Mouse Anti-L3MBTL1 Recombinant Antibody (CBYJL-1035) (CBMAB-L0447-YJ)

Name: Mouse Anti-L3MBTL1 Recombinant Antibody (CBYJL-1035)
SKU: CBMAB-L0447-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJL-1035

Application

Immunogen

200-530 of 772 of L3MBTL1 Protein.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Preservative

0.09% Sodium Azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 200-530

More Infomation

Target

Full Name

L3Mbtl1 Histone Methyl-Lysine Binding Protein

Introduction

Gene of L3MBTL1 represents a polycomb group gene. L3MBTL1 functions to regulate gene activity, likely via chromatin modification. The encoded protein may also be necessary for mitosis. Alternatively spliced transcript variants encoding different isoforms have been found. Diseases associated with L3MBTL1 include Shwachman-Diamond Syndrome 1 and Brain Cancer. Among its related pathways are Gene Expression and Regulation of TP53 Activity.

Entrez Gene ID

26013

UniProt ID

Q9Y468

Alternative Names

H-L(3)MBT; L3MBTL; ZC2HC3; dJ138B7.3

Function

Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/H1-4 at 'Lys-26' (AC H1bK26me1 and AC H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.

Biological Process

Chromatin organizationManual Assertion Based On ExperimentIDA:UniProtKB
HemopoiesisManual Assertion Based On ExperimentIEP:UniProtKB
Heterochromatin assemblyManual Assertion Based On ExperimentIDA:ComplexPortal
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cell cycle1 PublicationNAS:UniProtKB
Regulation of megakaryocyte differentiationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of mitotic nuclear divisionManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Nucleus
Excluded from the nucleolus. Does not colocalizes with the PcG protein BMI1, suggesting that these two proteins do not belong to the same complex.

PTM

Ubiquitinated in a VCP/p97-dependent way following DNA damage, leading to its removal from DNA damage sites, promoting accessibility of H4K20me2 mark for DNA repair protein TP53BP1, which is then recruited to DNA damage sites.

Zhang, Z., Li, Y., Shi, R., Xu, S., Zhu, G., Cao, P., ... & Chen, J. (2023). L3MBTL1, a polycomb protein, promotes Osimertinib Acquired resistance through Epigenetic Regulation of DNA damage response in lung adenocarcinoma.

Yang, L., Li, Z., Liu, Y., Zhang, J. W., Wang, Y. X., Hu, J. J., ... & Sun, L. J. (2022). Influences of fresh and frozen embryo transfer on neonatal birthweight and the expression of imprinted genes PEG10/L3MBTL1 in placenta. Reproductive Biology, 22(3), 100665.

Wang, Y. X., Yue, L. F., Zhang, J. W., Xiong, Y. W., Hu, J. J., Wang, L. L., ... & Sun, L. J. (2021). Expression and DNA methylation status of the imprinted genes PEG10 and L3MBTL1 in the umbilical cord blood and placenta of the offspring of assisted reproductive technology. Reproductive Sciences, 28, 1133-1141.

Lu, J., Periz, G., Lu, Y. N., Tang, Q., Liu, Y., Zhang, T., ... & Wang, J. (2019). L3MBTL1 regulates ALS/FTD-associated proteotoxicity and quality control. Nature neuroscience, 22(6), 875-886.

Mao, W., Salzberg, A. C., Uchigashima, M., Hasegawa, Y., Hock, H., Watanabe, M., ... & Futai, K. (2018). Activity-induced regulation of synaptic strength through the chromatin reader L3mbtl1. Cell reports, 23(11), 3209-3222.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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