Mouse Anti-KIF9 Recombinant Antibody (4E9) (CBMAB-K0898-LY)

Name: Mouse Anti-KIF9 Recombinant Antibody (4E9)
SKU: CBMAB-K0898-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4E9

Application

ELISA, WB

Immunogen

KIF9 (NP_878905.1, 691 a.a. ~ 789 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: VDQCRHRLLM EFDIWYNESF VIPEDMQMAL KPGGSIRPGM VPVNRIVSLG EDDQDKFSQL QQRVLPEGPD SISFYNAKVK IEQKHNYLKT MMGLQQAHR

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

kinesin family member 9

Introduction

KIF9 (Kinesin Family Member 9) is a Protein Coding gene. Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Golgi-to-ER retrograde transport. Gene Ontology (GO) annotations related to this gene include ATPase activity and microtubule binding. An important paralog of this gene is KIF6.

Entrez Gene ID

64147

UniProt ID

Q9HAQ2

Alternative Names

Kinesin Family Member 9;

Function

Essential for normal male fertility and for progressive motility of spermatozoa.

Biological Process

Extracellular matrix disassemblyManual Assertion Based On ExperimentIMP:UniProtKB
Microtubule-based movementManual Assertion Based On ExperimentIBA:GO_Central
Organelle disassemblyManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of flagellated sperm motilityISS:UniProtKB
Regulation of podosome assemblyManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cytoplasm, cytoskeleton; Cytoplasm, cytoskeleton, flagellum axoneme

Konjikusic, M. J., Lee, C., Yue, Y., Shrestha, B. D., Nguimtsop, A. M., Horani, A., ... & Wallingford, J. B. (2023). Kif9 is an active kinesin motor required for ciliary beating and proximodistal patterning of motile axonemes. Journal of cell science, 136(5), jcs259535.

Meng, Z., Meng, Q., Gao, T., Zhou, H., Xue, J., Li, H., ... & Lv, J. (2023). Identification of bi-allelic KIF9 loss-of-function variants contributing to asthenospermia and male infertility in two Chinese families. Frontiers in Endocrinology, 13, 1091107.

Yu, Y., Lu, X., Yan, Y., Wang, Y., Meng, J., Tian, S., & Mu, J. (2022). The lncRNA KIF9-AS1 accelerates hepatocellular carcinoma growth by recruiting DNMT1 to promote RAI2 DNA methylation. Journal of Oncology, 2022.

Miyata, H., Oyama, Y., Kaneda, Y., & Ikawa, M. (2022). The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum. Experimental Animals, 71(1), 46-52.

Yao, J., Gao, R., Luo, M., Li, D., Guo, L., Yu, Z., ... & Wang, L. (2021). Long noncoding RNA KIF9-AS1 promotes cell apoptosis by targeting the microRNA-148a-3p/suppressor of cytokine signaling axis in inflammatory bowel disease. European Journal of Gastroenterology & Hepatology, 33(Suppl 1), e922.

Miyata, H., Shimada, K., Morohoshi, A., Oura, S., Matsumura, T., Xu, Z., ... & Ikawa, M. (2020). Testis‐enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa. The FASEB Journal, 34(4), 5389-5400.

Jin, Y., Huang, R., Xia, Y., Huang, C., Qiu, F., Pu, J., ... & Zhao, X. (2020). Long noncoding RNA KIF9-AS1 regulates transforming growth factor-β and autophagy signaling to enhance renal cell carcinoma chemoresistance via microRNA-497-5p. DNA and cell biology, 39(7), 1096-1103.

You, H., Wang, S., & Yu, S. (2020). KIF9‑AS1 promotes nasopharyngeal carcinoma progression by suppressing miR‑16. Oncology Letters, 20(5), 1-1.

Wang, S., Hou, Y., Chen, W., Wang, J., Xie, W., Zhang, X., & Zeng, L. (2018). KIF9‑AS1, LINC01272 and DIO3OS lncRNAs as novel biomarkers for inflammatory bowel disease. Molecular medicine reports, 17(2), 2195-2202.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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