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Rat Anti-KPNA3 Recombinant Antibody (3D10) (CBMAB-K1478-LY)

This product is antibody recognizes KPNA3. The antibody 3D10 immunoassay techniques such as: WB.
See all KPNA3 antibodies

Summary

Host Animal
Rat
Specificity
Human, Hamster, Mouse, Rat
Clone
3D10
Antibody Isotype
IgG2a
Application
WB

Basic Information

Immunogen
Human recombinant full-length Importin alpha3
Specificity
Human, Hamster, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
1 mg/mL
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Karyopherin Subunit Alpha 3
Introduction
The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. [provided by RefSeq, Jan 2009]
Entrez Gene ID
Human3839
Mouse16648
Rat361055
Hamster101824797
UniProt ID
HumanO00505
MouseO35344
RatQ56R18
HamsterA0A3Q0D085
Alternative Names
Karyopherin Subunit Alpha 3; Karyopherin Alpha 3 (Importin Alpha 4); Importin Subunit Alpha-4; Importin Alpha Q2; Importin Alpha 4; SRP1-Gamma; QIP2; Karyopherin Subunit Alpha-3; Importin Subunit Alpha-3;
Function
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9.
Biological Process
NLS-bearing protein import into nucleusManual Assertion Based On ExperimentIBA:GO_Central
Protein-containing complex assemblyManual Assertion Based On ExperimentTAS:ProtInc
Viral entry into host cellIEA:UniProtKB-KW
Viral penetration into host nucleusIEA:UniProtKB-KW
Cellular Location
Cytoplasm
Nucleus

Wang, X. W., Jiang, Y. H., Ye, W., Shao, C. F., Xie, J. J., & Li, X. (2023). SIRT1 promotes the progression and chemoresistance of colorectal cancer through the p53/miR-101/KPNA3 axis. Cancer Biology & Therapy, 24(1), 2235770.

Choi, J., Choi, J. H., Lee, H. W., Seo, D., Lkhagvasuren, G., Kim, J. W., ... & Lee, K. H. (2023). KPNA3 promotes epithelial-mesenchymal transition by regulating TGF-β and AKT signaling pathways in MDA-MB-231, a triple-negative breast cancer cell line. BMB reports, 56(2), 120.

Schob, C., Hempel, M., Safka Brozkova, D., Jiang, H., Kim, S. Y., Batzir, N. A., ... & Denecke, J. (2021). Dominant KPNA3 Mutations Cause Infantile‐Onset Hereditary Spastic Paraplegia. Annals of neurology, 90(5), 738-750.

Xie, Q., Wang, W., Li, L., Kan, Q., Fu, H., Geng, T., ... & Ye, J. (2021). Domain in Fiber-2 interacted with KPNA3/4 significantly affects the replication and pathogenicity of the highly pathogenic FAdV-4. Virulence, 12(1), 754-765.

Chen, X., Liu, Y., Zhang, Q., Liu, B., Cheng, Y., Zhang, Y., ... & Gen, H. (2021). Exosomal long non-coding RNA HOTTIP increases resistance of colorectal cancer cells to mitomycin via impairing MiR-214-mediated degradation of KPNA3. Frontiers in Cell and Developmental Biology, 8, 582723.

Chen, W. B., Zhang, L. X., Zhao, Y. K., Li, J., & Jiao, Y. (2020). C/EBPα-mediated transcriptional activation of miR-134-5p entails KPNA3 inhibition and modulates focal hypoxic-ischemic brain damage in neonatal rats. Brain Research Bulletin, 164, 350-360.

Hu, B., Cheng, J. W., Hu, J. W., Li, H., Ma, X. L., Tang, W. G., ... & Yang, X. R. (2019). KPNA3 confers sorafenib resistance to advanced hepatocellular carcinoma via TWIST regulated epithelial-mesenchymal transition. Journal of Cancer, 10(17), 3914.

Liu, T., Han, Z., Li, H., Zhu, Y., Sun, Z., & Zhu, A. (2018). LncRNA DLEU1 contributes to colorectal cancer progression via activation of KPNA3. Molecular cancer, 17, 1-13.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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