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Mouse Anti-KLF8 Recombinant Antibody (2E10) (CBMAB-K1128-LY)

This product is antibody recognizes KLF8. The antibody 2E10 immunoassay techniques such as: ELISA, WB.
See all KLF8 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2E10
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
KLF8 (NP_009181.1, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: MVDMDKLINN LEVQLNSEGG SMQVFKQVTA SVRNRDPPEI EYRSNMTSPT LLDANPMENP ALFNDIKIEP PEELLASDFS LPQVEPVDLS FHKPKAPL
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Kruppel-like factor 8
Introduction
This gene encodes a protein which is a member of the Sp/KLF family of transcription factors. Members of this family contain a C-terminal DNA-binding domain with three Kruppel-like zinc fingers. The encoded protein is thought to play an important role in the regulation of epithelial to mesenchymal transition, a process which occurs normally during development but also during metastasis. A pseudogene has been identified on chromosome 16. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
Entrez Gene ID
11279
UniProt ID
O95600
Alternative Names
Kruppel Like Factor 8; Basic Krueppel-Like Factor 3; Zinc Finger Protein 741; ZNF741; BKLF3; Basic Kruppel-Like Factor 3; Krueppel-Like Factor 8;
Function
Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK).
Biological Process
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:NTNU_SB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
PTM
Sumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G(1) phase. Has no effect on subcellular location.

Le Minh, G., Esquea, E. M., Dhameliya, T. T., Merzy, J., Lee, M. H., Ball, L. E., & Reginato, M. J. (2023). Kruppel-like factor 8 regulates triple negative breast cancer stem cell-like activity. Frontiers in Oncology, 13, 1141834.

Cherukunnath, A., Davargaon, R. S., Ashraf, R., Kamdar, U., Srivastava, A. K., Tripathi, P. P., ... & Kumar, S. (2022). KLF8 is activated by TGF‐β1 via Smad2 and contributes to ovarian cancer progression. Journal of Cellular Biochemistry, 123(5), 921-934.

Kumar, S., Behera, A., Saha, P., & Srivastava, A. K. (2021). The role of Krüppel-like factor 8 in cancer biology: Current research and its clinical relevance. Biochemical Pharmacology, 183, 114351.

Wang, M. D., Xing, H., Li, C., Liang, L., Wu, H., Xu, X. F., ... & Yang, T. (2020). A novel role of Krüppel-like factor 8 as an apoptosis repressor in hepatocellular carcinoma. Cancer Cell International, 20(1), 1-12.

Zhao, M., Ma, W., & Ma, C. (2020). Circ_0067934 promotes non-small cell lung cancer development by regulating miR-1182/KLF8 axis and activating Wnt/β-catenin pathway. Biomedicine & Pharmacotherapy, 129, 110461.

Mao, A., Zhou, X., Liu, Y., Ding, J., Miao, A., & Pan, G. (2019). KLF8 is associated with poor prognosis and regulates glycolysis by targeting GLUT4 in gastric cancer. Journal of cellular and molecular medicine, 23(8), 5087-5097.

Liu, J., Zheng, H., Ding, Y., Li, M., Li, J., Guo, J., ... & Xiong, S. (2019). The level of Krüppel-like factor 8 expression predicts prognosis and metastasis in various carcinomas. Medicine, 98(18).

Ma, D., Liu, H., Qin, Y., Tian, Z., Li, S., & Liang, N. (2019). KLF8 overexpression promotes the growth of human lung cancer cells by promoting the expression of JMJD2A. Cancer Cell International, 19, 1-10.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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