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Mouse Anti-KMT5C Recombinant Antibody (CBXS-0036) (CBMAB-S3039-CQ)

This product is a mouse antibody that recognizes KMT5C. The antibody CBXS-0036 can be used for immunoassay techniques such as: IP.
See all KMT5C antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXS-0036
Antibody Isotype
IgG1
Application
IP

Basic Information

Immunogen
2-248 of 462 of SUV420H2 Protein
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.09% sodium azide
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Lysine Methyltransferase 5C
Introduction
SUV420H2 and the related enzyme SUV420H1 (MIM 610881) function as histone methyltransferases that specifically trimethylate nucleosomal histone H4 (see MIM 602822) on lysine-20 (K20) (Schotta et al., 2004 [PubMed 15145825]).
Entrez Gene ID
UniProt ID
Alternative Names
Lysine Methyltransferase 5C; Lysine (K)-Specific Methyltransferase 5C; Suppressor Of Variegation 4-20 Homolog 2; Lysine-Specific Methyltransferase 5C; Lysine N-Methyltransferase 5C; Su(Var)4-20 Homolog 2; Suv4-20h2;
Function
Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity (PubMed:24396869, PubMed:28114273).
In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes (PubMed:24396869).
H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5C is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (By similarity).
Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (PubMed:28114273).
May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity).
Biological Process
Chromatin organizationIEA:UniProtKB-KW
DNA repairManual Assertion Based On ExperimentIMP:UniProtKB
Histone H4-K20 dimethylationIEA:InterPro
Histone H4-K20 trimethylationManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of double-strand break repair via nonhomologous end joiningManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of isotype switchingISS:UniProtKB
Cellular Location
Nucleus
Chromosome
Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin (By similarity).

Montealegre, A. A. (2023). The role of KMT5C on EGFR inhibitor resistance in non-small cell lung cancer (Doctoral dissertation, Purdue University Graduate School).

Tong, Y., Wang, F., Li, S., Guo, W., Li, Q., Qian, Y., ... & Liu, Y. (2023). Histone methyltransferase KMT5C drives liver cancer progression and directs therapeutic response to PARP inhibitors. Hepatology, 10-1097.

Agredo, A., Pal, A., Son, J., Lanman, N. A., & Kasinski, A. L. (2023). Loss of the methyltransferase KMT5C drives resistance to tyrosine kinase inhibitors via H4K20me3 regulation in non-small cell lung cancer. Cancer Research, 83(7_Supplement), 4752-4752.

Pal, A. S., Agredo, A., Lanman, N. A., Son, J., Sohal, I. S., Bains, M., ... & Kasinski, A. L. (2022). Loss of KMT5C promotes EGFR inhibitor resistance in NSCLC via LINC01510-mediated upregulation of MET. Cancer Research, 82(8), 1534-1547.

Strickfaden, H., Missiaen, K., Knechtel, J. W., Hendzel, M. J., & Underhill, D. A. (2021). KMT5C encodes robust heterochromatin retention and liquid-like behavior using limited sequence features. bioRxiv, 2021-11.

Zhao, Q., Zhang, Z., Rong, W., Jin, W., Yan, L., Jin, W., ... & Pan, D. (2020). KMT5c modulates adipocyte thermogenesis by regulating Trp53 expression. Proceedings of the National Academy of Sciences, 117(36), 22413-22422.

Gao, J., Li, E., Liu, W., Yang, Q., Xie, C., Ai, J., ... & Wu, L. (2020). Circular RNA MYLK promotes hepatocellular carcinoma progression through the miR29a/KMT5C signaling pathway. OncoTargets and therapy, 8615-8627.

Strickfaden, H., Missiaen, K., Hendzel, M. J., & Underhill, D. A. (2019). KMT5C displays robust retention and liquid-like behavior in phase separated heterochromatin. BioRxiv, 776625.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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