Sign in or Register   Sign in or Register
  |  

Mouse Anti-MAP1LC3A Recombinant Antibody (A870) (CBMAB-AP11378LY)

The product is antibody recognizes LC3A. The antibody A870 immunoassay techniques such as: IF, IHC, WB.
See all MAP1LC3A antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
A870
Antibody Isotype
IgG
Application
IF, IHC, WB

Basic Information

Immunogen
Synthetic Peptide of LC3A
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
Affinity purity
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Microtubule Associated Protein 1 Light Chain 3 Alpha
Entrez Gene ID
Human84557
Mouse66734
Rat362245
UniProt ID
HumanQ9H492
MouseQ91VR7
RatQ6XVN8
Alternative Names
Microtubule-associated proteins 1A/1B light chain 3A (Autophagy-related protein LC3 A) (Autophagy-related ubiquitin-like modifier LC3 A) (MAP1 light chain 3-like protein 1) (MAP1A/MAP1B light chain 3 A) (MAP1A/MAP1B LC3 A) (Microtubule-associated protein 1 light chain 3 alpha)
Function
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141).
While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600).
Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538, PubMed:31006537).
Biological Process
Autophagosome assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Autophagosome maturationManual Assertion Based On ExperimentIDA:UniProtKB
Autophagy of mitochondrionManual Assertion Based On ExperimentIGI:MGI
Cellular response to amino acid starvationIEA:Ensembl
Cellular response to copper ionIEA:Ensembl
Cellular response to hydrogen peroxideIEA:Ensembl
Cellular response to nitrogen starvationManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to starvationManual Assertion Based On ExperimentIDA:UniProtKB
MacroautophagyManual Assertion Based On ExperimentIDA:UniProtKB
Response to iron(II) ionIEA:Ensembl
Response to lead ionIEA:Ensembl
Response to morphineIEA:Ensembl
Cellular Location
Cytoplasmic vesicle, autophagosome membrane
Endomembrane system
Cytoplasm, cytoskeleton
LC3-II binds to the autophagic membranes.
PTM
The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, LC3-I (PubMed:15187094, PubMed:30661429).
The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094).
During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid (PubMed:33909989).
ATG4 proteins also mediate the delipidation of PE-conjugated forms (PubMed:32686895, PubMed:33909989).
In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy (PubMed:33909989).
ATG4B constitutes the major protein for proteolytic activation (PubMed:30661429, PubMed:33909989).
ATG4D is the main enzyme for delipidation activity (By similarity).
(Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine (PubMed:23112293, PubMed:32686895, PubMed:31722778).
RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS) (PubMed:33909989).
Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE).
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-MAP1LC3A Recombinant Antibody (A870)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare