Rabbit Anti-LRSAM1 Recombinant Antibody (CBYCL-496) (CBMAB-L0397-YC)

Name: Rabbit Anti-LRSAM1 Recombinant Antibody (CBYCL-496)
SKU: CBMAB-L0397-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBYCL-496

Application

Specificity

Human, Mouse, Rat, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

leucine rich repeat and sterile alpha motif containing 1

Introduction

LRSAM1 is a ring finger protein involved in a variety of functions, including regulation of signaling pathways and cell adhesion, mediation of self-ubiquitylation, and involvement in cargo sorting during receptor endocytosis. Mutations in this gene have been associated with Charcot-Marie-Tooth disease. Multiple transcript variants encoding different isoforms have been identified for this gene.

Entrez Gene ID

Human	90678
Mouse	227738
Rat	311866
Monkey	700704

UniProt ID

Human	Q6UWE0
Mouse	Q80ZI6
Rat	D3ZI42
Monkey	G8F4M7

Alternative Names

CMT2P; RIFLE; TAL

Function

E3 ubiquitin-protein ligase that mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic (EGF receptors) and exocytic (HIV-1 viral proteins) cargos (PubMed:15256501).
Bacterial recognition protein that defends the cytoplasm from invasive pathogens (PubMed:23245322).
Localizes to several intracellular bacterial pathogens and generates the bacteria-associated ubiquitin signal leading to autophagy-mediated intracellular bacteria degradation (xenophagy) (PubMed:23245322, PubMed:25484098).

Biological Process

AutophagyIEA:UniProtKB-KW
Negative regulation of endocytosisManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of autophagosome assemblyManual Assertion Based On ExperimentIMP:GO_Central
Positive regulation of xenophagyManual Assertion Based On ExperimentIMP:GO_Central
Protein autoubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Protein polyubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Ubiquitin-dependent endocytosisManual Assertion Based On ExperimentIDA:UniProtKB
Viral buddingManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cytoplasm
Displays a punctuate distribution and localizes to a submembranal ring (PubMed:15256501).
Localizes to intracellular bacterial pathogens (PubMed:23245322).

Involvement in disease

Charcot-Marie-Tooth disease 2P (CMT2P):
An axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal degeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.

PTM

Ubiquitination promoted by PHF23 leads to proteasomal degradation.

Lin, X., Tian, X., Jiang, H., Li, W., Wang, C., Wu, J., ... & Zwingenberger, S. (2023). Carpaine alleviates tendinopathy in mice by promoting the ubiquitin-proteasomal degradation of p65 via targeting the E3 ubiquitin ligase LRSAM1. Phytomedicine, 155323.

Li, Q., Wang, Y., Liu, F., Wang, H., & Fan, Y. (2022). LRSAM1 E3 Ubiquitin Ligase Promotes Choriocarcinoma Progression and Metastasis via p53/p21 Signaling Impediment. BioMed Research International, 2022.

Palaima, P., Berciano, J., Peeters, K., & Jordanova, A. (2021). LRSAM1 and the RING domain: Charcot–Marie–Tooth disease and beyond. Orphanet Journal of Rare Diseases, 16(1), 1-12.

Mishra, R., Joshi, V., Upadhyay, A., Amanullah, A., Dubey, A. R., Singh, S., ... & Mishra, A. (2021). LRSAM1 E3 ubiquitin ligase promotes proteasomal clearance of E6-AP protein. Cellular Signalling, 77, 109836.

Mishra, R., Amanullah, A., Upadhyay, A., Dhiman, R., Dubey, A. R., Singh, S., ... & Mishra, A. (2020). Ubiquitin ligase LRSAM1 suppresses neurodegenerative diseases linked aberrant proteins induced cell death. The International Journal of Biochemistry & Cell Biology, 120, 105697.

Yin, Z., Huang, G., Gu, C., Liu, Y., Yang, J., & Fei, J. (2020). Discovery of berberine that targetedly induces autophagic degradation of both BCR-ABL and BCR-ABL T315I through recruiting LRSAM1 for overcoming imatinib resistance. Clinical Cancer Research, 26(15), 4040-4053.

Minaidou, A., Nicolaou, P., & Christodoulou, K. (2019). Deregulation of LRSAM1 expression impairs the levels of TSG101, UBE2N, VPS28, MDM2 and EGFR. Plos one, 14(2), e0211814.

Mishra, R., Upadhyay, A., Prajapati, V. K., Dhiman, R., Poluri, K. M., Jana, N. R., & Mishra, A. (2019). LRSAM1 E3 ubiquitin ligase: molecular neurobiological perspectives linked with brain diseases. Cellular and Molecular Life Sciences, 76, 2093-2110.

Hu, B., Cao, L., Wang, X. Y., & Li, L. (2019). Downregulation of micro RNA‐431‐5p promotes enteric neural crest cell proliferation via targeting LRSAM 1 in Hirschsprung's disease. Development, growth & differentiation, 61(4), 294-302.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
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For research use only. Not intended for any clinical use.

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