Rat Anti-KLRK1 Recombinant Antibody (14L196) (CBMAB-C3115-LY)

Name: Rat Anti-KLRK1 Recombinant Antibody (14L196)
SKU: CBMAB-C3115-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

14L196

Application

Immunogen

Recombinant protein corresponding to mouse NKG2D

Specificity

Mouse

Antibody Isotype

IgG2

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Concentration

0.5 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Killer Cell Lectin Like Receptor K1

Introduction

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. This gene encodes a member of the NKG2 family. The encoded transmembrane protein is characterized by a type II membrane orientation (has an extracellular C terminus) and the presence of a C-type lectin domain. It binds to a diverse family of ligands that include MHC class I chain-related A and B proteins and UL-16 binding proteins, where ligand-receptor interactions can result in the activation of NK and T cells. The surface expression of these ligands is important for the recognition of stressed cells by the immune system, and thus this protein and its ligands are therapeutic targets for the treatment of immune diseases and cancers. Read-through transcription exists between this gene and the upstream KLRC4 (killer cell lectin-like receptor subfamily C, member 4) family member in the same cluster. [provided by RefSeq, Dec 2010]

Entrez Gene ID

27007

UniProt ID

O54709

Function

Functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, RAET1L/ULBP6, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.

Biological Process

Adaptive immune responseIEA:UniProtKB-KW
Cell differentiationIEA:UniProtKB-KW
Cellular response to lipopolysaccharideIEA:Ensembl
Defense response to Gram-positive bacteriumIEA:Ensembl
Natural killer cell activationManual Assertion Based On ExperimentTAS:BHF-UCL
Natural killer cell mediated cytotoxicityManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of GTPase activityManual Assertion Based On ExperimentIDA:CACAO
Negative regulation of natural killer cell chemotaxisManual Assertion Based On ExperimentIDA:CACAO
Positive regulation of interferon-gamma productionIEA:Ensembl
Positive regulation of natural killer cell mediated cytotoxicityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of nitric oxide biosynthetic processIEA:Ensembl
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Stimulatory C-type lectin receptor signaling pathwayIEA:Ensembl
T cell costimulationManual Assertion Based On ExperimentTAS:BHF-UCL

Cellular Location

Cell membrane
Colocalized with HCST on the cell surface.

Topology

Cytoplasmic: 1-51
Helical: 52-72
Extracellular: 73-216

Tan, H., Yang, H., Qian, J., Liu, S., Yan, D., Wei, L., & Liu, W. (2023). Involvement of KLRK1 in immune infiltration of head and neck squamous cell carcinoma correlates with favorable prognosis. Medicine, 102(32), e34761.

Weiss, E., Schrüfer, A., Tocantins, C., Diniz, M. S., Novakovic, B., van Bergen, A. S., ... & Hiden, U. (2023). Higher gestational weight gain delays wound healing and reduces expression of long non‐coding RNA KLRK1‐AS1 in neonatal endothelial progenitor cells. The Journal of Physiology, 601(17), 3961-3974.

Tsyklauri, O., Chadimova, T., Niederlova, V., Kovarova, J., Michalik, J., Malatova, I., ... & Stepanek, O. (2023). Regulatory T cells suppress the formation of potent KLRK1 and IL-7R expressing effector CD8 T cells by limiting IL-2. Elife, 12, e79342.

Zhang, Y., Chen, Z., Jiang, A., & Gao, G. (2022). KLRK1 as a prognostic biomarker for lung adenocarcinoma cancer. Scientific Reports, 12(1), 1976.

Wang, J., Dai, Z., Miao, Y., Zhao, T., Gan, J., Zhao, C., ... & Guan, Q. (2021). Carbon ion (12C6+) irradiation induces the expression of Klrk1 in lung cancer and optimizes the tumor microenvironment based on the NKG2D/NKG2D-Ls pathway. Cancer Letters, 521, 178-195.

von Linsingen, R., de França, P. P., de Carvalho, N. S., & da Graça Bicalho, M. (2020). MICA and KLRK1 genes and their impact in cervical intraepithelial neoplasia development in the southern Brazilian population. Human Immunology, 81(5), 249-253.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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