Mouse Anti-KDM8 Antibody (V2-4829) (CBMAB-0577-YC)

Provided herein are mouse monoclonal antibodies against Human KDM8. The antibody clone 1A2 can be used for immunoassay techniques, such as IP, WB and MA.
See all KDM8 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-4829

Antibody Isotype

IgG2a

Application

IP, WB, MA

Basic Information

Immunogen

Recombinant protein

Specificity

Human

Antibody Isotype

IgG2a

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

KDM8

Introduction

KDM8 (lysine (K)-specific demethylase 8) is a histone lysine demethylase. KDM8 is a potential role for this protein as a tumor suppressor as studies in mouse shown.

Entrez Gene ID

79831

UniProt ID

Q8N371

Alternative Names

FLJ13798; JMJD5

Function

Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monooxygenase (PubMed:28847961, PubMed:29459673, PubMed:28982940, PubMed:29563586).
Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation (PubMed:28847961, PubMed:29459673, PubMed:28982940).
Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4. After initial cleavage, continues to digest histones tails via its aminopeptidase activity (PubMed:28847961, PubMed:29459673).
Upon DNA damage, cleaves the N-terminal tail of histone H3 at monomethylated lysine residues, preferably at monomethylated 'Lys-9' (H3K9me1). The histone variant H3F3A is the major target for cleavage (PubMed:28982940).
Additionnally, acts as Fe(2+) and 2-oxoglutarate-dependent monooxygenase, catalyzing (R)-stereospecific hydroxylation at C-3 of 'Arg-137' of RPS6 and 'Arg-141' of RCCD1, but the biological significance of this activity remains to be established (PubMed:29563586).
Regulates mitosis through different mechanisms: Plays a role in transcriptional repression of satellite repeats, possibly by regulating H3K36 methylation levels in centromeric regions together with RCCD1. Possibly together with RCCD1, is involved in proper mitotic spindle organization and chromosome segregation (PubMed:24981860).
Negatively regulates cell cycle repressor CDKN1A/p21, which controls G1/S phase transition (PubMed:24740926).
Required for G2/M phase cell cycle progression. Regulates expression of CCNA1/cyclin-A1, leading to cancer cell proliferation (PubMed:20457893).
Also, plays a role in regulating alpha-tubulin acetylation and cytoskeletal microtubule stability involved in epithelial to mesenchymal transition (PubMed:28455245).
Regulates the circadian gene expression in the liver (By similarity).
Represses the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer in a catalytically-independent manner (PubMed:30500822).
Negatively regulates the protein stability and function of CRY1; required for AMPK-FBXL3-induced CRY1 degradation (PubMed:30500822).

Biological Process

Chromatin organizationIEA:UniProtKB-KW
Circadian regulation of gene expressionISS:UniProtKB
G2/M transition of mitotic cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Histone H3-K36 demethylationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Protein destabilizationManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Nucleus; Chromosome. Colocalizes with trimethylated 'Lys-9' of histone H3 (H3K9me3).

References

Ahmed, A., Syed, J. N., Chi, L., Wang, Y., Perez-Romero, C., Lee, D., ... & Delgado-Olguín, P. (2023). KDM8 epigenetically controls cardiac metabolism to prevent initiation of dilated cardiomyopathy. Nature Cardiovascular Research, 2(2), 174-191.

Hsieh, C. C., Yang, C. Y., Peng, B., Ho, S. L., Tsao, C. H., Lin, C. K., ... & Chen, Y. W. (2023). Allyl Isothiocyanate Suppresses the Proliferation in Oral Squamous Cell Carcinoma via Mediating the KDM8/CCNA1 Axis. Biomedicines, 11(10), 2669.

Bakshi, S., & Wende, A. R. (2023). KDM8 prevents heart failure by controlling cardiac metabolism. Nature Cardiovascular Research, 2(2), 106-107.

Hsieh, C. C., Yang, C. Y., Tsao, C. H., Lin, C. K., Lin, C. S., Ho, S. L., ... & Chen, Y. W. (2023). Allyl isothiocyanate suppressed the proliferation of oral squamous cell carcinoma in vitro, in vivo, and in patient-derived tumor xenografts by downregulating the KDM8/CCNA1 axis.

Wang, H. J., Pochampalli, M., Wang, L. Y., Zou, J. X., Li, P. S., Hsu, S. C., ... & Kung, H. J. (2019). KDM8/JMJD5 as a dual coactivator of AR and PKM2 integrates AR/EZH2 network and tumor metabolism in CRPC. Oncogene, 38(1), 17-32.

Fuhrmann, D., Mernberger, M., Nist, A., Stiewe, T., & Elsässer, H. P. (2018). Miz1 controls Schwann cell proliferation via H3K36me2 demethylase Kdm8 to prevent peripheral nerve demyelination. Journal of Neuroscience, 38(4), 858-877.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-KDM8 Recombinant Antibody (1F3)

Mouse Anti-KDM8 Recombinant Antibody (CBFYK-002)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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