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Rat Anti-LGR4 Recombinant Antibody (BBX-1H1) (CBMAB-L1336-YJ)

Provided herein is a Rat monoclonal antibody, which binds to Leucine Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4). The antibody can be used for immunoassay techniques, such as FC, ELISA.
See all LGR4 antibodies

Summary

Host Animal
Rat
Specificity
Human
Clone
BBX-1H1
Antibody Isotype
IgG2a, κ
Application
FC, ELISA

Basic Information

Immunogen
anti-human LGR4.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
leucine-rich repeat-containing G protein-coupled receptor 4
Introduction
LGR4 is a G-protein coupled receptor that binds R-spondins and activates the Wnt signaling pathway. This Wnt signaling pathway activation is essential for proper development of many organs of the body.
Entrez Gene ID
55366
UniProt ID
Q9BXB1
Alternative Names
GPR48; BNMD17
Function
Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye. May also act as a receptor for norrin (NDP), such results however require additional confirmation in vivo. Required during spermatogenesis to activate the Wnt signaling pathway in peritubular myoid cells. Required for the maintenance of intestinal stem cells and Paneth cell differentiation in postnatal intestinal crypts. Acts as a regulator of bone formation and remodeling. Involved in kidney development; required for maintaining the ureteric bud in an undifferentiated state. Involved in the development of the anterior segment of the eye. Required during erythropoiesis. Also acts as a negative regulator of innate immunity by inhibiting TLR2/TLR4 associated pattern-recognition and proinflammatory cytokine production. Plays an important role in regulating the circadian rhythms of plasma lipids, partially through regulating the rhythmic expression of MTTP (By similarity).
Biological Process
Activation of adenylate cyclase activityManual Assertion Based On ExperimentIBA:GO_Central
Adenylate cyclase-activating G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Bone mineralizationISS:UniProtKB
Bone remodelingISS:UniProtKB
Canonical Wnt signaling pathway involved in metanephric kidney developmentIEA:Ensembl
Cell differentiation involved in metanephros developmentIEA:Ensembl
Circadian regulation of gene expressionISS:UniProtKB
Digestive tract developmentIEA:Ensembl
Epithelial cell proliferation involved in renal tubule morphogenesisIEA:Ensembl
Hair follicle developmentIEA:Ensembl
Hormone-mediated signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Innate immune responseIEA:UniProtKB-KW
Intestinal stem cell homeostasisIEA:Ensembl
Male genitalia developmentIEA:Ensembl
Metanephric glomerulus developmentIEA:Ensembl
Metanephric nephron tubule morphogenesisIEA:Ensembl
Negative regulation of cold-induced thermogenesisBy SimilarityISS:YuBioLab
Negative regulation of cytokine productionISS:UniProtKB
Negative regulation of toll-like receptor signaling pathwayISS:UniProtKB
Negative regulation of transcription, DNA-templatedIEA:Ensembl
Osteoblast differentiationISS:UniProtKB
Positive regulation of branching involved in ureteric bud morphogenesisIEA:Ensembl
Positive regulation of canonical Wnt signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription, DNA-templatedIEA:Ensembl
SpermatogenesisISS:UniProtKB
Cellular Location
Cell membrane
Involvement in disease
Osteoporosis (OSTEOP):
A systemic skeletal disorder characterized by decreased bone mass and deterioration of bone microarchitecture without alteration in the composition of bone. The result is fragile bones and an increased risk of fractures, even after minimal trauma. Osteoporosis is a chronic condition of multifactorial etiology and is usually clinically silent until a fracture occurs.
Topology
Extracellular: 25-544
Helical: 545-565
Cytoplasmic: 566-575
Helical: 576-596
Extracellular: 597-620
Helical: 621-641
Cytoplasmic: 642-661
Helical: 662-682
Extracellular: 683-703
Helical: 704-724
Cytoplasmic: 725-756
Helical: 757-777
Extracellular: 778-783
Helical: 784-804
Cytoplasmic: 805-951

Saponara, E., Penno, C., Orsini, V., Wang, Z. Y., Fischer, A., Aebi, A., ... & Ruffner, H. (2023). Loss of Hepatic Leucine-Rich Repeat-Containing G-Protein Coupled Receptors 4 and 5 Promotes Nonalcoholic Fatty Liver Disease. The American Journal of Pathology, 193(2), 161-181.

Shimamura, M., Hayashi, H., Ju, N., Yoshida, S., Baba, S., Sasaki, T., ... & Nakagami, H. (2023). R-spondin 3/LGR4 (Leucine-Rich Repeat-Containing G Protein-Coupled Receptor 4) Axis Is a Novel Inflammatory and Neurite Outgrowth Signaling System in the Ischemic Brain in Mice. Stroke, 54(6), 1606-1615.

Bi, Y., Zhang, L., Song, Y., Sun, L., Mulholland, M. W., Yin, Y., & Zhang, W. (2023). Rspo2-LGR4 exacerbates hepatocellular carcinoma progression via activation of Wnt/β-catenin signaling pathway. Gastroenterología y Hepatología.

Filipowska, J., Kondegowda, N. G., Leon-Rivera, N., Dhawan, S., & Vasavada, R. C. (2022). LGR4, a G protein-coupled receptor with a systemic role: from development to metabolic regulation. Frontiers in Endocrinology, 13, 867001.

Carrillo-López, N., Martínez-Arias, L., Alonso-Montes, C., Martín-Carro, B., Martín-Vírgala, J., Ruiz-Ortega, M., ... & Panizo, S. (2021). The receptor activator of nuclear factor κΒ ligand receptor leucine-rich repeat-containing G-protein-coupled receptor 4 contributes to parathyroid hormone-induced vascular calcification. Nephrology Dialysis Transplantation, 36(4), 618-631.

Lee, Y. H., Sharma, A. R., Jagga, S., Lee, S. S., & Nam, J. S. (2021). Differential expression patterns of rspondin family and leucine-rich repeat-containing G-protein coupled receptors in chondrocytes and osteoblasts. Cell Journal (Yakhteh), 22(4), 437.

Cui, J., Toh, Y., Park, S., Yu, W., Tu, J., Wu, L., ... & Liu, Q. J. (2021). Drug conjugates of antagonistic R-spondin 4 mutant for simultaneous targeting of Leucine-rich repeat-containing G protein-coupled receptors 4/5/6 for cancer treatment. Journal of Medicinal Chemistry, 64(17), 12572-12581.

Liu, X., & Xu, X. (2018). MicroRNA-137 dysregulation predisposes to osteoporotic fracture by impeding ALP activity and expression via suppression of leucine-rich repeat-containing G-protein-coupled receptor 4 expression. International journal of molecular medicine, 42(2), 1026-1033.

Da Silva, F., Massa, F., Motamedi, F. J., Vidal, V., Rocha, A. S., Gregoire, E. P., ... & Schedl, A. (2018). Myocardial-specific R-spondin3 drives proliferation of the coronary stems primarily through the Leucine Rich Repeat G Protein coupled receptor LGR4. Developmental biology, 441(1), 42-51.

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For research use only. Not intended for any clinical use.

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