Mouse Anti-KDM2A Recombinant Antibody (CBLXK-001) (CBMAB-2206CQ)

Name: Mouse Anti-KDM2A Recombinant Antibody (CBLXK-001)
SKU: CBMAB-2206CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLXK-001

Application

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-517

More Infomation

Target

Full Name

KDM2A

Introduction

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least six highly degenerated leucine-rich repeats. This family member plays a role in epigenetic silencing. It nucleates at CpG islands and specifically demethylates both mono- and di-methylated lysine-36 of histone H3. Alternative splicing results in multiple transcript variants. Among its related pathways are Chromatin organization and Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3. An important paralog of this gene is KDM2B.

Entrez Gene ID

22992

UniProt ID

Q9Y2K7

Alternative Names

KDM2A; lysine (K)-specific demethylase 2A; F box and leucine rich repeat protein 11, FBXL11; lysine-specific demethylase 2A; CXXC8; DKFZP434M1735; F box protein FBL11; FBL7; FBL11; FLJ00115; JHDM1A; jumonji C domain containing histone demethylase 1A; KIAA1004; LILINA; F-box/LRR-repeat protein 11; CXXC-type zinc finger protein 8; [Histone-H3]-lysine-36 demethylase 1A; F-box and leucine-rich repeat protein 11; jumonji C domain-containing histone demethylase 1A; jmjC domain-containing histone demethylation protein 1A; FBXL11; FLJ46431; DKFZp434M1735

Function

Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. Regulates circadian gene expression by repressing the transcriptional activator activity of CLOCK-ARNTL/BMAL1 heterodimer and RORA in a catalytically-independent manner (PubMed:26037310).

Biological Process

Chromatin organizationIEA:UniProtKB-KW
Circadian regulation of gene expressionManual Assertion Based On ExperimentIMP:UniProtKB
Double-strand break repair via nonhomologous end joiningManual Assertion Based On ExperimentIMP:UniProtKB
Histone H3-K36 demethylationManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of transcription by competitive promoter bindingManual Assertion Based On ExperimentIMP:UniProtKB
Protein demethylationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of circadian rhythmManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus, nucleoplasm. Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2.

Li, F., Wang, Y., Hwang, I., Jang, J. Y., Xu, L., Deng, Z., ... & Zheng, H. (2023). Histone demethylase KDM2A is a selective vulnerability of cancers relying on alternative telomere maintenance. Nature communications, 14(1), 1756.

Wang, J., Zhang, Z. Y., Jiang, J., Tang, L., Wang, X. Y., Wang, Z., ... & Ye, S. J. (2022). KDM2A plays a dual role in regulating the expression of malignancy-related genes in esophageal squamous cell carcinoma. Biochemical and Biophysical Research Communications, 624, 53-58.

Huang, X., Pan, J., Wang, G., Huang, T., Li, C., Wang, Y., & Li, X. (2021). UNC5B-AS1 promotes the proliferation, migration and EMT of hepatocellular carcinoma cells via regulating miR-4306/KDM2A axis. Cell Cycle, 20(20), 2114-2124.

Hua, Y., Yue, Y., Zhao, D., Ma, Y., Xiong, Y., Xiong, X., & Li, J. (2021). Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation. International Journal of Molecular Sciences, 22(18), 9759.

Liu, L., Liu, J., & Lin, Q. (2021). Histone demethylase KDM2A: Biological functions and clinical values. Experimental and Therapeutic Medicine, 22(1), 1-7.

Chen, L., Zhang, J., Zou, Y., Wang, F., Li, J., Sun, F., ... & Wang, C. Y. (2021). Kdm2a deficiency in macrophages enhances thermogenesis to protect mice against HFD-induced obesity by enhancing H3K36me2 at the Pparg locus. Cell Death & Differentiation, 28(6), 1880-1899.

Lin, Q., Wu, Z., Yue, X., Yu, X., Wang, Z., Song, X., ... & Ma, C. (2020). ZHX2 restricts hepatocellular carcinoma by suppressing stem cell-like traits through KDM2A-mediated H3K36 demethylation. EBioMedicine, 53.

Xu, W. H., Liang, D. Y., Wang, Q., Shen, J., Liu, Q. H., & Peng, Y. B. (2019). Knockdown of KDM2A inhibits proliferation associated with TGF-β expression in HEK293T cell. Molecular and Cellular Biochemistry, 456, 95-104.

Wang, F., Liang, S., Liu, X., Han, L., Wang, J., & Du, Q. (2018). LINC00460 modulates KDM2A to promote cell proliferation and migration by targeting miR-342-3p in gastric cancer. OncoTargets and therapy, 6383-6394.

Vacík, T., Lađinović, D., & Raška, I. (2018). KDM2A/B lysine demethylases and their alternative isoforms in development and disease. Nucleus, 9(1), 431-441.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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For research use only. Not intended for any clinical use.

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