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Rabbit Anti-KMT5A Recombinant Antibody (CBXS-5992) (CBMAB-S0838-CQ)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey, Cattle, Pig, Horse
Clone
CBXS-5992
Antibody Isotype
IgG
Application
WB, IF

Basic Information

Specificity
Human, Mouse, Rat, Monkey, Cattle, Pig, Horse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Lysine Methyltransferase 5A
Introduction
The protein encoded by this gene is a protein-lysine N-methyltransferase that can monomethylate Lys-20 of histone H4 to effect transcriptional repression of some genes. The encoded protein is required for cell proliferation and plays a role in chromatin condensation.
Entrez Gene ID
Human387893
Mouse67956
Rat689820
Monkey709345
Cattle532622
Pig100626555
Horse100060853
UniProt ID
HumanQ9NQR1
MouseQ2YDW7
RatD3ZEB9
MonkeyA0A1D5RB82
CattleQ2YDJ8
PigA0A5G2QGS9
HorseF6VW80
Alternative Names
Lysine Methyltransferase 5A; SET Domain Containing (Lysine Methyltransferase) 8; Lysine (K)-Specific Methyltransferase 5A; Histone-Lysine N-Methyltransferase KMT5A; PR/SET Domain-Containing Protein 07; SET Domain-Containing Protein 8; Lysine N-Methyltransferase 5A; Lysine-Specific Methylase 5A; H4-K20-HMTase KMT5A; EC 2.1.1.43; PR/SET07; PR-Set7; SETD8;
Function
Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration.
Biological Process
Cell divisionIEA:UniProtKB-KW
Chromatin organizationIEA:UniProtKB-KW
Mitotic chromosome condensationTAS:Reactome
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Peptidyl-lysine monomethylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of DNA damage response, signal transduction by p53 class mediatorManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of signal transduction by p53 class mediatorTAS:Reactome
Cellular Location
Nucleus
Chromosome
Specifically localizes to mitotic chromosomes. Colocalized with SIRT2 at mitotic foci. Associates with chromosomes during mitosis; association is increased in a H(2)O(2)-induced oxidative stress-dependent manner. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
PTM
Acetylated at Lys-172; does not change methyltransferase activity. Deacetylated at Lys-172 by SIRT2; does not change methyltransferase activity.
Ubiquitinated and degraded by the DCX(DTL) complex.
More Infomation

Crain, A. T., Klusza, S., Armstrong, R. L., Santa Rosa, P., Temple, B. R., Strahl, B. D., ... & Duronio, R. J. (2022). Distinct developmental phenotypes result from mutation of Set8/KMT5A and histone H4 lysine 20 in Drosophila melanogaster. Genetics, 221(2), iyac054.

Yu, B., Su, J., Shi, Q., Liu, Q., Ma, J., Ru, G., ... & Tang, J. (2022). KMT5A-methylated SNIP1 promotes triple-negative breast cancer metastasis by activating YAP signaling. Nature Communications, 13(1), 2192.

An, J., Zhang, J., Wang, Z., Wang, K., Liang, W., Liu, X., ... & Wang, S. (2022). KMT5A Knockdown Suppresses Osteosarcoma Cell Proliferation and Metastasis Through Ꞵ-Catenin Signalling. Clinical and Investigative Medicine, 45(3), E23-31.

Lu, X., Xu, M., Zhu, Q., Zhang, J., Liu, G., Bao, Y., ... & Zhu, W. G. (2021). RNF8‐ubiquitinated KMT5A is required for RNF168‐induced H2A ubiquitination in response to DNA damage. The FASEB Journal, 35(4), e21326.

Li, J., Qian, Y., Zhang, C., Wang, W., Qiao, Y., Song, H., ... & Deng, X. (2021). LncRNA LINC00473 is involved in the progression of invasive pituitary adenoma by upregulating KMT5A via ceRNA-mediated miR-502-3p evasion. Cell Death & Disease, 12(6), 580.

Lu, L., Li, X., Zhong, Z., Zhou, W., Zhou, D., Zhu, M., & Miao, C. (2021). KMT5A downregulation participated in High Glucose-mediated EndMT via Upregulation of ENO1 Expression in Diabetic Nephropathy. International Journal of Biological Sciences, 17(15), 4093.

Huang, T., Li, X., Wang, F., Lu, L., Hou, W., Zhu, M., & Miao, C. (2021). The CREB/KMT5A complex regulates PTP1B to modulate high glucose-induced endothelial inflammatory factor levels in diabetic nephropathy. Cell Death & Disease, 12(4), 333.

Lu, L., Zhong, Z., Gu, J., Nan, K., Zhu, M., & Miao, C. (2021). ets1 associates with KMT5A to participate in high glucose-mediated EndMT via upregulation of PFN2 expression in diabetic nephropathy. Molecular Medicine, 27(1), 1-19.

Lin, Z. Z., Ming, D. S., Chen, Y. B., Zhang, J. M., Chen, H. H., Jiang, J. J., & Zhang, Z. S. (2019). KMT5A promotes metastasis of clear cell renal cell carcinoma through reducing cadherin‑1 expression. Oncology Letters, 17(6), 4907-4913.

Liao, T., Wang, Y. J., Hu, J. Q., Wang, Y., Han, L. T., Ma, B., ... & Ji, Q. H. (2018). Histone methyltransferase KMT5A gene modulates oncogenesis and lipid metabolism of papillary thyroid cancer in vitro. Oncology reports, 39(5), 2185-2192.

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For research use only. Not intended for any clinical use.

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