Mouse Anti-KLHL1 Recombinant Antibody (CBT4706) (V2LY-0625-LY2523)

Name: Mouse Anti-KLHL1 Recombinant Antibody (CBT4706)
SKU: V2LY-0625-LY2523
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBT4706

Antibody Isotype

IgG1

Application

ICC

Basic Information

Immunogen

Purified recombinant fragment of human KLHL1 expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:200-1:1,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Preservative

Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Kelch Like Family Member 1

Entrez Gene ID

57626

UniProt ID

Q9NR64

Function

May play a role in organizing the actin cytoskeleton of the brain cells.

Biological Process

Actin cytoskeleton organization1 PublicationNAS:UniProtKB
Adult walking behaviorIEA:Ensembl
Cerebellar Purkinje cell layer developmentIEA:Ensembl
Dendrite developmentManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm, cytoskeleton

More Infomation

References

Perissinotti, P. P., Martínez-Hernández, E., He, Y., Koob, M. D., & Piedras-Rentería, E. S. (2021). Genetic deletion of KLHL1 leads to hyperexcitability in hypothalamic POMC neurons and lack of electrical responses to leptin. Frontiers in Neuroscience, 15, 718464.

Martínez-Hernández, E., Zeglin, A., Almazan, E., Perissinotti, P., He, Y., Koob, M., ... & Piedras-Rentería, E. S. (2020). KLHL1 controls CaV3. 2 expression in DRG neurons and mechanical sensitivity to pain. Frontiers in Molecular Neuroscience, 12, 315.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote
Second antibody and isotype control

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