Mouse Anti-LIMK1 Recombinant Antibody (A905) (CBMAB-AP11496LY)

Name: Mouse Anti-LIMK1 Recombinant Antibody (A905)
SKU: CBMAB-AP11496LY
Rating: 5 (1 reviews)

See all LIMK1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

A905

Application

Immunogen

A synthetic peptide of human LIMK1

Specificity

Mouse

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

Affinity purity

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

LIMK1

Entrez Gene ID

16885

UniProt ID

P53668

Alternative Names

LIMK

Function

A0547

Biological Process

Actin cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Fc-gamma receptor signaling pathway involved in phagocytosisTAS:Reactome
Negative regulation of ubiquitin-protein transferase activityManual Assertion Based On ExperimentIDA:BHF-UCL
Nervous system developmentManual Assertion Based On ExperimentTAS:ProtInc
Positive regulation of actin filament bundle assemblyManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of axon extensionISS:UniProtKB
Positive regulation of stress fiber assemblyManual Assertion Based On ExperimentIBA:GO_Central
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Rho protein signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Cytoplasm
Nucleus
Cytoplasm, cytoskeleton
Cell projection, lamellipodium
Predominantly found in the cytoplasm. Localizes in the lamellipodium in a CDC42BPA, CDC42BPB and FAM89B/LRAP25-dependent manner.

Involvement in disease

LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.

PTM

Autophosphorylated. Phosphorylated on Thr-508 by ROCK1 and PAK1, resulting in activation. Phosphorylated by PAK4 which increases the ability of LIMK1 to phosphorylate cofilin. Phosphorylated at Ser-323 by MAPKAPK2 during activation of VEGFA-induced signaling, which results in activation of LIMK1 and promotion of actin reorganization, cell migration, and tubule formation of endothelial cells. Dephosphorylated and inactivated by SSH1. Phosphorylated by CDC42BP.
Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM (By similarity).

Villalonga, E., Mosrin, C., Normand, T., Girardin, C., Serrano, A., Žunar, B., ... & Vallée, B. (2023). LIM Kinases, LIMK1 and LIMK2, Are Crucial Node Actors of the Cell Fate: Molecular to Pathological Features. Cells, 12(5), 805.

Collins, R., Lee, H., Jones, D. H., Elkins, J. M., Gillespie, J. A., Thomas, C., ... & Foley, D. W. (2022). Comparative analysis of small-molecule LIMK1/2 inhibitors: chemical synthesis, biochemistry, and cellular activity. Journal of Medicinal Chemistry, 65(20), 13705-13713.

Kang, X., Li, W., Liu, W., Liang, H., Deng, J., Wong, C. C., ... & Ng, E. K. W. (2021). LIMK1 promotes peritoneal metastasis of gastric cancer and is a therapeutic target. Oncogene, 40(19), 3422-3433.

Lu, G., Zhou, Y., Zhang, C., & Zhang, Y. (2021). Upregulation of LIMK1 is correlated with poor prognosis and immune infiltrates in lung adenocarcinoma. Frontiers in genetics, 12, 671585.

Zhang, M., Wang, R., Tian, J., Song, M., Zhao, R., Liu, K., ... & Lee, M. H. (2021). Targeting LIMK1 with luteolin inhibits the growth of lung cancer in vitro and in vivo. Journal of cellular and molecular medicine, 25(12), 5560-5571.

Li, Z. F., Yao, Y. D., Zhao, Y. Y., Liu, Y., Liu, Z. H., Hu, P., & Zhu, Z. R. (2020). Effects of PAK4/LIMK1/Cofilin‐1 signaling pathway on proliferation, invasion, and migration of human osteosarcoma cells. Journal of clinical laboratory analysis, 34(9), e23362.

Salah, E., Chatterjee, D., Beltrami, A., Tumber, A., Preuss, F., Canning, P., ... & Mathea, S. (2019). Lessons from LIMK1 enzymology and their impact on inhibitor design. Biochemical Journal, 476(21), 3197-3209.

Henderson, B. W., Greathouse, K. M., Ramdas, R., Walker, C. K., Rao, T. C., Bach, S. V., ... & Herskowitz, J. H. (2019). Pharmacologic inhibition of LIMK1 provides dendritic spine resilience against β-amyloid. Science signaling, 12(587), eaaw9318.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-LIMK1 Recombinant Antibody (A905)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-LIMK1 Recombinant Antibody (2E9) (CAT#: CBMAB-L1604-YJ)

Mouse Anti-LIMK1 Recombinant Antibody (CBYJL-1718) (CAT#: CBMAB-L1608-YJ)

Mouse Anti-LIMK1 Recombinant Antibody (3H6) (CAT#: CBMAB-L1607-YJ)

Mouse Anti-LIMK1 Recombinant Antibody (CBYJL-2605) (CAT#: CBMAB-L1602-YJ)

Mouse Anti-LIMK1 Recombinant Antibody (CBYJL-1714) (CAT#: CBMAB-L1599-YJ)

Mouse Anti-LIMK1 Recombinant Antibody (CBYCL-337) (CAT#: CBMAB-L0238-YC)

Mouse Anti-LIMK1 Recombinant Antibody (CBYJL-1717) (CAT#: CBMAB-L1605-YJ)

Mouse Anti-LIMK1 Recombinant Antibody (CBYJL-1720) (CAT#: CBMAB-L1612-YJ)

Mouse Anti-LIMK1 Recombinant Antibody (CBYJL-1719) (CAT#: CBMAB-L1610-YJ)

Mouse Anti-LIMK1 Recombinant Antibody (CBYJL-1723) (CAT#: CBMAB-L1616-YJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry