Mouse Anti-JMJD7 Recombinant Antibody (1G5) (CBMAB-0103-WJ)

Name: Mouse Anti-JMJD7 Recombinant Antibody (1G5)
SKU: CBMAB-0103-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1G5

Application

ELISA, WB

Immunogen

Full-length recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, Κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

jumonji domain containing 7

Introduction

JMJD7 (Jumonji Domain Containing 7) encodes a highly conserved protein with a JmjC domain, which are part of the cupin metalloenzyme superfamily. JmjC proteins may function as 2-oxoglutarate-Fe(II)-dependent dioxygenases.

Entrez Gene ID

100137047

UniProt ID

P0C870

Function

Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monooxygenase (PubMed:28847961, PubMed:29915238).
Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation (PubMed:28847961).
Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4 (PubMed:28847961).
After initial cleavage, continues to digest histones tails via its aminopeptidase activity (PubMed:28847961).
Additionally, may play a role in protein biosynthesis by modifying the translation machinery (PubMed:29915238).
Acts as Fe(2+) and 2-oxoglutarate-dependent monooxygenase, catalyzing (S)-stereospecific hydroxylation at C-3 of 'Lys-22' of DRG1 and 'Lys-21' of DRG2 translation factors (TRAFAC), promoting their interaction with ribonucleic acids (RNA) (PubMed:29915238).

Biological Process

Protein hydroxylationManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Nucleus; Cytoplasm

Liu, H., Wei, P., Zhang, Q., Chen, Z., Liu, J., & Zhang, G. (2022). The Novel Protease Activities of JMJD5–JMJD6–JMJD7 and Arginine Methylation Activities of Arginine Methyltransferases Are Likely Coupled. Biomolecules, 12(3), 347.

Chowdhury, R., Abboud, M. I., Wiley, J., Tumber, A., Markolovic, S., & Schofield, C. J. (2022). Conservation of the unusual dimeric JmjC fold of JMJD7 from Drosophila melanogaster to humans. Scientific Reports, 12(1), 6065.

Zhang, W., Li, K., Wang, T., Wu, M., & Li, L. (2021). Discovery of JMJD7 inhibitors with the aid of virtual screening and bioactivity evaluation. Bioorganic & Medicinal Chemistry Letters, 45, 128139.

Liu, Y., Arai, A., Kim, T., Kim, S., Park, N. H., & Kim, R. H. (2018). Jmjd7 negatively regulates osteoclast differentiation. The Chinese journal of dental research: the official journal of the Scientific Section of the Chinese Stomatological Association (CSA), 21(2), 113.

Yingci, L. I. U., Atsushi, A. R. A. I., Terresa, K. I. M., Sol, K. I. M., No-Hee, P. A. R. K., & Kim, R. H. (2018). Histone Demethylase Jmjd7 negatively regulates differentiation of Osteoclast. Chinese Journal of Dental Research, 21(2).

Markolovic, S., Zhuang, Q., Wilkins, S. E., Eaton, C. D., Abboud, M. I., Katz, M. J., ... & Schofield, C. J. (2018). The Jumonji-C oxygenase JMJD7 catalyzes (3 S)-lysyl hydroxylation of TRAFAC GTPases. Nature chemical biology, 14(7), 688-695.

Liu, H., Wang, C., Lee, S., Ning, F., Wang, Y., Zhang, Q., ... & Zhang, G. (2018). Specific recognition of arginine methylated histone tails by JMJD5 and JMJD7. Scientific reports, 8(1), 3275.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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