Mouse Anti-KSHV ORF45 Recombinant Antibody (2D4A5) (CBMAB-AO687LY)

Name: Mouse Anti-KSHV ORF45 Recombinant Antibody (2D4A5)
SKU: CBMAB-AO687LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

2D4A5

Application

WB, ICC

Immunogen

Purified recombinant fragment of KSHV ORF45 expressed in E. coli.

Specificity

Virus

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Ascites

Preservative

0.03% sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

KSHV ORF45

Alternative Names

ORF45

Function

Prevents the establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of host interferon regulatory factor 7/IRF7, a transcription factor critical for the induction of interferons alpha and beta (PubMed:11943871, PubMed:20485504, PubMed:22787218).
Mechanistically, ORF45 competes with the associated IRF7 and inhibits its phosphorylation by IKBKE or TBK1 by acting as an alternative substrate (PubMed:11943871, PubMed:22787218).
Acts as an activator of the NLRP1 inflammasome via interaction with the N-terminal part of host NLRP1: interaction promotes translocation of the N-terminal part of NLRP1 into the nucleus, relieving autoinhibition of the NLRP1 inflammasome and leading to its activation (PubMed:35618833).
Also plays a role in promoting the late transcription and translation of viral lytic genes by constitutively activating host extracellular signal-regulated kinase (ERK)-p90 ribosomal S6 kinase/RPS6KA1 (PubMed:30842327).
In addition, supports the viral replication cycle by modulating host p53/TP53 signaling pathway (PubMed:34523970).
Interacts with host p53/TP53 and prevents its interaction with the deubiquitinase USP7, leading to sequestration of P53/TP53 in the host cytoplasm thereby diminishing its transcriptional activity (PubMed:34523970).

Biological Process

Symbiont-mediated perturbation of host innate immune response
Symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF7 activity

Cellular Location

Virion tegument
Host cytoplasm
Host nucleus
Host Golgi apparatus

PTM

Phosphorylated on Ser-41 and Ser-162 by host IKBKE and TBK1.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-KSHV ORF45 Recombinant Antibody (CBT2302) (CAT#: V2LY-0625-LY2041)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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