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Mouse Anti-LIMK1 Recombinant Antibody (A905) (CBMAB-AP11496LY)


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Summary

Host Animal
Mouse
Specificity
Mouse
Clone
A905
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
A synthetic peptide of human LIMK1
Specificity
Mouse
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
Affinity purity
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID
16885
UniProt ID
P53668
Alternative Names
LIMK
Function
A0547
Biological Process
Actin cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Fc-gamma receptor signaling pathway involved in phagocytosisTAS:Reactome
Negative regulation of ubiquitin-protein transferase activityManual Assertion Based On ExperimentIDA:BHF-UCL
Nervous system developmentManual Assertion Based On ExperimentTAS:ProtInc
Positive regulation of actin filament bundle assemblyManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of axon extensionISS:UniProtKB
Positive regulation of stress fiber assemblyManual Assertion Based On ExperimentIBA:GO_Central
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Rho protein signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Cytoplasm
Nucleus
Cytoplasm, cytoskeleton
Cell projection, lamellipodium
Predominantly found in the cytoplasm. Localizes in the lamellipodium in a CDC42BPA, CDC42BPB and FAM89B/LRAP25-dependent manner.
Involvement in disease
LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
PTM
Autophosphorylated. Phosphorylated on Thr-508 by ROCK1 and PAK1, resulting in activation. Phosphorylated by PAK4 which increases the ability of LIMK1 to phosphorylate cofilin. Phosphorylated at Ser-323 by MAPKAPK2 during activation of VEGFA-induced signaling, which results in activation of LIMK1 and promotion of actin reorganization, cell migration, and tubule formation of endothelial cells. Dephosphorylated and inactivated by SSH1. Phosphorylated by CDC42BP.
Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM (By similarity).
More Infomation

Villalonga, E., Mosrin, C., Normand, T., Girardin, C., Serrano, A., Žunar, B., ... & Vallée, B. (2023). LIM Kinases, LIMK1 and LIMK2, Are Crucial Node Actors of the Cell Fate: Molecular to Pathological Features. Cells, 12(5), 805.

Collins, R., Lee, H., Jones, D. H., Elkins, J. M., Gillespie, J. A., Thomas, C., ... & Foley, D. W. (2022). Comparative analysis of small-molecule LIMK1/2 inhibitors: chemical synthesis, biochemistry, and cellular activity. Journal of Medicinal Chemistry, 65(20), 13705-13713.

Kang, X., Li, W., Liu, W., Liang, H., Deng, J., Wong, C. C., ... & Ng, E. K. W. (2021). LIMK1 promotes peritoneal metastasis of gastric cancer and is a therapeutic target. Oncogene, 40(19), 3422-3433.

Lu, G., Zhou, Y., Zhang, C., & Zhang, Y. (2021). Upregulation of LIMK1 is correlated with poor prognosis and immune infiltrates in lung adenocarcinoma. Frontiers in genetics, 12, 671585.

Zhang, M., Wang, R., Tian, J., Song, M., Zhao, R., Liu, K., ... & Lee, M. H. (2021). Targeting LIMK1 with luteolin inhibits the growth of lung cancer in vitro and in vivo. Journal of cellular and molecular medicine, 25(12), 5560-5571.

Li, Z. F., Yao, Y. D., Zhao, Y. Y., Liu, Y., Liu, Z. H., Hu, P., & Zhu, Z. R. (2020). Effects of PAK4/LIMK1/Cofilin‐1 signaling pathway on proliferation, invasion, and migration of human osteosarcoma cells. Journal of clinical laboratory analysis, 34(9), e23362.

Salah, E., Chatterjee, D., Beltrami, A., Tumber, A., Preuss, F., Canning, P., ... & Mathea, S. (2019). Lessons from LIMK1 enzymology and their impact on inhibitor design. Biochemical Journal, 476(21), 3197-3209.

Henderson, B. W., Greathouse, K. M., Ramdas, R., Walker, C. K., Rao, T. C., Bach, S. V., ... & Herskowitz, J. H. (2019). Pharmacologic inhibition of LIMK1 provides dendritic spine resilience against β-amyloid. Science signaling, 12(587), eaaw9318.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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