Mouse Anti-MAP2K3 Recombinant Antibody (CBFYM-1470) (CBMAB-M1630-FY)

Name: Mouse Anti-MAP2K3 Recombinant Antibody (CBFYM-1470)
SKU: CBMAB-M1630-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-1470

Application

ELISA, WB, IHC

Immunogen

Recombinant protein

Specificity

Human

Antibody Isotype

IgG1, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Mitogen-Activated Protein Kinase Kinase 3

Introduction

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.

Entrez Gene ID

5606

UniProt ID

P46734

Alternative Names

Mitogen-Activated Protein Kinase Kinase 3; MAP Kinase Kinase 3; MAPK/ERK Kinase 3; Stress-Activated Protein Kinase Kinase 2; SAPK Kinase 2; EC 2.7.12.2; MAPKK 3; SAPKK-2; PRKMK3

Function

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.

Biological Process

Cardiac muscle contractionIEA:Ensembl
Cellular response to lipopolysaccharideIEA:Ensembl
Cellular response to sorbitolIEA:Ensembl
Cellular response to vascular endothelial growth factor stimulusManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular senescenceTAS:Reactome
Heart developmentIEA:Ensembl
Inflammatory responseIEA:Ensembl
MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of hippo signalingManual Assertion Based On ExperimentIMP:FlyBase
p38MAPK cascadeManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of blood vessel endothelial cell migrationManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of MAPK cascadeIEA:Ensembl
Positive regulation of nitric-oxide synthase biosynthetic processIEA:Ensembl
Positive regulation of protein kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cytokine productionIEA:Ensembl
Response to ischemiaIEA:Ensembl
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Cytosol
Membrane
Nucleoplasm

Involvement in disease

Defects in MAP2K3 may be involved in colon cancer.

PTM

Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity (PubMed:8622669).
Phosphorylated by TAOK2 (PubMed:11279118).
(Microbial infection) Yersinia YopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.

Gu, K., Wang, T., Peng, L., & Zhao, Y. (2022). FIP-fve stimulates cell proliferation and enhances IL-2 release by activating MAP2K3/p38α (MAPK14) signaling pathway in Jurkat E6-1 cells. Frontiers in Nutrition, 9, 881924.

Bhargavi, M., Vhora, N., Lanka, G., Somadi, G., Kanth, S. S., Jain, A., & Potlapally, S. R. (2021). Homology modelling and virtual screening to explore potent inhibitors for MAP2K3 protein. Structural Chemistry, 32, 1039-1051.

Zhang, Y., Lu, W., Chen, Y., Lin, Y., Yang, X., Wang, H., & Liu, Z. (2021). The miR‐19b‐3p‐MAP2K3‐STAT3 feedback loop regulates cell proliferation and invasion in esophageal squamous cell carcinoma. Molecular Oncology, 15(5), 1566-1583.

Sawada, Y., Nakatsuji, T., Dokoshi, T., Kulkarni, N. N., Liggins, M. C., Sen, G., & Gallo, R. L. (2021). Cutaneous innate immune tolerance is mediated by epigenetic control of MAP2K3 by HDAC8/9. Science immunology, 6(59), eabe1935.

Qi, A., Wang, T., Li, W., Wang, Y., & Chai, Y. (2021). The effect of miR-21-5p on the MAP2K3 expressions and cellular apoptosis in the lung tissues of neonatal rats with hyperoxia-induced lung injuries. American Journal of Translational Research, 13(4), 2784.

Kim, Y. J., Jung, E., Shin, E., Hong, S. H., Jeong, H. S., Hur, G., ... & Kim, J. (2020). Genome-wide RNA interference screening reveals a COPI-MAP2K3 pathway required for YAP regulation. Proceedings of the National Academy of Sciences, 117(33), 19994-20003.

Gholipoorfeshkecheh, R., Agarwala, S., Krishnappa, S., Savitha, M. R., & Ramachandra, N. B. (2020). Whole-exome sequencing and homozygosity mapping identify variants in NCOR1 and MAP2K3 associated with non-syndromic congenital heart defects. Egyptian Journal of Medical Human Genetics, 21(1), 1-10.

Han, S., Wang, Y., Ma, J., Wang, Z., Wang, H. M. D., & Yuan, Q. (2020). Sulforaphene inhibits esophageal cancer progression via suppressing SCD and CDH3 expression, and activating the GADD45B-MAP2K3-p38-p53 feedback loop. Cell death & disease, 11(8), 713.

He, F., Xiao, Z., Yao, H., Li, S., Feng, M., Wang, W., ... & Wu, J. (2019). The protective role of microRNA-21 against coxsackievirus B3 infection through targeting the MAP2K3/P38 MAPK signaling pathway. Journal of translational medicine, 17(1), 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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