Rabbit Anti-KATNA1 Recombinant Antibody (CBLY1-040) (CBMAB-K0440-LY)

This product is antibody recognizes KATNA1. The antibody CBLY1-040 immunoassay techniques such as: WB.
See all KATNA1 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human

Clone

CBLY1-040

Antibody Isotype

IgG

Application

Basic Information

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Katanin Catalytic Subunit A1

Introduction

Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. This gene encodes the p80 subunit. This protein is a member of the AAA family of ATPases. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2011]

Entrez Gene ID

11104

UniProt ID

O75449

Alternative Names

Katanin Catalytic Subunit A1; EC 3.6.4.3; Katanin P60 (ATPase-Containing) Subunit A 1; Katanin P60 (ATPase Containing) Subunit A 1; Katanin P60 Subunit A1; P60 Katanin;

Function

Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.

Biological Process

Cell cycleIEA:UniProtKB-KW
Cell divisionIEA:UniProtKB-KW
Microtubule severingManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm; Midbody; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle pole; Cytoplasm, cytoskeleton, spindle. Predominantly cytoplasmic (PubMed:9658175).
Localized diffusely in the cytoplasm during the interphase (PubMed:10751153).
During metaphase is localized throughout the cell and more widely dispersed than the microtubules. In anaphase and telophase is localized at the midbody region (PubMed:19261606).
Also localized to the interphase centrosome and the mitotic spindle poles (By similarity).
Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1 (PubMed:10751153).
Localizes within the cytoplasm, partially overlapping with microtubules, in interphase and to the mitotic spindle and spindle poles during mitosis (PubMed:26929214).

PTM

Phosphorylation by DYRK2 triggers ubiquitination and subsequent degradation.
Ubiquitinated by the BCR(KLHL42) E3 ubiquitin ligase complex, leading to its proteasomal degradation. Ubiquitinated by the EDVP E3 ligase complex and subsequently targeted for proteasomal degradation.

References

Yuen, W. S., Zhang, Q. H., Dunstan, M., Aidhikari, D., O’Connor, A. E., Dunleavy, J. E., ... & Carroll, J. (2023). Fertility is compromised after oocyte-specific deletion of Katanin A-subunit, Katna1, but not Katnal1. bioRxiv, 2023-10.

Dunleavy, J. E., Graffeo, M., Wozniak, K., O'Connor, A. E., Merriner, D. J., Nguyen, J., ... & O'Bryan, M. K. (2023). Katanin A-subunits KATNA1 and KATNAL1 act co-operatively in mammalian meiosis and spermiogenesis to achieve male fertility. Development, dev-201956.

Li, S., Liang, Y., Zou, J., Cai, Z., Yang, H., Yang, J., ... & Tan, M. (2022). SUMOylation of microtubule-cleaving enzyme KATNA1 promotes microtubule severing and neurite outgrowth. Journal of Biological Chemistry, 298(9).

Dunleavy, J. E., Graffeo, M., Wozniak, K., O’Connor, A. E., Merriner, D. J., Nguyen, J., ... & O’Bryan, M. K. (2022). Male mammalian meiosis and spermiogenesis is critically dependent on the shared functions of the katanins KATNA1 and KATNAL1. bioRxiv, 2022-11.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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