoglutarate-dependent monooxygenase, catalyzing (S)-stereospecific hydroxylation at C-3 of 'Lys-22' of DRG1 and 'Lys-21' of DRG2 translation factors (TRAFAC), promoting their interaction with ribonucleic acids (RNA) (PubMed:29915238).
Biological Process
Protein hydroxylationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus; Cytoplasm

Liu, H., Wei, P., Zhang, Q., Chen, Z., Liu, J., & Zhang, G. (2022). The Novel Protease Activities of JMJD5–JMJD6–JMJD7 and Arginine Methylation Activities of Arginine Methyltransferases Are Likely Coupled. Biomolecules, 12(3), 347.

Chowdhury, R., Abboud, M. I., Wiley, J., Tumber, A., Markolovic, S., & Schofield, C. J. (2022). Conservation of the unusual dimeric JmjC fold of JMJD7 from Drosophila melanogaster to humans. Scientific Reports, 12(1), 6065.

Zhang, W., Li, K., Wang, T., Wu, M., & Li, L. (2021). Discovery of JMJD7 inhibitors with the aid of virtual screening and bioactivity evaluation. Bioorganic & Medicinal Chemistry Letters, 45, 128139.

Liu, Y., Arai, A., Kim, T., Kim, S., Park, N. H., & Kim, R. H. (2018). Jmjd7 negatively regulates osteoclast differentiation. The Chinese journal of dental research: the official journal of the Scientific Section of the Chinese Stomatological Association (CSA), 21(2), 113.

Yingci, L. I. U., Atsushi, A. R. A. I., Terresa, K. I. M., Sol, K. I. M., No-Hee, P. A. R. K., & Kim, R. H. (2018). Histone Demethylase Jmjd7 negatively regulates differentiation of Osteoclast. Chinese Journal of Dental Research, 21(2).

Markolovic, S., Zhuang, Q., Wilkins, S. E., Eaton, C. D., Abboud, M. I., Katz, M. J., ... & Schofield, C. J. (2018). The Jumonji-C oxygenase JMJD7 catalyzes (3 S)-lysyl hydroxylation of TRAFAC GTPases. Nature chemical biology, 14(7), 688-695.

Liu, H., Wang, C., Lee, S., Ning, F., Wang, Y., Zhang, Q., ... & Zhang, G. (2018). Specific recognition of arginine methylated histone tails by JMJD5 and JMJD7. Scientific reports, 8(1), 3275.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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