Mouse Anti-KIF15 Recombinant Antibody (4D4) (CBMAB-K0845-LY)

Name: Mouse Anti-KIF15 Recombinant Antibody (4D4)
SKU: CBMAB-K0845-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4D4

Application

ELISA, WB

Immunogen

KIF15 (NP_064627, 1279 a.a. ~ 1386 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

kinesin family member 15

Introduction

Plus-end directed kinesin-like motor enzyme involved in mitotic spindle assembly.

Entrez Gene ID

56992

UniProt ID

Q9NS87

Alternative Names

Kinesin Family Member 15; Serologically Defined Breast Cancer Antigen NY-BR-62; Kinesin-Like Protein 2; Kinesin-Like Protein 7; Kinesin-Like 7; HKLP2;

Function

Plus-end directed kinesin-like motor enzyme involved in mitotic spindle assembly.

Biological Process

Microtubule-based movementManual Assertion Based On ExperimentIBA:GO_Central
Mitotic cell cycleManual Assertion Based On ExperimentTAS:ProtInc
Regulation of mitotic spindle assemblyIEA:InterPro

Cellular Location

Cytoplasm; Cytoplasm, cytoskeleton, spindle. Detected during the interphase in the cytoplasm as finely punctuate pattern and irregularly shaped dots. Detected during mitosis on the mitotic spindle. Colocalizes with TPX2 in mitosis. Localizes at the central spindle at anaphase (By similarity).
Localizes at the sites of invaginating cell membranes, a position that corresponds to the location of the contractile actomyosin ring of dividing cells (By similarity).
Colocalizes with actin in interphase (By similarity).
Colocalizes in dendrites and in growth cone of axons with microtubules (By similarity).

Cai, Y., Lai, Q., Zhang, X., Zhang, Y., Zhang, M., Gu, S., ... & Zhao, L. (2023). Kinesin superfamily member 15 knockdown inhibits cell proliferation, migration, and invasion in nasopharyngeal carcinoma. The Korean Journal of Physiology & Pharmacology: Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology, 27(5), 457-470.

Wang, L., Zhang, X., Liu, J., & Liu, Q. (2022). Kinesin family member 15 can promote the proliferation of glioblastoma. Mathematical Biosciences and Engineering, 19(8), 8259-8272.

Qureshi, Z., Ahmad, M., Yang, W. X., & Tan, F. Q. (2021). Kinesin 12 (KIF15) contributes to the development and tumorigenicity of prostate cancer. Biochemical and Biophysical Research Communications, 576, 7-14.

Li, Q., Qiu, J., Yang, H., Sun, G., Hu, Y., Zhu, D., ... & Jiang, R. (2020). Kinesin family member 15 promotes cancer stem cell phenotype and malignancy via reactive oxygen species imbalance in hepatocellular carcinoma. Cancer letters, 482, 112-125.

Wu, Z., Zhang, H., Sun, Z., Wang, C., Chen, Y., Luo, P., & Yan, W. (2020). Knockdown of kinesin family 15 inhibits osteosarcoma through suppressing cell proliferation and promoting cell apoptosis. Chemotherapy, 64(4), 187-196.

Malaby, H. L., Dumas, M. E., Ohi, R., & Stumpff, J. (2019). Kinesin-binding protein ensures accurate chromosome segregation by buffering KIF18A and KIF15. Journal of Cell Biology, 218(4), 1218-1234.

McHugh, T., Drechsler, H., McAinsh, A. D., Carter, N. J., & Cross, R. A. (2018). Kif15 functions as an active mechanical ratchet. Molecular Biology of the Cell, 29(14), 1743-1752.

Milic, B., Chakraborty, A., Han, K., Bassik, M. C., & Block, S. M. (2018). KIF15 nanomechanics and kinesin inhibitors, with implications for cancer chemotherapeutics. Proceedings of the National Academy of Sciences, 115(20), E4613-E4622.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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