Rabbit Anti-KMT2B Recombinant Antibody (D6X2E) (CBMAB-CP3077-LY)

Name: Rabbit Anti-KMT2B Recombinant Antibody (D6X2E)
SKU: CBMAB-CP3077-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D6X2E

Application

WB, IP, IF (ICC)

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MLL2/KMT2B protein. This antibody detects the full-length 600 kDa MLL2/KMT2B protein and the Taspase 1-cleaved 80 kDa C-terminal MLL2/KMT2B protein (MLL2/KMT2B-C).

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Lysine Methyltransferase 2B

Introduction

This gene encodes a protein which contains multiple domains including a CXXC zinc finger, three PHD zinc fingers, two FY-rich domains, and a SET (suppressor of variegation, enhancer of zeste, and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This gene is ubiquitously expressed in adult tissues. It is also amplified in solid tumor cell lines, and may be involved in human cancer. Two alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene, however, the full length nature of the shorter transcript is not known. [provided by RefSeq, Jul 2008]

Entrez Gene ID

9757

UniProt ID

Q9UMN6

Alternative Names

Lysine Methyltransferase 2B; Myeloid/Lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog; Drosophila) 4; Myeloid/Lymphoid Or Mixed-Lineage Leukemia Protein 4; Lysine (K)-Specific Methyltransferase 2B; WBP-7; HRX2; MLL2; MLL4; TRX2; WBP7; Histone-Lysine N-Methyltransferase MLL4; Histone-Lysine N-Methyltransferase 2B;

Function

Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery predominantly forms H3K4me1 and H3K4me2 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed:25561738, PubMed:17707229).
Likely plays a redundant role with KMT2C in enriching H3K4me1 marks on primed and active enhancer elements (PubMed:24081332).
Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2 (PubMed:17707229).
Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development (By similarity).
Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation (By similarity).

Biological Process

Chromatin organizationIEA:UniProtKB-KW
Histone H3-K4 dimethylationManual Assertion Based On ExperimentIDA:CACAO
Histone H3-K4 methylationManual Assertion Based On ExperimentIMP:UniProtKB
Histone H3-K4 monomethylationManual Assertion Based On ExperimentIDA:CACAO
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus

Involvement in disease

Dystonia 28, childhood-onset (DYT28):
A form of dystonia, a disorder defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT28 is an autosomal dominant, progressive form characterized by onset in the first decade of life and variable severity. Dystonia begins focally in the lower limbs, resulting in gait difficulties, with later progression to other body regions, including the upper limbs, neck, and orofacial region.

Zhao, D., Yuan, H., Fang, Y., Gao, J., Li, H., Li, M., ... & Gan, Y. (2023). Histone Methyltransferase KMT2B Promotes Metastasis and Angiogenesis of Cervical Cancer by Upregulating EGF Expression. International Journal of Biological Sciences, 19(1), 34.

Feng, J. F., Wang, J., Xie, G., Wang, Y. D., Li, X. H., Yang, W. Y., ... & Zhang, B. (2022). KMT2B promotes the growth of renal cell carcinoma via upregulation of SNHG12 expression and promotion of CEP55 transcription. Cancer Cell International, 22(1), 197.

Lee, S., Ochoa, E., Barwick, K., Cif, L., Rodger, F., Docquier, F., ... & Maher, E. R. (2022). Comparison of methylation episignatures in KMT2B-and KMT2D-related human disorders. Epigenomics, 14(9), 537-547.

Klonou, A., Chlamydas, S., & Piperi, C. (2021). Structure, activity and function of the MLL2 (KMT2B) protein lysine methyltransferase. Life, 11(8), 823.

Leng, X., Wang, J., An, N., Wang, X., Sun, Y., & Chen, Z. (2020). Histone 3 lysine-27 demethylase KDM6A coordinates with KMT2B to play an oncogenic role in NSCLC by regulating H3K4me3. Oncogene, 39(41), 6468-6479.

Cif, L., Demailly, D., Lin, J. P., Barwick, K. E., Sa, M., Abela, L., ... & Kurian, M. A. (2020). KMT2B-related disorders: expansion of the phenotypic spectrum and long-term efficacy of deep brain stimulation. Brain, 143(11), 3242-3261.

Zech, M., Lam, D. D., & Winkelmann, J. (2019). Update on KMT2B-related dystonia. Current neurology and neuroscience reports, 19, 1-11.

Kawarai, T., Miyamoto, R., Nakagawa, E., Koichihara, R., Sakamoto, T., Mure, H., ... & Kaji, R. (2018). Phenotype variability and allelic heterogeneity in KMT2B-Associated disease. Parkinsonism & Related Disorders, 52, 55-61.

Gorman, K. M., Meyer, E., & Kurian, M. A. (2018). Review of the phenotype of early-onset generalised progressive dystonia due to mutations in KMT2B. European Journal of Paediatric Neurology, 22(2), 245-256.

Tomizawa, S. I., Kobayashi, Y., Shirakawa, T., Watanabe, K., Mizoguchi, K., Hoshi, I., ... & Ohbo, K. (2018). Kmt2b conveys monovalent and bivalent H3K4me3 in mouse spermatogonial stem cells at germline and embryonic promoters. Development, 145(23), dev169102.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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