Mouse Anti-MAP1LC3B2 Recombinant Antibody (CBFYM-1392) (CBMAB-M1552-FY)

Name: Mouse Anti-MAP1LC3B2 Recombinant Antibody (CBFYM-1392)
SKU: CBMAB-M1552-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBFYM-1392

Application

WB, IP, IF, ELISA

Specificity

Mouse, Rat, Human

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

MICROTUBULE ASSOCIATED PROTEIN 1 LIGHT CHAIN 3 BETA 2

Introduction

MAP1LC3B2 is a Protein Coding gene. Gene Ontology annotations related to this gene include microtubule binding. An important paralog of this gene is MAP1LC3B.

Entrez Gene ID

Human	643246
Rat	100359928

UniProt ID

Human	A6NCE7
Rat	Q7TQ75

Alternative Names

Microtubule Associated Protein 1 Light Chain 3 Beta 2; Microtubule-Associated Proteins 1A/1B Light Chain 3B-Like Protein; Microtubule-Associated Proteins 1A/1B Light Chain 3 Beta 2; Microtubule-Associated Proteins 1A/1B Light Chain 3B-Like; ATG8G

Function

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria. While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

Biological Process

Autophagosome assemblyManual Assertion Based On ExperimentIBA:GO_Central
Autophagosome maturationManual Assertion Based On ExperimentIBA:GO_Central
Autophagy of mitochondrionManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to nitrogen starvationManual Assertion Based On ExperimentIBA:GO_Central
MacroautophagyManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasmic vesicle, autophagosome membrane
Endomembrane system
Cytoplasm, cytoskeleton

PTM

The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, LC3-I (PubMed:30661429).
The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, LC3-II (By similarity).
During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid (By similarity).
ATG4 proteins also mediate the delipidation of PE-conjugated forms (By similarity).
In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy (By similarity).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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