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Rabbit Anti-KDM2A Recombinant Antibody (CBXF-1151) (CBMAB-F1019-CQ)

This product is a rabbit antibody that recognizes KDM2A. The antibody CBXF-1151 can be used for immunoassay techniques such as: WB, IP, IHC-P, IF.
See all KDM2A antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
CBXF-1151
Antibody Isotype
IgG
Application
WB, IP, IHC-P, IF

Basic Information

Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
KDM2A
Introduction
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least six highly degenerated leucine-rich repeats. This family member plays a role in epigenetic silencing. It nucleates at CpG islands and specifically demethylates both mono- and di-methylated lysine-36 of histone H3. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
Human22992
Mouse225876
Rat361700
UniProt ID
HumanQ9Y2K7
MouseP59997
RatD3ZTJ7
Alternative Names
Lysine Demethylase 2A; F-Box And Leucine-Rich Repeat Protein 11; JmjC Domain-Containing Histone Demethylation Protein 1A; Jumonji C Domain-Containing Histone Demethylase 1A; [Histone-H3]-Lysine-36 Demethylase 1A; Lysine (K)-Specific Demethylase 2A; CXXC-Type Zinc Finger Protein 8; F-Box/LRR-Repeat Protein 11; EC 1.14.11.27; FBXL11; JHDM1A;
Function
Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. Regulates circadian gene expression by repressing the transcriptional activator activity of CLOCK-ARNTL/BMAL1 heterodimer and RORA in a catalytically-independent manner (PubMed:26037310).
Biological Process
Chromatin organizationIEA:UniProtKB-KW
Circadian regulation of gene expressionManual Assertion Based On ExperimentIMP:UniProtKB
Double-strand break repair via nonhomologous end joiningManual Assertion Based On ExperimentIMP:UniProtKB
Histone H3-K36 demethylationManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of transcription by competitive promoter bindingManual Assertion Based On ExperimentIMP:UniProtKB
Protein demethylationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of circadian rhythmManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus, nucleoplasm. Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2.

Li, F., Wang, Y., Hwang, I., Jang, J. Y., Xu, L., Deng, Z., ... & Zheng, H. (2023). Histone demethylase KDM2A is a selective vulnerability of cancers relying on alternative telomere maintenance. Nature communications, 14(1), 1756.

Wang, J., Zhang, Z. Y., Jiang, J., Tang, L., Wang, X. Y., Wang, Z., ... & Ye, S. J. (2022). KDM2A plays a dual role in regulating the expression of malignancy-related genes in esophageal squamous cell carcinoma. Biochemical and Biophysical Research Communications, 624, 53-58.

Huang, X., Pan, J., Wang, G., Huang, T., Li, C., Wang, Y., & Li, X. (2021). UNC5B-AS1 promotes the proliferation, migration and EMT of hepatocellular carcinoma cells via regulating miR-4306/KDM2A axis. Cell Cycle, 20(20), 2114-2124.

Hua, Y., Yue, Y., Zhao, D., Ma, Y., Xiong, Y., Xiong, X., & Li, J. (2021). Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation. International Journal of Molecular Sciences, 22(18), 9759.

Liu, L., Liu, J., & Lin, Q. (2021). Histone demethylase KDM2A: Biological functions and clinical values. Experimental and Therapeutic Medicine, 22(1), 1-7.

Chen, L., Zhang, J., Zou, Y., Wang, F., Li, J., Sun, F., ... & Wang, C. Y. (2021). Kdm2a deficiency in macrophages enhances thermogenesis to protect mice against HFD-induced obesity by enhancing H3K36me2 at the Pparg locus. Cell Death & Differentiation, 28(6), 1880-1899.

Lin, Q., Wu, Z., Yue, X., Yu, X., Wang, Z., Song, X., ... & Ma, C. (2020). ZHX2 restricts hepatocellular carcinoma by suppressing stem cell-like traits through KDM2A-mediated H3K36 demethylation. EBioMedicine, 53.

Xu, W. H., Liang, D. Y., Wang, Q., Shen, J., Liu, Q. H., & Peng, Y. B. (2019). Knockdown of KDM2A inhibits proliferation associated with TGF-β expression in HEK293T cell. Molecular and Cellular Biochemistry, 456, 95-104.

Wang, F., Liang, S., Liu, X., Han, L., Wang, J., & Du, Q. (2018). LINC00460 modulates KDM2A to promote cell proliferation and migration by targeting miR-342-3p in gastric cancer. OncoTargets and therapy, 6383-6394.

Vacík, T., Lađinović, D., & Raška, I. (2018). KDM2A/B lysine demethylases and their alternative isoforms in development and disease. Nucleus, 9(1), 431-441.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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