Mouse Anti-KLHL20 Recombinant Antibody (CBLY1-117) (CBMAB-K1190-LY)

This product is antibody recognizes KLHL20. The antibody CBLY1-117 immunoassay techniques such as: WB, IP, IF, ELISA.
See all KLHL20 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBLY1-117

Antibody Isotype

IgG

Application

WB, IP, IF, ELISA

Basic Information

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Kelch Like Family Member 20

Introduction

The protein encoded by this gene is a member of the kelch family of proteins, which is characterized by a 44-56 amino acid repeat motif. The kelch motif appears in many different polypeptide contexts and contains multiple potential protein-protein contact sites. Members of this family are present both throughout the cell and extracellularly, with diverse activities. [provided by RefSeq, Jul 2008]

Entrez Gene ID

27252

UniProt ID

Q9Y2M5

Alternative Names

Kelch Like Family Member 20; Kelch-Like ECT2-Interacting Protein; Kelch-Like Protein X; KLEIP; KLHLX; Kelch Motif Containing Protein; Kelch-Like 20 (Drosophila); Kelch-Like Protein 20; KHLHX;

Function

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response and anterograde Golgi to endosome transport. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis (PubMed:20389280).
The BCR(KLHL20) E3 ubiquitin ligase complex also specifically mediates 'Lys-33'-linked ubiquitination (PubMed:24768539).
Involved in anterograde Golgi to endosome transport by mediating 'Lys-33'-linked ubiquitination of CORO7, promoting interaction between CORO7 and EPS15, thereby facilitating actin polymerization and post-Golgi trafficking (PubMed:24768539).
Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases. The BCR(KLHL20) E3 ubiquitin ligase complex acts as a regulator of neurite outgrowth by mediating ubiquitination and degradation of PDZ-RhoGEF/ARHGEF11 (PubMed:21670212).
In case of tumor, the BCR(KLHL20) E3 ubiquitin ligase complex is involved in tumor hypoxia: following hypoxia, the BCR(KLHL20)complex mediates ubiquitination and degradation of PML, potentiating HIF-1 signaling and cancer progression (PubMed:21840486).

Biological Process

Cytoskeleton organizationManual Assertion Based On ExperimentTAS:ProtInc
Golgi to endosome transportManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Protein K33-linked ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Protein transportIEA:UniProtKB-KW
Protein ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Response to interferon-alphaManual Assertion Based On ExperimentTAS:UniProtKB

Cellular Location

Cytoplasm, perinuclear region; Nucleus; Golgi apparatus, trans-Golgi network; Cell projection, axon; Cell projection, dendrite. Localizes in the perinuclear region in normal conditions. Following IFN-alpha or IFN-gamma treatment, it is relocalized and sequestrated to the PML nuclear bodies, preventing DAPK1 ubiquitination (PubMed:20389280).

References

Choi, S. H., Cho, S. Y., Park, S. Y., & Hur, M. W. (2023). Post-translational regulation of proto-oncogene ZBTB7A expression by p53 status in cancer cells: HSP90-dependent stabilization vs. p53-KLHL20-ubiquitin proteasomal degradation. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1866(2), 194931.

Farrell, B. M., Gerth, F., Yang, C. H. R., & Yeh, J. T. H. (2022). A synthetic KLHL20 ligand to validate CUL3KLHL20 as a potent E3 ligase for targeted protein degradation. Genes & Development, 36(17-18), 1031-1042.

Sleyp, Y., Valenzuela, I., Accogli, A., Ballon, K., Ben-Zeev, B., Berkovic, S. F., ... & University of Washington Center for Mendelian Genomics. (2022). De novo missense variants in the E3 ubiquitin ligase adaptor KLHL20 cause a developmental disorder with intellectual disability, epilepsy, and autism spectrum disorder. Genetics in Medicine, 24(12), 2464-2474.

Li, S., Li, R., Ahmad, I., Liu, X., Johnson, S. F., Sun, L., & Zheng, Y. H. (2022). Cul3-KLHL20 E3 ubiquitin ligase plays a key role in the arms race between HIV-1 Nef and host SERINC5 restriction. Nature Communications, 13(1), 2242.

Li, R., Ahmad, I., Li, S., Johnson, S., Sun, L., & Zheng, Y. H. (2021). Cul3-KLHL20 E3 ubiquitin ligase plays a key role in the arms race between HIV-1 and host restriction.

Chen, Z., Picaud, S., Filippakopoulos, P., D'Angiolella, V., & Bullock, A. N. (2019). Structural basis for recruitment of DAPK1 to the KLHL20 E3 ligase. Structure, 27(9), 1395-1404.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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