Mouse Anti-LCT Recombinant Antibody (CBYJL-1324) (CBMAB-L0909-YJ)

Name: Mouse Anti-LCT Recombinant Antibody (CBYJL-1324)
SKU: CBMAB-L0909-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJL-1324

Application

IHC

Immunogen

Normal jejunal epithelial brush border-enriched membranes from a non-secretor, blood group O.

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

LCT

Introduction

Lactase belongs to the glycosyl hydrolase 1 family of proteins. The encoded preproprotein is proteolytically processed to generate the mature enzyme. Lactase is integral to the plasma membrane and has both phlorizin hydrolase activity and lactase activity. Mutations in this gene are related to congenital lactase deficiency. Polymorphisms in this gene are associated with lactase persistence, in which intestinal lactase activity persists at childhood levels into adulthood.

Entrez Gene ID

3938

UniProt ID

P09848

Alternative Names

LAC; LPH; LPH1

Function

LPH splits lactose in the small intestine.

Biological Process

Carbohydrate metabolic processIEA:InterPro

Cellular Location

Apical cell membrane

Involvement in disease

Congenital lactase deficiency (COLACD):
Autosomal recessive, rare and severe gastrointestinal disorder. It is characterized by watery diarrhea in infants fed with breast milk or other lactose-containing formulas. An almost total lack of LCT activity is found in jejunal biopsy material of patients with congenital lactase deficiency. Opposite to congenital lactase deficiency, also known as lactose intolerance, is the most common enzyme deficiency worldwide. It is caused by developmental down-regulation of lactase activity during childhood or early adulthood. The decline of lactase activity is a normal physiological phenomenon; however, the majority of Northern Europeans have the ability to maintain lactase activity and digest lactose throughout life (lactase persistence). The down-regulation of lactase activity operates at the transcriptional level and it is associated with a noncoding variation in the MCM6 gene, located in the upstream vicinity of LCT.

Topology

Extracellular: 20-1882
Helical: 1883-1901
Cytoplasmic: 1902-1927

Németh, S., Kriegshäuser, G., Hovhannesyan, K., Hayrapetyan, H., Oberkanins, C., & Sarkisian, T. (2022). Very low frequency of the lactase persistence allele LCT-13910T in the Armenian population. Annals of Human Biology, 49(5-6), 260-262.

Nowak, J. K., Dybska, E., Dworacka, M., Tsikhan, N., Kononets, V., Bermagambetova, S., & Walkowiak, J. (2021). Ileal Lactase Expression Associates with Lactase Persistence Genotypes. Nutrients, 13(4), 1340.

Liu, Y. H., Wang, L., Zhang, Z., Otecko, N. O., Khederzadeh, S., Dai, Y., ... & Zhang, Y. P. (2021). Whole-Genome Sequencing Reveals Lactase Persistence Adaptation in European Dogs. Molecular Biology and Evolution, 38(11), 4884-4890.

Kuchay, R. A. H. (2020). New insights into the molecular basis of lactase non-persistence/persistence: A brief review. Drug Discoveries & Therapeutics, 14(1), 1-7.

Moghaddam, A. (2020). The Eurasian lactase persistence variant LCT-13910 C/T is associated with vitamin D levels in individuals living at high latitude, more so than exposure to sunlight. Journal of nutritional science, 9, e1.

Tomczonek-Moruś, J., Wojtasik, A., Zeman, K., Smolarz, B., & Bąk-Romaniszyn, L. (2019). 13910C> T and 22018G> A LCT gene polymorphisms in diagnosing hypolactasia in children. United European gastroenterology journal, 7(2), 210-216.

Abaturov, A. Y., Stepanov, Y. M., & Nikulina, A. A. (2019). Treatment of lactase deficiency in children’s obesity with genotype C/C 13910 of lactase gene. Wiadomości Lekarskie, 72(1), 17-21.

Bergholdt, H. K., Larsen, M. K., Varbo, A., Nordestgaard, B. G., & Ellervik, C. (2018). Lactase persistence, milk intake, hip fracture and bone mineral density: a study of 97 811 Danish individuals and a meta‐analysis. Journal of internal medicine, 284(3), 254-269.

Leseva, M. N., Grand, R. J., Klett, H., Boerries, M., Busch, H., Binder, A. M., & Michels, K. B. (2018). Differences in DNA methylation and functional expression in lactase persistent and non-persistent individuals. Scientific reports, 8(1), 5649.

Bergholdt, H. K. M., Nordestgaard, B. G., Varbo, A., & Ellervik, C. (2018). Lactase persistence, milk intake, and mortality in the Danish general population: a Mendelian randomization study. European journal of epidemiology, 33, 171-181.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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