Mouse Anti-KRT8 Recombinant Antibody (4D12) (CBMAB-K2024-LY)

Name: Mouse Anti-KRT8 Recombinant Antibody (4D12)
SKU: CBMAB-K2024-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4D12

Application

IF, IHC, WB

Immunogen

KRT8 antibody was raised in mouse using a human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E. coli as the immunogen

Specificity

Human, Dog, Monkey

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

0.5 mg-1 mg/mL

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Keratin 8

Introduction

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Entrez Gene ID

Human	3856
Dog	486513
Monkey	700449

UniProt ID

Human

P05787

Alternative Names

Keratin 8; Type-II Keratin Kb8; Keratin 8; Type II; Cytokeratin-8; CK-8; CYK8; K8;

Function

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Cellular Location

Cytoplasm 2 Publications
Nucleus, nucleoplasm By Similarity
Nucleus matrix

Involvement in disease

Cirrhosis (CIRRH):
A liver disease characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper. Clinical features include abdomen swelling, jaundice and pulmonary hypertension.

PTM

Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Wang, D., Shang, Q., Mao, J., Gao, C., Wang, J., Wang, D., ... & Yang, L. (2023). Phosphorylation of KRT8 (keratin 8) by excessive mechanical load-activated PKN (protein kinase N) impairs autophagosome initiation and contributes to disc degeneration. Autophagy, 1-19.

Xiong, F., Guo, T., Wang, X., Wu, G., Liu, W., Wang, Q., ... & Chen, Y. (2022). Keratin 8 Is an Inflammation-Induced and Prognosis-Related Marker for Pancreatic Adenocarcinoma. Disease Markers, 2022.

Son, S., Baek, A., Lee, J. H., & Kim, D. E. (2022). Autophagosome–lysosome fusion is facilitated by plectin-stabilized actin and keratin 8 during macroautophagic process. Cellular and Molecular Life Sciences, 79(2), 95.

Baek, A., Son, S., Baek, Y. M., & Kim, D. E. (2021). KRT8 (keratin 8) attenuates necrotic cell death by facilitating mitochondrial fission-mediated mitophagy through interaction with PLEC (plectin). Autophagy, 17(12), 3939-3956.

Albaret, M. A., Paré, A., Malet, L., De Souza, G., Lavergne, E., Goga, D., ... & Saintigny, P. (2021). Combined proteomic and transcriptomic approaches reveal externalized keratin 8 as a potential therapeutic target involved in invasiveness of head and neck cancers. Translational Oncology, 14(1), 100878.

Miao, Q., Xu, Y., Yin, H., Zhang, H., & Ye, J. (2020). KRT8 phosphorylation regulates the epithelial‐mesenchymal transition in retinal pigment epithelial cells through autophagy modulation. Journal of Cellular and Molecular Medicine, 24(5), 3217-3228.

Golob-Schwarzl, N., Bettermann, K., Mehta, A. K., Kessler, S. M., Unterluggauer, J., Krassnig, S., ... & Haybaeck, J. (2019). High keratin 8/18 ratio predicts aggressive hepatocellular cancer phenotype. Translational oncology, 12(2), 256-268.

Xie, L., Dang, Y., Guo, J., Sun, X., Xie, T., Zhang, L., ... & Guo, X. (2019). High KRT8 expression independently predicts poor prognosis for lung adenocarcinoma patients. Genes, 10(1), 36.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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