Mouse Anti-LGALS12 Recombinant Antibody (CBYCL-256) (CBMAB-L0157-YC)

Provided herein is a Mouse monoclonal antibody against Human LGALS12. The antibody can be used for immunoassay techniques, such as WB, ICC, IHC-P, IHC-Fr, ELISA.
See all LGALS12 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBYCL-256

Antibody Isotype

IgG

Application

WB, ICC, IHC-P, IHC-Fr, ELISA

Basic Information

Immunogen

Galectin 12

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH7.4, with 0.02% sodium azide, 50% glycerol

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Galectin 12

Introduction

LGALS12 is a member of the galectin superfamily, a group of beta-galactoside-binding proteins with conserved carbohydrate recognition domains. The related mouse protein is a primary regulator of the early stages of adipose tissue development.

Entrez Gene ID

85329

UniProt ID

Q96DT0

Alternative Names

GAL12; GRIP1

Function

Binds lactose. May participate in the apoptosis of adipocytes.

Biological Process

Intrinsic apoptotic signaling pathwayManual Assertion Based On ExperimentIDA:MGI
Lymphatic endothelial cell migrationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of fat cell differentiationIEA:InterPro
Regulation of lipid catabolic processIEA:InterPro

Cellular Location

Nucleus

References

Wu, W., Yin, Y., Huang, J., Yang, R., Li, Q., Pan, J., & Zhang, J. (2023). CRISPR/Cas9-meditated gene knockout in pigs proves that LGALS12 deficiency suppresses the proliferation and differentiation of porcine adipocytes. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 159424.

Wu, W., Zhang, D., Yin, Y., Ji, M., Xu, K., Huang, X., ... & Zhang, J. (2019). Comprehensive transcriptomic view of the role of the LGALS12 gene in porcine subcutaneous and intramuscular adipocytes. BMC genomics, 20, 1-13.

Wu, W., Yin, Y., Xu, K., Peng, Y., & Zhang, J. (2018). Knockdown of LGALS12 inhibits porcine adipocyte adipogenesis via PKA–Erk1/2 signaling pathway. Acta Biochimica et Biophysica Sinica, 50(10), 960-967.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Second antibody and isotype control

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