Mouse Anti-Kcnk18 Recombinant Antibody (CBYJT-4632) (CBMAB-T4162-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Kcnk18 (Potassium Channel, Subfamily K, Member 18). The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all Kcnk18 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Mouse

Clone

CBYJT-4632

Antibody Isotype

IgG

Application

WB, IP, IF, ELISA

Basic Information

Specificity

Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Potassium Channel, Subfamily K, Member 18

Introduction

Kcnk18 has several functions. Outward rectifying potassium channel. Produces rapidly activating outward rectifier K+ currents. may play a role as background potassium channel that sets the resting membrane potential. Channel activity is directly activated by calcium signal. Activated by the G(q)-protein coupled receptor pathway. The calcium signal robustly activates the channel via calcineurin, whereas the anchoring of 14-3-3/YWHAH interferes with the return of the current to the resting state after activation. Inhibited also by arachidonic acid and other naturally occurring unsaturated free fatty acids. Channel activity is also enhanced by volatile anesthetics, such as isoflurane. Appears to be the primary target of hydroxy-alpha-sanshool, an ingredient of Schezuan pepper. may be involved in the somatosensory function with special respect to pain sensation.

Entrez Gene ID

332396

UniProt ID

Q6VV64

Alternative Names

Trik; Gm781; Tresk; Tresk-2

Function

Outward rectifying potassium channel. Produces rapidly activating outward rectifier K(+) currents. May function as background potassium channel that sets the resting membrane potential. Channel activity is directly activated by calcium signal. Activated by the G(q)-protein coupled receptor pathway. The calcium signal robustly activates the channel via calcineurin, whereas the anchoring of 14-3-3/YWHAH interferes with the return of the current to the resting state after activation. Inhibited also by arachidonic acid and other naturally occurring unsaturated free fatty acids. Channel activity is also enhanced by volatile anesthetics, such as isoflurane. Appears to be the primary target of hydroxy-alpha-sanshool, an ingredient of Schezuan pepper. May be involved in the somatosensory function with special respect to pain sensation (By similarity).

Biological Process

Cellular response to pHManual Assertion Based On ExperimentIDA:BHF-UCL
Potassium ion export across plasma membraneManual Assertion Based On ExperimentIDA:BHF-UCL
Potassium ion transmembrane transportManual Assertion Based On ExperimentIBA:GO_Central
Potassium ion transportManual Assertion Based On ExperimentIDA:UniProtKB
Stabilization of membrane potentialManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cell membrane

Involvement in disease

Migraine with or without aura 13 (MGR13):
A form of migraine transmitted in an autosomal dominant pattern. Migraine is a disabling symptom complex of periodic headaches, usually temporal and unilateral. Headaches are often accompanied by irritability, nausea, vomiting and photophobia, preceded by constriction of the cranial arteries. The two major subtypes are common migraine (migraine without aura) and classic migraine (migraine with aura). Classic migraine is characterized by recurrent attacks of reversible neurological symptoms (aura) that precede or accompany the headache. Aura may include a combination of sensory disturbances, such as blurred vision, hallucinations, vertigo, numbness and difficulty in concentrating and speaking.

Topology

Cytoplasmic: 1-23
Helical: 24-44
Pore-forming: 103-129
Helical: 130-148
Cytoplasmic: 149-280
Helical: 281-301
Pore-forming: 314-328
Helical: 335-355
Cytoplasmic: 356-384

PTM

Phosphorylation of Ser-252 is required for the binding of 14-3-3eta/YWHAH. Calcineurin-mediated dephosphorylation of Ser-264 enhances channel activity (By similarity).
N-glycosylated.

References

Majethia, P., Harish, R., Narayanan, D. L., Yatheesha, B. L., Sharma, S., & Shukla, A. (2023). Further evidence of biallelic variants in KCNK18 as a cause of intellectual disability and epilepsy with febrile seizure plus. Clinical Dysmorphology, 32(4), 147-150.

Yin, C., Zeng, F., Huang, P., Shi, Z., Yang, Q., Pei, Z., ... & Wang, Y. (2023). The Bi-Functional Paxilline Enriched in Skin Secretion of Tree Frogs (Hyla japonica) Targets the KCNK18 and BKCa Channels. Toxins, 15(1), 70.

Pavinato, L., Nematian-Ardestani, E., Zonta, A., De Rubeis, S., Buxbaum, J., Mancini, C., ... & Brusco, A. (2021). KCNK18 biallelic variants associated with intellectual disability and neurodevelopmental disorders alter TRESK channel activity. International journal of molecular sciences, 22(11), 6064.

Kowalska, M., Prendecki, M., Kapelusiak-Pielok, M., Grzelak, T., Łagan-Jędrzejczyk, U., Wiszniewska, M., ... & Dorszewska, J. (2020). Analysis of Genetic Variants in SCN1A, SCN2A, KCNK18, TRPA1 and STX1A as a Possible Marker of Migraine. Current Genomics, 21(3), 224-236.

Imbrici, P., Nematian-Ardestani, E., Hasan, S., Pessia, M., Tucker, S. J., & D’Adamo, M. C. (2020). Altered functional properties of a missense variant in the TRESK K+ channel (KCNK18) associated with migraine and intellectual disability. Pflügers Archiv-European Journal of Physiology, 472(7), 923-930.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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