Mouse Anti-KMT5C Recombinant Antibody (CBXS-0036) (CBMAB-S3039-CQ)

Name: Mouse Anti-KMT5C Recombinant Antibody (CBXS-0036)
SKU: CBMAB-S3039-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXS-0036

Application

Immunogen

2-248 of 462 of SUV420H2 Protein

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.09% sodium azide

Concentration

0.5 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Lysine Methyltransferase 5C

Introduction

SUV420H2 and the related enzyme SUV420H1 (MIM 610881) function as histone methyltransferases that specifically trimethylate nucleosomal histone H4 (see MIM 602822) on lysine-20 (K20) (Schotta et al., 2004 [PubMed 15145825]).

Entrez Gene ID

84787

UniProt ID

Q86Y97

Alternative Names

Lysine Methyltransferase 5C; Lysine (K)-Specific Methyltransferase 5C; Suppressor Of Variegation 4-20 Homolog 2; Lysine-Specific Methyltransferase 5C; Lysine N-Methyltransferase 5C; Su(Var)4-20 Homolog 2; Suv4-20h2;

Function

Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity (PubMed:24396869, PubMed:28114273).
In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes (PubMed:24396869).
H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5C is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (By similarity).
Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (PubMed:28114273).
May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity).

Biological Process

Chromatin organizationIEA:UniProtKB-KW
DNA repairManual Assertion Based On ExperimentIMP:UniProtKB
Histone H4-K20 dimethylationIEA:InterPro
Histone H4-K20 trimethylationManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of double-strand break repair via nonhomologous end joiningManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of isotype switchingISS:UniProtKB

Cellular Location

Nucleus
Chromosome
Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin (By similarity).

Montealegre, A. A. (2023). The role of KMT5C on EGFR inhibitor resistance in non-small cell lung cancer (Doctoral dissertation, Purdue University Graduate School).

Tong, Y., Wang, F., Li, S., Guo, W., Li, Q., Qian, Y., ... & Liu, Y. (2023). Histone methyltransferase KMT5C drives liver cancer progression and directs therapeutic response to PARP inhibitors. Hepatology, 10-1097.

Agredo, A., Pal, A., Son, J., Lanman, N. A., & Kasinski, A. L. (2023). Loss of the methyltransferase KMT5C drives resistance to tyrosine kinase inhibitors via H4K20me3 regulation in non-small cell lung cancer. Cancer Research, 83(7_Supplement), 4752-4752.

Pal, A. S., Agredo, A., Lanman, N. A., Son, J., Sohal, I. S., Bains, M., ... & Kasinski, A. L. (2022). Loss of KMT5C promotes EGFR inhibitor resistance in NSCLC via LINC01510-mediated upregulation of MET. Cancer Research, 82(8), 1534-1547.

Strickfaden, H., Missiaen, K., Knechtel, J. W., Hendzel, M. J., & Underhill, D. A. (2021). KMT5C encodes robust heterochromatin retention and liquid-like behavior using limited sequence features. bioRxiv, 2021-11.

Zhao, Q., Zhang, Z., Rong, W., Jin, W., Yan, L., Jin, W., ... & Pan, D. (2020). KMT5c modulates adipocyte thermogenesis by regulating Trp53 expression. Proceedings of the National Academy of Sciences, 117(36), 22413-22422.

Gao, J., Li, E., Liu, W., Yang, Q., Xie, C., Ai, J., ... & Wu, L. (2020). Circular RNA MYLK promotes hepatocellular carcinoma progression through the miR29a/KMT5C signaling pathway. OncoTargets and therapy, 8615-8627.

Strickfaden, H., Missiaen, K., Hendzel, M. J., & Underhill, D. A. (2019). KMT5C displays robust retention and liquid-like behavior in phase separated heterochromatin. BioRxiv, 776625.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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Isotype control

For research use only. Not intended for any clinical use.

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