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Mouse Anti-MAP3K4 Recombinant Antibody (6C6) (CBMAB-A5213-LY)

The product is antibody recognizes MAP3K4. The antibody 6C6 immunoassay techniques such as: WB, ELISA.
See all MAP3K4 antibodies
Published Data
Fig.1 Phosphorylation and activation of MKK6 and SEK1 by MTK1 in vitro.
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Fig.2 MTK1 functionally complements ssk2 ssk22 sho1 deficiency and specifically activates the HOG pathway in S.cerevisiae.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
6C6
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
MAP3K4 (NP_005913, 1201 a.a. ~ 1300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
mitogen-activated protein kinase kinase kinase 4
Introduction
The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq]
Entrez Gene ID
4216
UniProt ID
Q9Y6R4
Alternative Names
FLJ42439; KIAA0213; MAPKKK4; MEKK4; MTK1; PRO0412
Function
Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.
Biological Process
Chorionic trophoblast cell differentiationIEA:Ensembl
Intracellular signal transductionISS:UniProtKB
Male germ-line sex determinationIEA:Ensembl
MAPK cascadeManual Assertion Based On ExperimentIDA:UniProtKB
Placenta developmentIEA:Ensembl
Positive regulation of JUN kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of p38MAPK cascadeManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of telomerase activityManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of telomere cappingManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of telomere maintenance via telomeraseManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of gene expressionIEA:Ensembl
Response to UV-CManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Cytoplasm, perinuclear region
Localized in perinuclear vesicular-like structures, probably Golgi-associated vesicles.

Qu, F., Li, J., Zeng, X., She, Q., Li, Y., Xie, W., ... & Liu, Z. (2022). Grass carp Map3k4 participates in the intestinal immune response to bacterial challenge. Fish & Shellfish Immunology, 120, 82-91.

Sun, Y., Lv, Y., Ren, H. W., Wang, G. Y., Xuan, L. N., Luo, Y. Y., & Luan, Z. L. (2022). Association between MAP3K4 gene polymorphisms and the risk of schizophrenia susceptibility in a Northeast Chinese Han population. Metabolic Brain Disease, 37(5), 1365-1371.

Perry, C. H., Mullins, N. A., Sweileh, R. B., Shendy, N. A., Roberto, P. A., Broadhurst, A. L., ... & Abell, A. N. (2022). MAP3K4 promotes fetal and placental growth by controlling the receptor tyrosine kinases IGF1R/IR and Akt signaling pathway. Journal of Biological Chemistry, 298(9).

He, Z., Bin, Y., Chen, G., Li, Q., Fan, W., Ma, Y., ... & Li, J. (2022). Identification of MAP3K4 as a novel regulation factor of hepatic lipid metabolism in non-alcoholic fatty liver disease. Journal of Translational Medicine, 20(1), 1-13.

Zhou, H., Simion, V., Pierce, J. B., Haemmig, S., Chen, A. F., & Feinberg, M. W. (2021). LncRNA‐MAP3K4 regulates vascular inflammation through the p38 MAPK signaling pathway and cis‐modulation of MAP3K4. The FASEB Journal, 35(1), e21133.

Shendy, N. A., Broadhurst, A. L., Shoemaker, K., Read, R., & Abell, A. N. (2021). MAP3K4 kinase activity dependent control of mouse gonadal sex determination. Biology of Reproduction, 105(2), 491-502.

Paranjpe, M., Yu, H., Frankenberg, S., Pask, A. J., Shaw, G., & Renfree, M. B. (2020). Transcriptomic analysis of MAP3K1 and MAP3K4 in the developing marsupial gonad. Sexual Development, 13(4), 195-204.

Huang, L., & Wang, L. (2020). Upregulation of miR‐183 represses neuropathic pain through inhibiton of MAP3K4 in CCI rat models. Journal of Cellular Physiology, 235(4), 3815-3822.

Zhao, L. L., Liu, H. L., Luo, S., Walsh, K. M., Li, W., & Wei, Q. (2020). Associations of novel variants in PIK3C3, INSR and MAP3K4 of the ATM pathway genes with pancreatic cancer risk. American journal of cancer research, 10(7), 2128.

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For research use only. Not intended for any clinical use.

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