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Mouse Anti-LPCAT2 Recombinant Antibody (CBYJL-1917) (CBMAB-L1951-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Lysophosphatidylcholine Acyltransferase 2 (LPCAT2). The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all LPCAT2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJL-1917
Antibody Isotype
IgG
Application
WB, IP, IF, ELISA

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
lysophosphatidylcholine acyltransferase 2
Introduction
LPCAT2 is a member of the lysophospholipid acyltransferase family. LPCAT2 may function in two ways: to catalyze the biosynthesis of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) from 1-O-alkyl-sn-glycero-3-phosphocholine, and to catalyze the synthesis of glycerophospholipid precursors from arachidonyl-CoA and lysophosphatidylcholine. LPCAT2 may function in membrane biogenesis and production of platelet-activating factor in inflammatory cells. LPCAT2 may localize to the endoplasmic reticulum and the Golgi.
Entrez Gene ID
54947
UniProt ID
Q7L5N7
Alternative Names
AYTL1; AGPAT11; LysoPAFAT; lysophosphatidylcholine acyltransferase 2; 1-AGP acyltransferase 11; 1-AGPAT 11; 1-acylglycerol-3-phosphate O-acyltransferase 11; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; LPAAT-alpha; LPC acyltransferase 2; LPCAT-2; acetyl-CoA:lyso-PAF acetyltransferase; acetyl-CoA:lyso-platelet-activating factor acetyltransferase; acyl-CoA:lysophosphatidylcholine acyltransferase 2; acyltransferase-like 1; lyso-PAF acetyltransferase; lyso-platelet-activating factor (PAF) acetyltransferase; lysoPC acyltransferase 2; lysophosphatidic acid acyltransferase alpha; EC 2.3.1.23; EC 2.3.1.51; EC 2.3.1.67
Function
Exhibits both acyltransferase and acetyltransferase activities (PubMed:17182612, PubMed:20363836, PubMed:21498505).
Catalyzes the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (PubMed:21498505).
Catalyzes the conversion 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed:20363836).
Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF) (PubMed:17182612).
Also converts lyso-PAF to 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine (PC), a major component of cell membranes and a PAF precursor (By similarity).
Under resting conditions, acyltransferase activity is preferred (By similarity).
Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases (By similarity).
Involved in the regulation of lipid droplet number and size (PubMed:25491198).
Biological Process
Membrane organizationISS:UniProtKB
Phosphatidylcholine acyl-chain remodelingManual Assertion Based On ExperimentIDA:UniProtKB
Platelet activating factor biosynthetic processISS:UniProtKB
Cellular Location
Endoplasmic reticulum membrane
Golgi apparatus membrane
Cell membrane
Lipid droplet
Topology
Cytoplasmic: 1-57
Helical: 58-78
Lumenal: 79-544

Merkel, M., Goebel, B., Boll, M., Adhikari, A., Maurer, V., Steinhilber, D., & Culmsee, C. (2023). Mitochondrial Reactive Oxygen Species Formation Determines ACSL4/LPCAT2-Mediated Ferroptosis. Antioxidants, 12(8), 1590.

Wang, Q., Zhou, S., Hu, X., Wang, X., Wu, X., Huai, Z., ... & Li, S. (2023). Circadian Genes MBOAT2/CDA/LPCAT2/B4GALT5 in the Metabolic Pathway Serve as New Biomarkers of PACA Prognosis and Immune Infiltration. Life, 13(5), 1116.

Poloamina, V. I., Abate, W., Fejer, G., & Jackson, S. K. (2022). Possible regulation of Toll-like receptor 4 by lysine acetylation through LPCAT2 activity in RAW264. 7 cells. Bioscience Reports, 42(7), BSR20220251.

Poloamina, V. I. (2021). Regulation of the expression and lysine acetylation of pro-inflammatory molecules by lipid-modifying enzyme (LPCAT2) in RAW264. 7 cells (Doctoral dissertation, University of Plymouth).

Watanabe, H., Miyake, K., Matsuoka, T., Kojima, R., Sakurai, D., Masuyama, K., & Yamagata, Z. (2021). LPCAT2 methylation, a novel biomarker for the severity of cedar pollen allergic rhinitis in Japan. American Journal of Rhinology & Allergy, 35(5), 631-639.

Abate, W., Alrammah, H., Kiernan, M., Tonks, A. J., & Jackson, S. K. (2020). Lysophosphatidylcholine acyltransferase 2 (LPCAT2) co-localises with TLR4 and regulates macrophage inflammatory gene expression in response to LPS. Scientific Reports, 10(1), 10355.

Xiao, X., Zhuang, X., Xu, C., Chen, H., Zhu, W., Pang, C., & Zhang, M. (2020). ROBO4 deletion ameliorates PAF-mediated skin inflammation via regulating the mRNA translation efficiency of LPCAT1/LPCAT2 and the expression of PAF receptor. International Journal of Biological Sciences, 16(6), 1086.

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For research use only. Not intended for any clinical use.

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