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Mouse Anti-KIF15 Recombinant Antibody (4D4) (CBMAB-K0845-LY)

This product is antibody recognizes KIF15. The antibody 4D4 immunoassay techniques such as: ELISA, WB.
See all KIF15 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4D4
Antibody Isotype
IgG2b, κ
Application
ELISA, WB

Basic Information

Immunogen
KIF15 (NP_064627, 1279 a.a. ~ 1386 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
kinesin family member 15
Introduction
Plus-end directed kinesin-like motor enzyme involved in mitotic spindle assembly.
Entrez Gene ID
56992
UniProt ID
Q9NS87
Alternative Names
Kinesin Family Member 15; Serologically Defined Breast Cancer Antigen NY-BR-62; Kinesin-Like Protein 2; Kinesin-Like Protein 7; Kinesin-Like 7; HKLP2;
Function
Plus-end directed kinesin-like motor enzyme involved in mitotic spindle assembly.
Biological Process
Microtubule-based movementManual Assertion Based On ExperimentIBA:GO_Central
Mitotic cell cycleManual Assertion Based On ExperimentTAS:ProtInc
Regulation of mitotic spindle assemblyIEA:InterPro
Cellular Location
Cytoplasm; Cytoplasm, cytoskeleton, spindle. Detected during the interphase in the cytoplasm as finely punctuate pattern and irregularly shaped dots. Detected during mitosis on the mitotic spindle. Colocalizes with TPX2 in mitosis. Localizes at the central spindle at anaphase (By similarity).
Localizes at the sites of invaginating cell membranes, a position that corresponds to the location of the contractile actomyosin ring of dividing cells (By similarity).
Colocalizes with actin in interphase (By similarity).
Colocalizes in dendrites and in growth cone of axons with microtubules (By similarity).

Cai, Y., Lai, Q., Zhang, X., Zhang, Y., Zhang, M., Gu, S., ... & Zhao, L. (2023). Kinesin superfamily member 15 knockdown inhibits cell proliferation, migration, and invasion in nasopharyngeal carcinoma. The Korean Journal of Physiology & Pharmacology: Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology, 27(5), 457-470.

Wang, L., Zhang, X., Liu, J., & Liu, Q. (2022). Kinesin family member 15 can promote the proliferation of glioblastoma. Mathematical Biosciences and Engineering, 19(8), 8259-8272.

Qureshi, Z., Ahmad, M., Yang, W. X., & Tan, F. Q. (2021). Kinesin 12 (KIF15) contributes to the development and tumorigenicity of prostate cancer. Biochemical and Biophysical Research Communications, 576, 7-14.

Li, Q., Qiu, J., Yang, H., Sun, G., Hu, Y., Zhu, D., ... & Jiang, R. (2020). Kinesin family member 15 promotes cancer stem cell phenotype and malignancy via reactive oxygen species imbalance in hepatocellular carcinoma. Cancer letters, 482, 112-125.

Wu, Z., Zhang, H., Sun, Z., Wang, C., Chen, Y., Luo, P., & Yan, W. (2020). Knockdown of kinesin family 15 inhibits osteosarcoma through suppressing cell proliferation and promoting cell apoptosis. Chemotherapy, 64(4), 187-196.

Malaby, H. L., Dumas, M. E., Ohi, R., & Stumpff, J. (2019). Kinesin-binding protein ensures accurate chromosome segregation by buffering KIF18A and KIF15. Journal of Cell Biology, 218(4), 1218-1234.

McHugh, T., Drechsler, H., McAinsh, A. D., Carter, N. J., & Cross, R. A. (2018). Kif15 functions as an active mechanical ratchet. Molecular Biology of the Cell, 29(14), 1743-1752.

Milic, B., Chakraborty, A., Han, K., Bassik, M. C., & Block, S. M. (2018). KIF15 nanomechanics and kinesin inhibitors, with implications for cancer chemotherapeutics. Proceedings of the National Academy of Sciences, 115(20), E4613-E4622.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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