Mouse Anti-LPCAT2 Recombinant Antibody (CBYJL-1917) (CBMAB-L1951-YJ)

Name: Mouse Anti-LPCAT2 Recombinant Antibody (CBYJL-1917)
SKU: CBMAB-L1951-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJL-1917

Application

WB, IP, IF, ELISA

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

lysophosphatidylcholine acyltransferase 2

Introduction

LPCAT2 is a member of the lysophospholipid acyltransferase family. LPCAT2 may function in two ways: to catalyze the biosynthesis of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) from 1-O-alkyl-sn-glycero-3-phosphocholine, and to catalyze the synthesis of glycerophospholipid precursors from arachidonyl-CoA and lysophosphatidylcholine. LPCAT2 may function in membrane biogenesis and production of platelet-activating factor in inflammatory cells. LPCAT2 may localize to the endoplasmic reticulum and the Golgi.

Entrez Gene ID

54947

UniProt ID

Q7L5N7

Alternative Names

AYTL1; AGPAT11; LysoPAFAT; lysophosphatidylcholine acyltransferase 2; 1-AGP acyltransferase 11; 1-AGPAT 11; 1-acylglycerol-3-phosphate O-acyltransferase 11; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; LPAAT-alpha; LPC acyltransferase 2; LPCAT-2; acetyl-CoA:lyso-PAF acetyltransferase; acetyl-CoA:lyso-platelet-activating factor acetyltransferase; acyl-CoA:lysophosphatidylcholine acyltransferase 2; acyltransferase-like 1; lyso-PAF acetyltransferase; lyso-platelet-activating factor (PAF) acetyltransferase; lysoPC acyltransferase 2; lysophosphatidic acid acyltransferase alpha; EC 2.3.1.23; EC 2.3.1.51; EC 2.3.1.67

Function

Exhibits both acyltransferase and acetyltransferase activities (PubMed:17182612, PubMed:20363836, PubMed:21498505).
Catalyzes the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (PubMed:21498505).
Catalyzes the conversion 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed:20363836).
Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF) (PubMed:17182612).
Also converts lyso-PAF to 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine (PC), a major component of cell membranes and a PAF precursor (By similarity).
Under resting conditions, acyltransferase activity is preferred (By similarity).
Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases (By similarity).
Involved in the regulation of lipid droplet number and size (PubMed:25491198).

Biological Process

Membrane organizationISS:UniProtKB
Phosphatidylcholine acyl-chain remodelingManual Assertion Based On ExperimentIDA:UniProtKB
Platelet activating factor biosynthetic processISS:UniProtKB

Cellular Location

Endoplasmic reticulum membrane
Golgi apparatus membrane
Cell membrane
Lipid droplet

Topology

Cytoplasmic: 1-57
Helical: 58-78
Lumenal: 79-544

Merkel, M., Goebel, B., Boll, M., Adhikari, A., Maurer, V., Steinhilber, D., & Culmsee, C. (2023). Mitochondrial Reactive Oxygen Species Formation Determines ACSL4/LPCAT2-Mediated Ferroptosis. Antioxidants, 12(8), 1590.

Wang, Q., Zhou, S., Hu, X., Wang, X., Wu, X., Huai, Z., ... & Li, S. (2023). Circadian Genes MBOAT2/CDA/LPCAT2/B4GALT5 in the Metabolic Pathway Serve as New Biomarkers of PACA Prognosis and Immune Infiltration. Life, 13(5), 1116.

Poloamina, V. I., Abate, W., Fejer, G., & Jackson, S. K. (2022). Possible regulation of Toll-like receptor 4 by lysine acetylation through LPCAT2 activity in RAW264. 7 cells. Bioscience Reports, 42(7), BSR20220251.

Poloamina, V. I. (2021). Regulation of the expression and lysine acetylation of pro-inflammatory molecules by lipid-modifying enzyme (LPCAT2) in RAW264. 7 cells (Doctoral dissertation, University of Plymouth).

Watanabe, H., Miyake, K., Matsuoka, T., Kojima, R., Sakurai, D., Masuyama, K., & Yamagata, Z. (2021). LPCAT2 methylation, a novel biomarker for the severity of cedar pollen allergic rhinitis in Japan. American Journal of Rhinology & Allergy, 35(5), 631-639.

Abate, W., Alrammah, H., Kiernan, M., Tonks, A. J., & Jackson, S. K. (2020). Lysophosphatidylcholine acyltransferase 2 (LPCAT2) co-localises with TLR4 and regulates macrophage inflammatory gene expression in response to LPS. Scientific Reports, 10(1), 10355.

Xiao, X., Zhuang, X., Xu, C., Chen, H., Zhu, W., Pang, C., & Zhang, M. (2020). ROBO4 deletion ameliorates PAF-mediated skin inflammation via regulating the mRNA translation efficiency of LPCAT1/LPCAT2 and the expression of PAF receptor. International Journal of Biological Sciences, 16(6), 1086.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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