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Mouse Anti-LYST Recombinant Antibody (CBYJL-2257) (CBMAB-L2517-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Lysosomal Trafficking Regulator (LYST). The antibody can be used for immunoassay techniques, such as ELISA.
See all LYST antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJL-2257
Antibody Isotype
IgG2b, κ
Application
ELISA

Basic Information

Immunogen
Partial recombinant corresponding to aa 1-100 from human LYST (NP_000072.2) with GST tag. MW of the GST tag alone is 26kD.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.2
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-100

Target

Full Name
lysosomal trafficking regulator
Introduction
LYST is a protein that regulates intracellular protein trafficking in endosomes, and may be involved in pigmentation. Mutations in this gene are involved in Chediak-Higashi syndrome, a lysosomal storage disorder. Alternative splicing results in multiple transcript variants, though the full-length nature of some of these variants has not been determined.
Entrez Gene ID
1130
UniProt ID
Q99698
Alternative Names
CHS; CHS1; lysosomal-trafficking regulator; Chediak-Higashi syndrome 1; beige homolog
Function
Adapter protein that regulates and/or fission of intracellular vesicles such as lysosomes (PubMed:11984006, PubMed:25216107).
Might regulate trafficking of effectors involved in exocytosis (PubMed:25425525).
In cytotoxic T-cells and natural killer (NK) cells, has role in the regulation of size, number and exocytosis of lytic granules (PubMed:26478006).
In macrophages and dendritic cells, regulates phagosome maturation by controlling the conversion of early phagosomal compartments into late phagosomes (By similarity).
In macrophages and dendritic cells, specifically involved in TLR3- and TLR4-induced production of pro-inflammatory cytokines by regulating the endosomal TLR3- TICAM1/TRIF and TLR4- TICAM1/TRIF signaling pathways (PubMed:27881733).
Biological Process
Defense response to bacteriumISS:UniProtKB
Defense response to protozoanISS:UniProtKB
Defense response to virusISS:UniProtKB
Endosome to lysosome transport via multivesicular body sorting pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Leukocyte chemotaxisISS:UniProtKB
Lysosome organizationIEA:InterPro
Mast cell secretory granule organizationISS:UniProtKB
Melanosome organizationISS:UniProtKB
Natural killer cell mediated cytotoxicityManual Assertion Based On ExperimentIMP:UniProtKB
PhagocytosisIEA:UniProtKB-KW
PigmentationManual Assertion Based On ExperimentIMP:UniProtKB
Protein localizationManual Assertion Based On ExperimentIBA:GO_Central
Protein transportIEA:UniProtKB-KW
Cellular Location
Cytoplasm
Involvement in disease
Chediak-Higashi syndrome (CHS):
A rare autosomal recessive disorder characterized by hypopigmentation, severe immunologic deficiency, a bleeding tendency, neurologic abnormalities, abnormal intracellular transport to and from the lysosome, and giant inclusion bodies in a variety of cell types. Most patients die at an early age unless they receive an allogeneic hematopoietic stem cell transplant (SCT).

Serra-Vinardell, J., Sandler, M. B., De Pace, R., Manzella-Lapeira, J., Cougnoux, A., Keyvanfar, K., ... & Malicdan, M. C. V. (2023). LYST deficiency impairs autophagic lysosome reformation in neurons and alters lysosome number and size. Cellular and Molecular Life Sciences, 80(2), 53.

Braulke, T., Carette, J. E., & Palm, W. (2023). Lysosomal enzyme trafficking: from molecular mechanisms to human diseases. Trends in Cell Biology.

Barker, J. C., Zbinden, J., Mirhaidari, G., Blum, K., & Breuer, C. (2021). QS10: The Lysosomal Trafficking Regulator (LYST) Protein is a Novel and Essential Mediator of Cutaneous Wound Healing. Plastic and Reconstructive Surgery–Global Open, 9(7S), 11-12.

Buckley, R. M., Grahn, R. A., Gandolfi, B., Herrick, J. R., Kittleson, M. D., Bateman, H. L., ... & Lyons, L. A. (2020). Assisted reproduction mediated resurrection of a feline model for Chediak-Higashi syndrome caused by a large duplication in LYST. Scientific reports, 10(1), 64.

Ullate-Agote, A., Burgelin, I., Debry, A., Langrez, C., Montange, F., Peraldi, R., ... & Tzika, A. C. (2020). Genome mapping of a LYST mutation in corn snakes indicates that vertebrate chromatophore vesicles are lysosome-related organelles. Proceedings of the National Academy of Sciences, 117(42), 26307-26317.

Lie, P. P., & Nixon, R. A. (2019). Lysosome trafficking and signaling in health and neurodegenerative diseases. Neurobiology of disease, 122, 94-105.

Ishii, S., Matsuura, A., & Itakura, E. (2019). Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe. Scientific Reports, 9(1), 11635.

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For research use only. Not intended for any clinical use.

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