Rabbit Anti-KANK2 Recombinant Antibody (EG1735) (CBMAB-EN2073-LY)

The product is antibody recognizes KANK2. The antibody EG1735 immunoassay techniques such as: IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000.
See all KANK2 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human

Clone

EG1735

Antibody Isotype

IgG

Application

IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

Basic Information

Immunogen

The antibody was produced against synthesized peptide derived from internal of human KANK2.

Specificity

Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

KN Motif And Ankyrin Repeat Domains 2

Introduction

This gene encodes a member of the KN motif and ankyrin repeat domains (KANK) family of proteins, which play a role in cytoskeletal formation by regulating actin polymerization. The encoded protein functions in the sequestration of steroid receptor coactivators and possibly other proteins. Mutations in this gene are associated with impaired kidney podocyte function and nephrotic syndrome, and keratoderma and woolly hair. [provided by RefSeq, Jul 2016]

Entrez Gene ID

25959

UniProt ID

Q63ZY3

Alternative Names

KN Motif And Ankyrin Repeat Domains 2; Ankyrin Repeat Domain-Containing Protein 25; Matrix-Remodeling-Associated Protein 3; SRC1-Interacting Protein; SRC-Interacting Protein; ANKRD25; MXRA3; SIP; KN Motif And Ankyrin Repeat Domain-Containing Protein 2;

Function

Involved in transcription regulation by sequestering in the cytoplasm nuclear receptor coactivators such as NCOA1, NCOA2 and NCOA3 (PubMed:17476305).
Involved in regulation of caspase-independent apoptosis by sequestering the proapoptotic factor AIFM1 in mitochondria (PubMed:22371500).
Pro-apoptotic stimuli can induce its proteasomal degradation allowing the translocation of AIFM1 to the nucleus to induce apoptosis (PubMed:22371500).
Involved in the negative control of vitamin D receptor signaling pathway (PubMed:24671081).
Involved in actin stress fibers formation through its interaction with ARHGDIA and the regulation of the Rho signaling pathway (PubMed:17996375, PubMed:25961457).
May thereby play a role in cell adhesion and migration, regulating for instance podocytes migration during development of the kidney (PubMed:25961457).
Through the Rho signaling pathway may also regulate cell proliferation (By similarity).

Biological Process

Apoptotic processIEA:UniProtKB-KW
Glomerular visceral epithelial cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Kidney epithelium developmentManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of cell population proliferationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of intracellular estrogen receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of programmed cell deathManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of vitamin D receptor signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of Rho protein signal transductionManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cytoplasm; Mitochondrion

Involvement in disease

Palmoplantar keratoderma and woolly hair (PPKWH):
A disorder characterized by abnormal thickening of the skin on the palms and soles, in association with woolly scalp hair. Affected individuals manifest a variable degree of striate palmoplantar keratoderma, generally more severe on the soles. Leukonychia is more pronounced on the fingernails than toenails. Scalp hair, body hair, eyebrows, and eyelashes are sparse. The fifth toes show variable degrees of pseudoainhum, ranging from external rotation to a deep sulcus at the digitoplantar fold, accompanied by a bulbous appearance of the distal toe.
Nephrotic syndrome 16 (NPHS16):
A form of nephrotic syndrome, a renal disease clinically characterized by severe proteinuria, resulting in complications such as hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form that progresses to end-stage renal failure. NPHS16 inheritance is autosomal recessive.

PTM

Phosphorylated by casein kinase II upon estrogen stimulation (PubMed:17476305).
Phosphorylation induces the release by KANK2 of NCOA1 and its translocation to the nucleus where NCOA1 can activate gene transcription (PubMed:17476305).

References

Lončarić, M., Stojanović, N., Rac-Justament, A., Coopmans, K., Majhen, D., Humphries, J. D., ... & Ambriović-Ristov, A. (2023). Talin2 and KANK2 functionally interact to regulate microtubule dynamics, paclitaxel sensitivity and cell migration in the MDA-MB-435S melanoma cell line. Cellular & Molecular Biology Letters, 28(1), 56.

Pei, Q., Ni, W., Yuan, Y., Yuan, J., Zhang, X., & Yao, M. (2022). HSP70 ameliorates septic lung injury via inhibition of apoptosis by interacting with KANK2. Biomolecules, 12(3), 410.

Xu, Y., Guo, C., Pan, W., Zhao, C., Ding, Y., Xie, X., ... & Yu, C. (2021). Nephrotic-syndrome-associated mutation of KANK2 induces pathologic binding competition with physiological interactor KIF21A. Journal of Biological Chemistry, 297(2).

Paradžik, M., Humphries, J. D., Stojanović, N., Nestić, D., Majhen, D., Dekanić, A., ... & Ambriović-Ristov, A. (2020). KANK2 links αVβ5 focal adhesions to microtubules and regulates sensitivity to microtubule poisons and cell migration. Frontiers in cell and developmental biology, 8, 125.

Li, L., Li, Z. B., Jia, M., & Chu, H. T. (2020). Therapeutic effects of KANK2 in myocardial infarction rats might be associated with the NF-κB p65 inhibition. International Immunopharmacology, 86, 106687.

Guo, Q., Liao, S., Zhu, Z., Li, Y., Li, F., & Xu, C. (2018). Structural basis for the recognition of kinesin family member 21A (KIF21A) by the ankyrin domains of KANK1 and KANK2 proteins. Journal of Biological Chemistry, 293(2), 557-566.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

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