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Mouse Anti-LAPTM4B Recombinant Antibody (CBYJL-1189) (CBMAB-L0692-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Lysosomal Protein Transmembrane 4 Beta (LAPTM4B). The antibody can be used for immunoassay techniques, such as IHC.
See all LAPTM4B antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJL-1189
Antibody Isotype
IgG2b
Application
IHC

Basic Information

Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.2
Preservative
0.02% Sodium Azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
lysosomal-associated protein transmembrane 4B
Introduction
Among its related pathways are Lysosome. An important paralog of LAPTM4B is LAPTM4A.Required for optimal lysosomal function. Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation. LAPTM4B recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation. It plays a role as negative regulator of TGFB1 production in regulatory T cells. Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways.
Entrez Gene ID
55353
UniProt ID
Q86VI4
Alternative Names
LAPTM4beta; LC27
Function
Required for optimal lysosomal function (PubMed:21224396).
Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation (PubMed:25588945).
Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation (PubMed:25998567).
Plays a role as negative regulator of TGFB1 production in regulatory T cells (PubMed:26126825).
Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways (PubMed:26280656).
Biological Process
Endosome organizationManual Assertion Based On ExperimentIMP:UniProtKB
Endosome transport via multivesicular body sorting pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of lysosomal protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of transforming growth factor beta1 productionManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of lysosomal membrane permeabilityManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of lysosome organizationManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Endomembrane system
Late endosome membrane
Cell membrane
Cell projection
Lysosome membrane
Endosome membrane
Endosome, multivesicular body membrane
Endosome, multivesicular body lumen
PTM
Undergoes proteolytic cleavage following delivery to the lysosomes.
Ubiquitinated by NEDD4.

Wang, H., Wang, Q., Wu, Y., Lou, J., Zhu, S., & Xu, Y. (2023). Autophagy-related gene LAPTM4B promotes the progression of renal clear cell carcinoma and is associated with immunity. Frontiers in Pharmacology, 14, 1118217.

Ji, X., Ma, H., & Du, Y. (2022). Role and mechanism of action of LAPTM4B in EGFR‑mediated autophagy. Oncology Letters, 23(4), 1-6.

Liu, M., Yan, R., Wang, J., Yao, Z., Fan, X., & Zhou, K. (2022). LAPTM4B‐35 promotes cancer cell migration via stimulating integrin beta1 recycling and focal adhesion dynamics. Cancer Science, 113(6), 2022-2033.

Yin, Y., Fan, Y., Yu, G., & Du, Y. (2021). LAPTM4B promotes the progression of bladder cancer by stimulating cell proliferation and invasion. Oncology Letters, 22(5), 1-9.

Gu, S., Tan, J., Li, Q., Liu, S., Ma, J., Zheng, Y., ... & Yang, H. T. (2020). Downregulation of LAPTM4B contributes to the impairment of the autophagic flux via unopposed activation of mTORC1 signaling during myocardial ischemia/reperfusion injury. Circulation Research, 127(7), e148-e165.

Chu, C., Niu, X., Ou, X., & Hu, C. (2019). LAPTM4B knockdown increases the radiosensitivity of EGFR-overexpressing radioresistant nasopharyngeal cancer cells by inhibiting autophagy. OncoTargets and therapy, 12, 5661.

Wang, L., Meng, Y., & Zhang, Q. Y. (2019). LAPTM4B is a novel diagnostic and prognostic marker for lung adenocarcinoma and associated with mutant EGFR. BMC cancer, 19(1), 1-14.

Wang, F., Wu, H., Zhang, S., Lu, J., Lu, Y., Zhan, P., ... & Yin, Z. (2019). LAPTM4B facilitates tumor growth and induces autophagy in hepatocellular carcinoma. Cancer Management and Research, 2485-2497.

Hashemi, M., Bahari, G., Tabasi, F., Markowski, J., Małecki, A., Ghavami, S., & Łos, M. J. (2018). LAPTM4B gene polymorphism augments the risk of cancer: Evidence from an updated meta‐analysis. Journal of Cellular and Molecular Medicine, 22(12), 6396.

Wang, L., Meng, Y., Xu, J. J., & Zhang, Q. Y. (2018). The transcription factor AP4 promotes oncogenic phenotypes and cisplatin resistance by regulating LAPTM4B expression. Molecular Cancer Research, 16(5), 857-868.

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For research use only. Not intended for any clinical use.

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