Mouse Anti-MAGEB4 (AA 1-193 ) Recombinant Antibody (CBFYM-1324) (CBMAB-M1483-FY)

This product is mouse antibody that recognizes MAGEB4. The antibody CBFYM-1324 can be used for immunoassay techniques such as: WB, IHC.
See all MAGEB4 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYM-1324

Antibody Isotype

IgG1

Application

WB, IHC

Basic Information

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-193 of human MAGEB4(NP_002358) produced in E.coli

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA

Preservative

0.02% Sodium azide

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-193

Target

Full Name

MAGE FAMILY MEMBER B4

Introduction

This gene is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEB genes are clustered on chromosome Xp22-p21. This gene sequence ends in the first intron of MAGEB1, another family member. This gene is expressed in testis.

Entrez Gene ID

4115

UniProt ID

O15481

Alternative Names

MAGE Family Member B4; Cancer/Testis Antigen Family 3, Member 6; Melanoma-Associated Antigen B4; Melanoma Antigen Family B4; MAGE-B4 Antigen; Melanoma Antigen Family B, 4; CT3.6

Biological Process

Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm

References

Li, N., Yu, J., Zhou, M., Qiu, F., Wang, X., & Wang, Z. (2023). MAGE-B4, a binding partner of PRAMEF12, is dispensable for spermatogenesis and male fertility in mice. Biochemical and Biophysical Research Communications, 675, 46-53.

Gao, S., Yun, D. M., Zhou, L. W., Wu, Y. H., Lin, D. F., Wu, X. L., & Sun, F. (2020). Mageb4, a testis-specific gene, is dispensable for mouse spermatogenesis. Reproductive and Developmental Medicine, 4(03), 129-136.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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