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Mouse Anti-MAGEA3 Recombinant Antibody (CBFYM-1310) (CBMAB-M1469-FY)

This product is mouse antibody that recognizes MAGEA3. The antibody CBFYM-1310 can be used for immunoassay techniques such as: ELISA, IHC, WB.
See all MAGEA3 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Pig, Rat
Clone
CBFYM-1310
Antibody Isotype
IgG1
Application
ELISA, IHC, WB

Basic Information

Specificity
Human, Mouse, Pig, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
MAGE Family Member A3
Introduction
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita.
Entrez Gene ID
Human4102
Mouse17139
UniProt ID
HumanP43357
MouseQ6T340
Alternative Names
MAGE Family Member A3; MAGE-3 Antigen; Antigen MZ2-D; Cancer/Testis Antigen Family 1, Member 3; Melanoma-Associated Antigen 3; Melanoma Antigen Family A3; Cancer/Testis Antigen 1.3
Function
Activator of ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases that acts as a as repressor of autophagy (PubMed:20864041, PubMed:31267705).
May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex (PubMed:17942928, PubMed:20864041).
May play a role in embryonal development and tumor transformation or aspects of tumor progression (PubMed:17942928, PubMed:20864041).
In vitro promotes cell viability in melanoma cell lines (PubMed:17942928).
Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes (PubMed:8113684).
Biological Process
Negative regulation of autophagyManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic processManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathwayManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Negative regulation of protein processingManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Endoplasmic reticulum
Nucleus
PTM
Ubiquitinated by the DCX(DCAF12) complex specifically recognizes the diglutamate (Glu-Glu) at the C-terminus, leading to its degradation.

Eakins, R. A., Chobrutskiy, A., Teer, J. K., Patel, D. N., Hsiang, M., Huda, T. I., ... & Chobrutskiy, B. I. (2022). Chemical complementarity between tumor resident, T-cell receptor CDR3s and MAGEA3/6 correlates with increased melanoma survival: Potential relevance to MAGE vaccine auto-reactivity. Molecular immunology, 150, 58-66.

Craig, A. J., Garcia-Lezana, T., Ruiz de Galarreta, M., Villacorta-Martin, C., Kozlova, E. G., Martins-Filho, S. N., ... & Villanueva, A. (2021). Transcriptomic characterization of cancer-testis antigens identifies MAGEA3 as a driver of tumor progression in hepatocellular carcinoma. PLoS genetics, 17(6), e1009589.

Martin, A. D., Wang, X., Sandberg, M. L., Negri, K. R., Wu, M. L., Warshaviak, D. T., ... & Kamb, A. (2021). Re-examination of MAGE-A3 as a T-cell therapeutic target. Journal of Immunotherapy (Hagerstown, Md.: 1997), 44(3), 95.

Das, B., & Senapati, S. (2021). Immunological and functional aspects of MAGEA3 cancer/testis antigen. Advances in Protein Chemistry and Structural Biology, 125, 121-147.

Chen, Y., Zhao, H., Li, H., Feng, X., Tang, H., Zhang, J., ... & Qiu, C. (2020). LINC01234/MicroRNA-31-5p/MAGEA3 axis mediates the proliferation and chemoresistance of hepatocellular carcinoma cells. Molecular therapy. Nucleic acids, 19, 168.

Gao, X., Li, Q., Chen, G., He, H., & Ma, Y. (2020). MAGEA3 promotes proliferation and suppresses apoptosis in cervical cancer cells by inhibiting the KAP1/p53 signaling pathway. American Journal of Translational Research, 12(7), 3596.

Das, B., & Senapati, S. (2019). Functional and mechanistic studies reveal MAGEA3 as a pro-survival factor in pancreatic cancer cells. Journal of Experimental & Clinical Cancer Research, 38(1), 1-18.

Pol, J. G., Acuna, S. A., Yadollahi, B., Tang, N., Stephenson, K. B., Atherton, M. J., ... & McCart, J. A. (2019). Preclinical evaluation of a MAGE-A3 vaccination utilizing the oncolytic Maraba virus currently in first-in-human trials. Oncoimmunology, 8(1), e1512329.

Conley, A. P., Wang, W. L., Livingston, J. A., Ravi, V., Tsai, J. W., Ali, A., ... & Roszik, J. (2019). MAGE-A3 is a clinically relevant target in undifferentiated pleomorphic sarcoma/myxofibrosarcoma. Cancers, 11(5), 677.

Ravichandran, R., Kodali, K., Peng, J., & Potts, P. R. (2019). Regulation of MAGE‐A3/6 by the CRL 4‐DCAF 12 ubiquitin ligase and nutrient availability. EMBO reports, 20(7), e47352.

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For research use only. Not intended for any clinical use.

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