Sign in or Register   Sign in or Register
  |  

Rabbit Anti-KIF20B Recombinant Antibody (EG1790) (CBMAB-EN2138-LY)

The product is antibody recognizes KIF20B. The antibody EG1790 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:40000.
See all KIF20B antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
EG1790
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 ELISA: 1:40000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from C-terminal of human KIF20B.
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Kinesin Family Member 20B
Introduction
Plus-end-directed motor enzyme that is required for completion of cytokinesis (PubMed:11470801, PubMed:12740395). Required for proper midbody organization and abscission in polarized cortical stem cells. Plays a role in the regulation of neuronal polarization by mediating the transport of specific cargos. Participates in the mobilization of SHTN1 and in the accumulation of PIP3 in the growth cone of primary hippocampal neurons in a tubulin and actin-dependent manner. In the developing telencephalon, cooperates with SHTN1 to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in cerebral cortex growth (By similarity). Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed:17409436).
Entrez Gene ID
9585
UniProt ID
Q96Q89
Alternative Names
Kinesin Family Member 20B; Cancer/Testis Antigen 90; M-Phase Phosphoprotein 1; Kinesin-Related Motor Interacting With PIN1; MPHOSPH1; KRMP1; CT90; MPP1;
Function
Plus-end-directed motor enzyme that is required for completion of cytokinesis (PubMed:11470801, PubMed:12740395).
Required for proper midbody organization and abscission in polarized cortical stem cells. Plays a role in the regulation of neuronal polarization by mediating the transport of specific cargos. Participates in the mobilization of SHTN1 and in the accumulation of PIP3 in the growth cone of primary hippocampal neurons in a tubulin and actin-dependent manner. In the developing telencephalon, cooperates with SHTN1 to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in cerebral cortex growth (By similarity).
Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed:17409436).
Biological Process
Cell cycleIEA:UniProtKB-KW
Cell divisionIEA:UniProtKB-KW
Microtubule-based movementManual Assertion Based On ExperimentIBA:GO_Central
Neural tube closureIEA:Ensembl
Neuron projection morphogenesisISS:UniProtKB
Positive regulation of cell population proliferationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of cytokinesisManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of intracellular protein transportISS:UniProtKB
Positive regulation of mitotic cytokinetic processISS:UniProtKB
Positive regulation of neuron migrationISS:UniProtKB
Protein localization to microtubuleISS:UniProtKB
Regulation of cell cycle1 PublicationNAS:UniProtKB
Regulation of establishment of cell polarityISS:UniProtKB
Regulation of mitotic nuclear division1 PublicationNAS:UniProtKB
Cellular Location
Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Nucleus, nucleolus; Nucleus, nucleoplasm; Cytoplasm, cytoskeleton, spindle; Cytoplasm, cytoskeleton, spindle pole; Midbody; Cell projection, axon; Cell projection, growth cone. Localizes mainly in the nucleus during interphase although it is also detected in the cytoplasm without clear association with microtubules (PubMed:12740395).
Localized to the central spindle during cytokinetic furrowing and with the midbody during abscission (PubMed:12740395, PubMed:17409436).
A 2-3 fold expression increase is seen as cells progress from G1 to G2/M phase (PubMed:12740395).
During prophase and metaphase it is found throughout the cytoplasm and at anaphase accumulates at the midplan of the cell and forms a distinct band extending across the spindle midzone (PubMed:12740395).
At anaphase it is concentrated in the midbody (PubMed:12740395).
Colocalized partially along microtubules in primary neurons. Colocalized with SHTN1 along microtubules to the tip of the growing cone in primary hippocampal neurons. Localized in midbodies between dividing radial progenitors in the ventricular zone (By similarity).
Colocalized with PRC1 in the nucleus of bladder carcinoma cells at the interphase. Colocalized with PRC1 in bladder carcinoma cells at prophase, metaphase, early anaphase, at the midzone in late anaphase and at the contractile ring in telophase (PubMed:17409436).
PTM
Phosphorylated during mitosis by CDK1 (PubMed:11470801, PubMed:12740395).

Koizumi, H., Muro, Y., Yamashita, Y., Takeichi, T., Fritzler, M. J., & Akiyama, M. (2023). Anti‐KIF20B autoantibodies in systemic autoimmune rheumatic diseases: Their high prevalence in systemic lupus erythematosus. The Journal of Dermatology.

Chen, J., Zhao, C. C., Chen, F. R., Feng, G. W., Luo, F., & Jiang, T. (2021). KIF20B promotes cell proliferation and may be a potential therapeutic target in pancreatic cancer. Journal of Oncology, 2021.

Fritzler, M. J., Brown, R. D., & Zhang, M. (2019). A monoclonal antibody to M-phase phosphoprotein 1/Kinesin-like protein KIF20B. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 38(4), 162-170.

Georges, A., Coyaud, E., Marcon, E., Greenblatt, J., Raught, B., & Frappier, L. (2019). USP7 regulates cytokinesis through FBXO38 and KIF20B. Scientific Reports, 9(1), 2724.

Li, G., Xie, Z. K., Zhu, D. S., Guo, T., Cai, Q. L., & Wang, Y. (2019). KIF20B promotes the progression of clear cell renal cell carcinoma by stimulating cell proliferation. Journal of Cellular Physiology, 234(9), 16517-16525.

Lin, W. F., Lin, X. L., Fu, S. W., Yang, L., Tang, C. T., Gao, Y. J., ... & Ge, Z. Z. (2018). Pseudopod‐associated protein KIF20B promotes Gli1‐induced epithelial‐mesenchymal transition modulated by pseudopodial actin dynamic in human colorectal cancer. Molecular Carcinogenesis, 57(7), 911-925.

Janisch, K. M., McNeely, K. C., Dardick, J. M., Lim, S. H., & Dwyer, N. D. (2018). Kinesin-6 KIF20B is required for efficient cytokinetic furrowing and timely abscission in human cells. Molecular Biology of the Cell, 29(2), 166-179.

Little, J. N., & Dwyer, N. D. (2018). p53 deletion rescues apoptosis and microcephaly in a Kif20b mouse mutant. bioRxiv, 272393.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rabbit Anti-KIF20B Recombinant Antibody (EG1790)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare