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Mouse Anti-LATS2 Recombinant Antibody (CBYJL-1219) (CBMAB-L0736-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Large Tumor Suppressor Kinase 2 (LATS2). The antibody can be used for immunoassay techniques, such as IP, WB.
See all LATS2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJL-1219
Antibody Isotype
IgG1
Application
IP, WB

Basic Information

Immunogen
N-terminus of human Lats2.
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
10 mM HEPES KOH, 150 mM NaCl, pH 7.5, 50% Glycerol
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
N-terminus

Target

Full Name
LARGE TUMOR SUPPRESSOR 2
Introduction
Large Tumor Suppressor Kinase 2 (LATS2) belongs to the LATS tumor suppressor family. LATS2 localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. LATS2 also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can play a role as a co-repressor of androgen-responsive gene expression.
Entrez Gene ID
UniProt ID
Alternative Names
KPM
Function
Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ.
Biological Process
Cell divisionIEA:UniProtKB-KW
G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentIDA:UniProtKB
Hippo signalingManual Assertion Based On ExperimentIDA:BHF-UCL
Hormone-mediated signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Intracellular signal transductionManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of canonical Wnt signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of cyclin-dependent protein serine/threonine kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of protein localization to nucleusISS:UniProtKB
Peptidyl-serine phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of apoptotic processManual Assertion Based On ExperimentIBA:GO_Central
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of organ growthManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transforming growth factor beta receptor signaling pathwayISS:UniProtKB
Cellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cytoplasm
Cytoplasm, cytoskeleton, spindle pole
Nucleus
Colocalizes with AURKA at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. Translocates to the nucleus upon mitotic stress by nocodazole treatment.
PTM
Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3/MST2.

Han, H., Nakaoka, H. J., Hofmann, L., Zhou, J. J., Yu, C., Zeng, L., ... & Wang, W. (2022). The hippo pathway kinases LATS1 and LATS2 attenuate cellular responses to heavy metals through phosphorylating MTF1. Nature cell biology, 24(1), 74-87.

Jin, D., Guo, J., Wu, Y., Yang, L., Wang, X., Du, J., ... & Sun, H. (2020). m 6 A demethylase ALKBH5 inhibits tumor growth and metastasis by reducing YTHDFs-mediated YAP expression and inhibiting miR-107/LATS2–mediated YAP activity in NSCLC. Molecular cancer, 19, 1-24.

Kim, E., Kang, J. G., Kang, M. J., Park, J. H., Kim, Y. J., Kweon, T. H., ... & Cho, J. W. (2020). O-GlcNAcylation on LATS2 disrupts the Hippo pathway by inhibiting its activity. Proceedings of the National Academy of Sciences, 117(25), 14259-14269.

Xie, S. C., Zhang, J. Q., Jiang, X. L., Hua, Y. Y., Xie, S. W., Qin, Y. A., & Yang, Y. J. (2020). LncRNA CRNDE facilitates epigenetic suppression of CELF2 and LATS2 to promote proliferation, migration and chemoresistance in hepatocellular carcinoma. Cell death & disease, 11(8), 676.

Tang, F., Gao, R., Jeevan-Raj, B., Wyss, C. B., Kalathur, R. K., Piscuoglio, S., ... & Christofori, G. (2019). LATS1 but not LATS2 represses autophagy by a kinase-independent scaffold function. Nature communications, 10(1), 5755.

Shi, Y., Geng, D., Zhang, Y., Zhao, M., Wang, Y., Jiang, Y., ... & Zhou, X. (2019). LATS2 inhibits malignant behaviors of glioma cells via inactivating YAP. Journal of Molecular Neuroscience, 68, 38-48.

Tsoi, M., Morin, M., Rico, C., Johnson, R. L., Paquet, M., Gévry, N., & Boerboom, D. (2019). Lats1 and Lats2 are required for ovarian granulosa cell fate maintenance. The FASEB Journal, 33(10), 10819.

Guo, C., Liang, C., Yang, J., Hu, H., Fan, B., & Liu, X. (2019). LATS2 inhibits cell proliferation and metastasis through the Hippo signaling pathway in glioma. Oncology reports, 41(5), 2753-2761.

Shi, X., Liu, Z., Liu, Z., Feng, X., Hua, F., Hu, X., ... & Nie, F. (2018). Long noncoding RNA PCAT6 functions as an oncogene by binding to EZH2 and suppressing LATS2 in non-small-cell lung cancer. EBioMedicine, 37, 177-187.

Furth, N., Pateras, I. S., Rotkopf, R., Vlachou, V., Rivkin, I., Schmitt, I., ... & Aylon, Y. (2018). LATS1 and LATS2 suppress breast cancer progression by maintaining cell identity and metabolic state. Life science alliance, 1(5).

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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