Mouse Anti-LYST Recombinant Antibody (CBYJL-2257) (CBMAB-L2517-YJ)

Name: Mouse Anti-LYST Recombinant Antibody (CBYJL-2257)
SKU: CBMAB-L2517-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJL-2257

Application

ELISA

Immunogen

Partial recombinant corresponding to aa 1-100 from human LYST (NP_000072.2) with GST tag. MW of the GST tag alone is 26kD.

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.2

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 1-100

More Infomation

Target

Full Name

lysosomal trafficking regulator

Introduction

LYST is a protein that regulates intracellular protein trafficking in endosomes, and may be involved in pigmentation. Mutations in this gene are involved in Chediak-Higashi syndrome, a lysosomal storage disorder. Alternative splicing results in multiple transcript variants, though the full-length nature of some of these variants has not been determined.

Entrez Gene ID

1130

UniProt ID

Q99698

Alternative Names

CHS; CHS1; lysosomal-trafficking regulator; Chediak-Higashi syndrome 1; beige homolog

Function

Adapter protein that regulates and/or fission of intracellular vesicles such as lysosomes (PubMed:11984006, PubMed:25216107).
Might regulate trafficking of effectors involved in exocytosis (PubMed:25425525).
In cytotoxic T-cells and natural killer (NK) cells, has role in the regulation of size, number and exocytosis of lytic granules (PubMed:26478006).
In macrophages and dendritic cells, regulates phagosome maturation by controlling the conversion of early phagosomal compartments into late phagosomes (By similarity).
In macrophages and dendritic cells, specifically involved in TLR3- and TLR4-induced production of pro-inflammatory cytokines by regulating the endosomal TLR3- TICAM1/TRIF and TLR4- TICAM1/TRIF signaling pathways (PubMed:27881733).

Biological Process

Defense response to bacteriumISS:UniProtKB
Defense response to protozoanISS:UniProtKB
Defense response to virusISS:UniProtKB
Endosome to lysosome transport via multivesicular body sorting pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Leukocyte chemotaxisISS:UniProtKB
Lysosome organizationIEA:InterPro
Mast cell secretory granule organizationISS:UniProtKB
Melanosome organizationISS:UniProtKB
Natural killer cell mediated cytotoxicityManual Assertion Based On ExperimentIMP:UniProtKB
PhagocytosisIEA:UniProtKB-KW
PigmentationManual Assertion Based On ExperimentIMP:UniProtKB
Protein localizationManual Assertion Based On ExperimentIBA:GO_Central
Protein transportIEA:UniProtKB-KW

Cellular Location

Cytoplasm

Involvement in disease

Chediak-Higashi syndrome (CHS):
A rare autosomal recessive disorder characterized by hypopigmentation, severe immunologic deficiency, a bleeding tendency, neurologic abnormalities, abnormal intracellular transport to and from the lysosome, and giant inclusion bodies in a variety of cell types. Most patients die at an early age unless they receive an allogeneic hematopoietic stem cell transplant (SCT).

Serra-Vinardell, J., Sandler, M. B., De Pace, R., Manzella-Lapeira, J., Cougnoux, A., Keyvanfar, K., ... & Malicdan, M. C. V. (2023). LYST deficiency impairs autophagic lysosome reformation in neurons and alters lysosome number and size. Cellular and Molecular Life Sciences, 80(2), 53.

Braulke, T., Carette, J. E., & Palm, W. (2023). Lysosomal enzyme trafficking: from molecular mechanisms to human diseases. Trends in Cell Biology.

Barker, J. C., Zbinden, J., Mirhaidari, G., Blum, K., & Breuer, C. (2021). QS10: The Lysosomal Trafficking Regulator (LYST) Protein is a Novel and Essential Mediator of Cutaneous Wound Healing. Plastic and Reconstructive Surgery–Global Open, 9(7S), 11-12.

Buckley, R. M., Grahn, R. A., Gandolfi, B., Herrick, J. R., Kittleson, M. D., Bateman, H. L., ... & Lyons, L. A. (2020). Assisted reproduction mediated resurrection of a feline model for Chediak-Higashi syndrome caused by a large duplication in LYST. Scientific reports, 10(1), 64.

Ullate-Agote, A., Burgelin, I., Debry, A., Langrez, C., Montange, F., Peraldi, R., ... & Tzika, A. C. (2020). Genome mapping of a LYST mutation in corn snakes indicates that vertebrate chromatophore vesicles are lysosome-related organelles. Proceedings of the National Academy of Sciences, 117(42), 26307-26317.

Lie, P. P., & Nixon, R. A. (2019). Lysosome trafficking and signaling in health and neurodegenerative diseases. Neurobiology of disease, 122, 94-105.

Ishii, S., Matsuura, A., & Itakura, E. (2019). Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe. Scientific Reports, 9(1), 11635.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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