Mouse Anti-MAFK Recombinant Antibody (CBFYM-1288) (CBMAB-M1447-FY)

Name: Mouse Anti-MAFK Recombinant Antibody (CBFYM-1288)
SKU: CBMAB-M1447-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-1288

Application

Immunogen

KLH conjugated synthetic peptide between amino acids from human region of human MAFK

Specificity

Human

Antibody Isotype

IgG1, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

MAF BZIP TRANSCRIPTION FACTOR K

Introduction

The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions. LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2. NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase and ferrochelatase. NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein, or MafK) and p45. Both subunits are members of the activator protein-1 superfamily of basic leucine zipper proteins. Maf homodimers suppress transcription at NFE2 sites.

Entrez Gene ID

7975

UniProt ID

O60675

Alternative Names

MAF BZIP Transcription Factor K; Erythroid Transcription Factor NF-E2 P18 Subunit; V-Maf Avian Musculoaponeurotic Fibrosarcoma Oncogene Homolog K; Basic-Leucine Zipper Transcription Factor MafK; Nuclear Factor Erythroid-2, Ubiquitous (P18); V-Maf Avian Musculoaponeurotic Fibrosarcoma Oncogene Family, Protein K

Function

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:9150357).
However, they act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites (PubMed:9150357, PubMed:8932385).
Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor (PubMed:9150357).

Biological Process

Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:NTNU_SB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus

Patel, S. D., Anand, D., Motohashi, H., Katsuoka, F., Yamamoto, M., & Lachke, S. A. (2022). Deficiency of the bZIP transcription factors Mafg and Mafk causes misexpression of genes in distinct pathways and results in lens embryonic developmental defects. Frontiers in Cell and Developmental Biology, 10, 981893.

Si, N., Song, Z., Meng, X., Li, X., Xiao, W., & Zhang, X. (2019). A novel MAF missense mutation leads to congenital nuclear cataract by impacting the transactivation of crystallin and noncrystallin genes. Gene, 692, 113-118.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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