Mouse Anti-LAPTM4B Recombinant Antibody (CBYJL-1189) (CBMAB-L0692-YJ)

Name: Mouse Anti-LAPTM4B Recombinant Antibody (CBYJL-1189)
SKU: CBMAB-L0692-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJL-1189

Application

IHC

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.2

Preservative

0.02% Sodium Azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

lysosomal-associated protein transmembrane 4B

Introduction

Among its related pathways are Lysosome. An important paralog of LAPTM4B is LAPTM4A.Required for optimal lysosomal function. Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation. LAPTM4B recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation. It plays a role as negative regulator of TGFB1 production in regulatory T cells. Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways.

Entrez Gene ID

55353

UniProt ID

Q86VI4

Alternative Names

LAPTM4beta; LC27

Function

Required for optimal lysosomal function (PubMed:21224396).
Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation (PubMed:25588945).
Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation (PubMed:25998567).
Plays a role as negative regulator of TGFB1 production in regulatory T cells (PubMed:26126825).
Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways (PubMed:26280656).

Biological Process

Endosome organizationManual Assertion Based On ExperimentIMP:UniProtKB
Endosome transport via multivesicular body sorting pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of lysosomal protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of transforming growth factor beta1 productionManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of lysosomal membrane permeabilityManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of lysosome organizationManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Endomembrane system
Late endosome membrane
Cell membrane
Cell projection
Lysosome membrane
Endosome membrane
Endosome, multivesicular body membrane
Endosome, multivesicular body lumen

PTM

Undergoes proteolytic cleavage following delivery to the lysosomes.
Ubiquitinated by NEDD4.

Wang, H., Wang, Q., Wu, Y., Lou, J., Zhu, S., & Xu, Y. (2023). Autophagy-related gene LAPTM4B promotes the progression of renal clear cell carcinoma and is associated with immunity. Frontiers in Pharmacology, 14, 1118217.

Ji, X., Ma, H., & Du, Y. (2022). Role and mechanism of action of LAPTM4B in EGFR‑mediated autophagy. Oncology Letters, 23(4), 1-6.

Liu, M., Yan, R., Wang, J., Yao, Z., Fan, X., & Zhou, K. (2022). LAPTM4B‐35 promotes cancer cell migration via stimulating integrin beta1 recycling and focal adhesion dynamics. Cancer Science, 113(6), 2022-2033.

Yin, Y., Fan, Y., Yu, G., & Du, Y. (2021). LAPTM4B promotes the progression of bladder cancer by stimulating cell proliferation and invasion. Oncology Letters, 22(5), 1-9.

Gu, S., Tan, J., Li, Q., Liu, S., Ma, J., Zheng, Y., ... & Yang, H. T. (2020). Downregulation of LAPTM4B contributes to the impairment of the autophagic flux via unopposed activation of mTORC1 signaling during myocardial ischemia/reperfusion injury. Circulation Research, 127(7), e148-e165.

Chu, C., Niu, X., Ou, X., & Hu, C. (2019). LAPTM4B knockdown increases the radiosensitivity of EGFR-overexpressing radioresistant nasopharyngeal cancer cells by inhibiting autophagy. OncoTargets and therapy, 12, 5661.

Wang, L., Meng, Y., & Zhang, Q. Y. (2019). LAPTM4B is a novel diagnostic and prognostic marker for lung adenocarcinoma and associated with mutant EGFR. BMC cancer, 19(1), 1-14.

Wang, F., Wu, H., Zhang, S., Lu, J., Lu, Y., Zhan, P., ... & Yin, Z. (2019). LAPTM4B facilitates tumor growth and induces autophagy in hepatocellular carcinoma. Cancer Management and Research, 2485-2497.

Hashemi, M., Bahari, G., Tabasi, F., Markowski, J., Małecki, A., Ghavami, S., & Łos, M. J. (2018). LAPTM4B gene polymorphism augments the risk of cancer: Evidence from an updated meta‐analysis. Journal of Cellular and Molecular Medicine, 22(12), 6396.

Wang, L., Meng, Y., Xu, J. J., & Zhang, Q. Y. (2018). The transcription factor AP4 promotes oncogenic phenotypes and cisplatin resistance by regulating LAPTM4B expression. Molecular Cancer Research, 16(5), 857-868.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
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For research use only. Not intended for any clinical use.

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