Mouse Anti-KIRREL1 Recombinant Antibody (G-8) (CBMAB-N0886-WJ)

This product is a Mouse antibody that recognizes KIRREL1. The antibody G-8 can be used for immunoassay techniques such as: WB, IP, IF, ELISA.
See all KIRREL1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Mouse, Rat, Human

Clone

G-8

Application

WB, IP, IF, ELISA

Basic Information

Specificity

Mouse, Rat, Human

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Kirre Like Nephrin Family Adhesion Molecule 1

Introduction

NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).[supplied by OMIM, Mar 2008]

Entrez Gene ID

Human	55243
Rat	310695

UniProt ID

Human	Q96J84
Mouse	Q80W68
Rat	Q6X936

Alternative Names

Kirre Like Nephrin Family Adhesion Molecule 1; Nephrin-Like Protein 1; Kin Of Irregular Chiasm-Like Protein 1; KIRREL; NEPH1; Kin Of IRRE Like (Drosophila); Kin Of IRRE-Like Protein 1; Kin Of IRRE Like; Nephrin Related;

Function

Required for proper function of the glomerular filtration barrier. It is involved in the maintenance of a stable podocyte architecture with interdigitating foot processes connected by specialized cell-cell junctions, known as the slit diaphragm (PubMed:31472902).
It is a signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1 (By similarity).

Biological Process

Cell-cell adhesionManual Assertion Based On ExperimentIBA:GO_Central
Cell-cell junction maintenanceManual Assertion Based On ExperimentIMP:UniProtKB
ExcretionIEA:Ensembl
Glomerular filtrationManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of protein phosphorylationIEA:Ensembl
Positive regulation of actin filament polymerizationIEA:Ensembl

Cellular Location

Cell membrane. Predominantly located at podocyte slit diaphragm.

Involvement in disease

Nephrotic syndrome 23 (NPHS23):
A form of nephrotic syndrome, a renal disease clinically characterized by severe proteinuria, resulting in complications such as hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form that progresses to end-stage renal failure. NPHS23 is an autosomal recessive form characterized by onset of proteinuria in the first or second decade of life, and variable outcome. Some patients have normal renal function after many years, whereas others may progress to chronic kidney disease.

Topology

Extracellular: 17-499
Helical: 500-520
Cytoplasmic: 521-757

PTM

Phosphorylation probably regulates the interaction with NSH2. Phosphorylated at Tyr-605 and Tyr-606 by FYN, leading to GRB2 binding (By similarity).
N-glycosylated.

References

Paul, A., Annunziato, S., Lu, B., Sun, T., Evrova, O., Planas-Paz, L., ... & Cong, F. (2022). Cell adhesion molecule KIRREL1 is a feedback regulator of Hippo signaling recruiting SAV1 to cell-cell contact sites. Nature Communications, 13(1), 930.

Gu, Y., Wang, Y., Sha, Z., He, C., Zhu, Y., Li, J., ... & Yu, F. X. (2022). Transmembrane protein KIRREL1 regulates Hippo signaling via a feedback loop and represents a therapeutic target in YAP/TAZ-active cancers. Cell Reports, 40(9).

Solanki, A. K., Widmeier, E., Arif, E., Sharma, S., Daga, A., Srivastava, P., ... & Nihalani, D. (2019). Mutations in KIRREL1, a slit diaphragm component, cause steroid-resistant nephrotic syndrome. Kidney international, 96(4), 883-889.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-KIRREL1 Recombinant Antibody (G-8)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote

Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: