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Mouse Anti-LFNG Recombinant Antibody (1B6) (CBMAB-L1219-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Lfng O-Fucosylpeptide 3-Beta-N-Acetylglucosaminyltransferase (LFNG). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all LFNG antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1B6
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
LFNG (NP_002295, 1 a.a. ~ 125 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: MTPGRCCLAADIQVETFIFT DGEDEALARH TGNVVITNCS AAHSRQALSC KMAVEYDRFI ESGRKWFCHV DDDNYVNLRA LLRLLASYPH TRDVYVGKPS LDRPIQAMER VSENKVRPVH FWFAT*.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-125

Target

Full Name
LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Introduction
LFNG is a member of the glycosyltransferase 31 gene family. Members of this gene family, which also includes the MFNG (GeneID: 4242) and RFNG (GeneID: 5986) genes, encode evolutionarily conserved glycosyltransferases that play a role in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, these proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. The protein encoded by this gene is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3.
Entrez Gene ID
3955
UniProt ID
Q8NES3
Alternative Names
SCDO3
Function
Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in inhibition of NOTCH1 activation by JAG1 and enhancement of NOTCH1 activation by DLL1 via an increase in its binding to DLL1 (By similarity).
Decreases the binding of JAG1 to NOTCH2 but not that of DLL1 (PubMed:11346656).
Essential mediator of somite segmentation and patterning (By similarity).
Biological Process
Animal organ morphogenesis1 PublicationNAS:UniProtKB
Compartment pattern specificationIEA:Ensembl
Marginal zone B cell differentiationISS:UniProtKB
Negative regulation of Notch signaling pathway involved in somitogenesisISS:UniProtKB
Ovarian follicle developmentIEA:Ensembl
Positive regulation of meiotic cell cycleIEA:Ensembl
Positive regulation of Notch signaling pathwayIEA:Ensembl
Positive regulation of protein bindingIEA:Ensembl
Regulation of Notch signaling pathwayISS:CAFA
Regulation of somitogenesisManual Assertion Based On ExperimentIMP:UniProtKB
SomitogenesisISS:UniProtKB
T cell differentiationISS:UniProtKB
Cellular Location
Golgi apparatus membrane
Involvement in disease
Spondylocostal dysostosis 3, autosomal recessive (SCDO3):
A condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life.
Topology
Cytoplasmic: 1-8
Helical: 9-29
Lumenal: 30-379
PTM
A soluble form may be derived from the membrane form by proteolytic processing.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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